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Caesium-enriched microorganism and application thereof

A microbial enrichment technology, applied in the field of biological treatment of environmental pollution, can solve the problems of secondary pollution, difficult to control large-scale environmental pollution, and high cost

Inactive Publication Date: 2011-08-17
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Conventional methods used to control nuclide pollution include physical methods and chemical methods, such as zeolite adsorption, ion exchange, solvent extraction, etc., but these methods are costly, difficult to operate, and easy to cause secondary pollution, so it is difficult to Controlling large-area pollution in the environment

Method used

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  • Caesium-enriched microorganism and application thereof
  • Caesium-enriched microorganism and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0011] Example 1: Resistance of strain Burkholderia sp.D54 to cesium

[0012] The resistance of strain Burkholderia sp.D54 to cesium was determined by gradient plate method. Prepare 300 milliliters of solid improved SMN medium (prescription is: mannitol 1%; (NH 4 ) 2 SO 4 , 0.2%; peptone, 0.2%; K 2 HPO 4 , 0.05%; MgSO 4 , 0.05%; FeSO 4 , 0.005%; MnSO 4 , 0.005%; yeast extract, 0.05%; pH 7.0), transferred to a 500ml Erlenmeyer flask, 121°C, sterilized for 30 minutes, then accurately added cesium chloride solutions of a series of known concentrations that were sterilized separately according to the corresponding concentration, and fully oscillated Mix well so that the final cesium content of the medium is 0.2, 0.5, 1.0, 2.0, 4.0, 10.0, 20.0, 50.0 mM respectively. Invert the plate when the culture medium is cooled to 50°C. After the plate is cooled to room temperature, add 1% (v / v) logarithmic phase bacterial solution (the number of viable bacteria is about 1×10 8 ml -...

Embodiment 2

[0016] Example 2: Enrichment of Cesium by Strain Burkholderia sp.D54

[0017] The cesium-containing liquid medium was used to culture the strain to determine the cesium enrichment ability of the strain. In a 200mL Erlenmeyer flask, configure 95mL of liquid modified SMN medium, sterilize at 121°C for 30min, then add 5mL of cesium chloride solution with a cesium concentration of 20mM that is sterilized separately, shake and mix well, the concentration of cesium in the medium 1mM, after cooling the culture medium to room temperature, add 1% (v / v) logarithmic phase bacteria solution (the number of viable bacteria is about 1×10 8 ml -1 ), 30°C, 200 rpm, and cultivated for 3 days. Then the bacterial solution was centrifuged at 10,000 rpm at 10°C for 10 minutes, suspended in a sterile 0.85% sodium chloride solution and then centrifuged, washed twice, and dried at 80°C to a constant weight, and weighed . The weighed bacteria were digested by microwave (CEM, Mars: 240 / 50, America, ...

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Abstract

The invention belongs to the technical field of environment pollution biological treatment, relates to a microorganism repair technology and in particular relates to a method for enrichment and removal of caesium polluted environmental microorganisms. In the method, heavy metal resisting strain Burkholderia sp.D54 selected and separated from soil polluted by heavy metal is applied to repair of microorganisms polluted by radionuclide caesium. The strain Burkholderia sp.D54 can be grown in a culture medium containing 50mM of caesium; the strain Burkholderia sp.D54 is cultured in a culture medium containing 1mM of caesium for 3 days so that 0.1737+ / -0.0017g of thallus by dry weight; and the enrichment amount of the caesium reaches 45+ / -3.85mg g<-1> (dry weight thallus), and the removal rate of the caesium in the culture medium is 58.77%. The strain Burkholderia sp.D54 has strong appetency on radionuclide and can be applied to the repair of the environmental microorganisms polluted by the radionuclide caesium, thus the prospect of the strain is wide.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollution, and relates to microbial remediation technology, in particular to a method for enriching and removing microorganisms in a cesium-contaminated environment. Background technique [0002] Cesium (Cs + ) is the first group of alkali metal elements, chemical properties and potassium (K + )resemblance. Cesium exists in nature 133 Stable isotopes of Cs and radioactive artificial nuclides of cesium 134 Cs, 137 The half-lives of Cs are 2 and 30 years respectively, so radioactive 137 After Cs enters the environment, it can remain in the soil for a long time and cause pollution to the environment. In the 1950s and 1960s, due to nuclear weapons tests, especially after the Chernobyl nuclear leak in the former Soviet Union in 1986, a large amount of radioactivity 137 Cs is released into the soil and enters the Earth's cycle with water and air, polluting large area...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10
Inventor 唐世荣廖上强郭军康丁永祯居学海宋宁宁
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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