A composite bacterium agent for reducing a doxycycline concentration and a drug-resistant gene in a composting process and applications thereof
A technology of compound bacterial agents and drug-resistant genes, applied in the direction of microorganism-based methods, bacteria, fungi, etc., to achieve high degradation effect, low content of drug-resistant genes, and improve safety
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[0026] (1) Preparation of compound bacterial agent: In this method, three strains of doxycycline-degrading bacteria, Ochrobactrum sp. DDM1, Burkholderia sp. DDM2 and Candida sp. DDM10, respectively placed in nutrient medium, cultured at 37°C (cultivation time: 24 hours for Pallidum pallidum, 96 hours for Burkholderia, and 24 hours for Candida), centrifuged a certain volume of culture medium at 6000 rpm for 10 minutes, and discarded the supernatant to obtain Thalline, after adding 1 / 5 times the volume of culture medium, skimmed milk powder was mixed, placed in a freeze dryer to freeze-dry, and the number of live bacteria in the freeze-dried powder of Paleobacterium pallidus, Burkholderia and Candida were 8.7×10 9 , 6.0×10 9 and 7.9×10 9 The freeze-dried powder of CFU / g is made into a composite bacterial agent according to the freeze-dried powder ratio of 40% of Pallidal bacillus, 40% of Burkholderia and 20% of Candida.
[0027] (2) Application of the compound bacterial agent:...
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