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A composite bacterium agent for reducing a doxycycline concentration and a drug-resistant gene in a composting process and applications thereof

A technology of compound bacterial agents and drug-resistant genes, applied in the direction of microorganism-based methods, bacteria, fungi, etc., to achieve high degradation effect, low content of drug-resistant genes, and improve safety

Inactive Publication Date: 2018-04-17
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings and deficiencies of antibiotics and drug-resistant gene residues in the existing feces, the primary purpose of the present invention is to provide a compound bacterial agent that reduces the concentration of doxycycline and drug-resistant genes in the composting process

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] (1) Preparation of compound bacterial agent: In this method, three strains of doxycycline-degrading bacteria, Ochrobactrum sp. DDM1, Burkholderia sp. DDM2 and Candida sp. DDM10, respectively placed in nutrient medium, cultured at 37°C (cultivation time: 24 hours for Pallidum pallidum, 96 hours for Burkholderia, and 24 hours for Candida), centrifuged a certain volume of culture medium at 6000 rpm for 10 minutes, and discarded the supernatant to obtain Thalline, after adding 1 / 5 times the volume of culture medium, skimmed milk powder was mixed, placed in a freeze dryer to freeze-dry, and the number of live bacteria in the freeze-dried powder of Paleobacterium pallidus, Burkholderia and Candida were 8.7×10 9 , 6.0×10 9 and 7.9×10 9 The freeze-dried powder of CFU / g is made into a composite bacterial agent according to the freeze-dried powder ratio of 40% of Pallidal bacillus, 40% of Burkholderia and 20% of Candida.

[0027] (2) Application of the compound bacterial agent:...

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Abstract

A composite bacterium agent for reducing a doxycycline concentration and a drug-resistant gene in a composting process and applications thereof are disclosed and belong to the technical fields of antibiotics and drug-resistant gene removing. The composite bacterium agent includes Ochrobactrum sp., Burkholderia sp., and Candida sp.. The mass ratio of freeze-dried powder of the Ochrobactrum sp., theBurkholderia sp., and the Candida sp. is 1-2:1-2:1-2. Through adding the composite bacterium agent into compost, residual doxycycline in livestock excrement can be efficiently degraded, the drug-resistant gene residual in the compost is effectively degraded, and safety of a compost product is improved. Through inoculation of the composite bacterium agent, the doxycycline degradation rate is 46.40%, and the tetracycline drug-resistant gene removal rate is 38.71%. Compost inoculated with the composite bacterium agent has higher doxycycline degradation effects and a lower drug-resistant gene content than compost not inoculated with the composite bacterium agent so that the composite bacterium agent has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of removing antibiotics and drug-resistant genes, and in particular relates to a compound microbial agent for reducing doxycycline concentration and drug-resistant genes in a composting process and an application thereof. Background technique [0002] With the large-scale breeding of livestock and poultry, the discharge of livestock and poultry manure is increasing day by day. Feces not only contain a large amount of organic matter and rich nutrients, but also contain harmful components such as antibiotics, heavy metals and various pathogenic bacteria. If they are not properly disposed of and used directly, it will cause secondary pollution to the environment. Composting can kill pathogenic bacteria, decompose organic matter and other advantages are widely used. However, the antibiotics and their resistance genes in the compost will not only affect the composting process, but also affect the quality and saf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C05F11/08C12R1/01C12R1/72
CPCC05F11/08C12N1/16C12N1/20
Inventor 吴银宝金益曼王燕廖新俤米见对曹俊超
Owner SOUTH CHINA AGRI UNIV
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