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Lead and cadmium tolerant bacteria and method for plants renovation of soil pollution by heavy metal

A heavy metal and cadmium resistance technology, applied in the fields of agriculture and environmental pollution control, can solve the problems of low biomass, complex equipment, inability to absorb and enrich, etc., to achieve the goal of promoting plant growth, improving repair efficiency, and improving plant stress resistance Effect

Inactive Publication Date: 2008-04-02
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the commonly used physical and physicochemical remediation methods are generally expensive in investment, complex in equipment, and easily lead to soil structure damage; and phytoextraction remediation technology is usually short, slow in growth, and low in biomass due to hyperaccumulative plants, which cannot be used for lead and cadmium in the stationary phase. The disadvantages such as absorption and enrichment limit the application in actual production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Insoluble heavy metal lead and cadmium in the activation solution of embodiment 1 bacterial strain J62

[0019] Fill 100ml of PbCO into a 250ml Erlenmeyer flask 3 0.064g and CdCO 3 0.077g nitrogen medium (sucrose 10g, (NH 4 ) 2 SO 4 1g, KH 2 PO 4 2g, MgSO 4 0.5g, NaCl 0.1g, CaCO 3 0.5g, yeast extract 0.5g), sterilized at 121°C for 30min. The strain J62 was inoculated, cultured on a shaker at 30°C and 150r / min, and samples were taken and analyzed at different time points. Measure the number of bacterial strains by dilution coating method; measure the pH value in the culture solution with a pH meter; centrifuge the culture solution, take the supernatant and use the atomic absorption method to determine Pb 2+ and Cd 2+ concentration. The results are shown in Table 1. Effect of strain J62 on PbCO in culture medium 3 and CdCO 3 The activation efficiency can reach 1.5-6 times and 2.7-17.2 times.

Embodiment 2

[0020] Example 2 Bacterial strain J62 activates soil heavy metal lead and cadmium

[0021] Accurately weigh 10.00 g of a soil sample containing lead carbonate (Pb 500 mg / kg) and cadmium carbonate (Cd 500 mg. / kg), add it to a 50 ml centrifuge tube, wrap the mouth with gauze, and sterilize under high pressure. Bacteria strain J62 was cultured on a shaker at 28°C for 20 hours, the bacteria were collected by centrifugation, and the bacteria suspension was made with deionized water. Inoculate 3ml of bacterial suspension into the soil sample, take the same amount of deionized water and inactivated bacteria as the control, set 3 repetitions for each treatment, and replenish water regularly. The centrifuge tubes were placed in an incubator at 28°C for 3d, 7d, 13d and 21d and then taken out. Weigh 2g of soil into a 7ml centrifuge tube, add 4ml of water, shake and mix for 2h, centrifuge to take the supernatant to measure the concentration of water-soluble heavy metals Pb and Cd. Weigh...

Embodiment 3

[0022] Embodiment 3 liquid preparation

[0023] The slant bacteria were inoculated in the nitrogen medium, cultured for 18 hours and then transferred to the seed tank. The seed tank is 0.5 tons, the feeding amount is 0.4 tons, and the medium composition is 2.0kg of sucrose, (NH 4 ) 2 SO 4 0.25kg, KH 2 PO 4 0.7kg, MgSO 4 0.17kg, NaCl 0.07kg, CaCO 3 0.4kg, yeast paste 0.2kg. The seed tank must be sterilized by steam and cooled to 28-30°C, the volume ratio of the inoculation volume is 5%, the cultivation temperature of the seed tank is controlled at 28-32°C, the stirring speed is 220 rpm, and the amount of sterile air is 1 : 0.8, after 20 hours of cultivation, the seed liquid was connected to the production tank. The production tank is 7 tons, the feeding amount is 4.5 tons, and the medium composition is 8kg of sucrose, 18kg of starch, (NH 4 ) 2 SO 4 4.5kg, KH 2 PO 4 9.0kg, MgSO 4 2.3kg, NaCl 0.9kg, CaCO 3 4.5kg, yeast paste 0.9kg. The production t...

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Abstract

The invention relates to a heavy metal lead and cadmium antibiotic-resistant bacterium and a method for strengthening lead and cadmium heavy metals in the vegetable extraction soil, and belongs to the agricultural and environmental pollution treatment technical field. The J62 culture stain is appraised as the Burkholderia sp, has antibiotic resistance to a plurality of heavy metals, in particular to lead and cadmium, respectively reaching Pb<2+>1000mg / L and Cd<2+>2000mg / L. The invention has the characteristics of nitrogen fixation and phosphate solubilizing, and can promote the growth of the plant and improve the stress resistance of the plant. The effective living microbial numbers of the liquid preparation are above a billion per millimeter, and the effective living microbial numbers of the solid preparation are 200 millions per gram. When the seeds of the plant are seminated in humid soil containing heavy metals, 10 to 20mL of the bacteria liquid of 10<8> bacteria J62 / mLs is inoculated in each grams of soil once or in two times. The invention promotes the growth of the plant, strengthens the enrichment of heavy metal lead and cadmium of the plant, and improves the extraction and recovery efficiency of the plant.

Description

1. Technical field [0001] The invention relates to a lead-cadmium-resistant bacterium and a phytoremediation method for soil heavy metal pollution, belonging to the technical field of agriculture and environmental pollution control, so as to realize phytoremediation of soil heavy metal pollution. 2. Technical background [0002] Because heavy metals are difficult to degrade, migrate and have a long period of harm in soil, soil heavy metal pollution has become one of the environmental problems that are widely concerned at home and abroad. Countries all over the world have attached great importance to heavy metal pollution and its treatment. However, the commonly used physical and physicochemical remediation methods are generally expensive in investment, complex in equipment, and easily lead to soil structure damage; and phytoextraction remediation technology is usually short, slow in growth, and low in biomass due to hyperaccumulative plants, which cannot be used for lead and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10
Inventor 盛下放何琳燕江春玉
Owner NANJING AGRICULTURAL UNIVERSITY
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