Burkholderia LH-ZY01 and application thereof in suppressing rhizoctonia solani

A technology of Burkholderia and strains, applied in the field of microorganisms, can solve the problems of short survival period, limited application, poor stability, etc., and achieve the effect of long shelf life, promotion of crop growth, and good stability

Active Publication Date: 2013-11-27
山东鲁虹智慧农业研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since this type of strain is not Bacillus, it has the limitations of poor stability and short survival period when directly made into wettable powder, which greatly limits the application of this strain in agriculture.
In addition, another bottleneck in the application of microbial fertilizers is the colonization of beneficial microorganisms in the soil after fertilization

Method used

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  • Burkholderia LH-ZY01 and application thereof in suppressing rhizoctonia solani
  • Burkholderia LH-ZY01 and application thereof in suppressing rhizoctonia solani
  • Burkholderia LH-ZY01 and application thereof in suppressing rhizoctonia solani

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Burkholderia LH-ZY01 ( burkholderia sp. ) inhibitory effect on Rhizoctonia solani

[0033] 1. The strains of Rhizoctonia solani for testing

[0034] Rhizoctonia solani used for testing ( Rhizoctonia ), which was purchased from the Microbiology Collection Center of Beijing Institute of Microbiology.

[0035] 2. Burkholderia LH-ZY01 ( burkholderia sp. ) Plate confrontation experiment against Rhizoctonia solani

[0036]Pick the first-class seeds containing Burkholderia cultured on an inclined plane and transfer them to a shaking tube containing complete liquid medium, and place them on a shaker for cultivation. The specific cultivation conditions are: rotation speed 150 r / min, 30°C, 1d; at the same time, culture Rhizoctonia solani with PDA medium, specific conditions: rotation speed 150 r / min, 25°C, culture 2d.

[0037] Pipette 100 ul of the cultivated Rhizoctonia solani culture solution and add it to the prepared PDA plate to spread evenly, then use sterilized...

Embodiment 2

[0056] Pot experiments on the effect of fertilizer application using rapeseed.

[0057] According to the method of embodiment 1, the natural soil containing a large amount of Rhizoctonia solani was used to carry out the pot test. The experimental group was added with the prepared organic bacterial manure according to the addition amount of 90kg / mu; the control group was added with the same amount of decomposed manure without the target bacteria. The experimental group and the control group were sown with the same amount of rape seeds, watered normally, and the actual growth conditions were observed.

[0058] Experimental results

[0059] (1) Seven days after the seeds germinated, samples were taken from the roots of the plants to detect the added strain LH-ZY01 ( burkholderia sp. ) in the fixed value of crop roots and the existence of Rhizoctonia solani, the results are basically the same as in Example 1: LH-ZY01 ( burkholderia sp. ) strains existed in large numbers around ...

Embodiment 3

[0063] Pot experiment on fertilizer application effect using tomato

[0064] According to the method of embodiment 1, 2, the natural soil containing a large amount of Rhizoctonia solani was used to carry out the pot test. The experimental group added the prepared organic bacterial manure at an addition rate of 120kg / mu; the control group added the same amount of decomposed manure without the target bacteria. The experimental group and the control group were sown with the same amount of tomato seeds, watered normally, and the actual growth conditions were observed.

[0065] Experimental results

[0066] (1) Seven days after the seeds germinated, samples were taken from the roots of the plants to detect the added strain LH-ZY01 ( burkholderia sp. ) in the fixed value of crop roots and the existence of Rhizoctonia solani, the results are basically the same as those in Examples 1 and 2: LH-ZY01 ( burkholderia sp. ) strains existed in large numbers around the root system, and th...

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Abstract

The invention belongs to the field of the microorganisms and particularly relates to burkholderia LH-ZY01 (burkholderiasp.), and also relates to a strain and application thereof in suppressing rhizoctonia solani. The strain, namely the burkholderia LH-ZY01 (burkholderiasp.), is preserved in CCTCC (China Center For Type Culture Collection) on December 18, 2012; the preservation number of strain is CCTCCNO: M2012536. The burkholderia LH-ZY01 is combined with a loose nutrition carrier and the fermentation technology and the drying technology are adopted, so that the burkholderia is capable of growing in the nutrition carrier, and good in late-time stability and long in retention period. If the strain provided by the invention is applied to soil of crops infected by rhizoctonia solani such as rice, cotton, rape and tomato, diseases caused by the pathogenic bacteria can be remarkably eliminated; the efficiency of the strain for rice sheath blight disease is more than 75% and the efficiency for damping-off of colza is more than 59%; the diseases can be fundamentally eliminated from the soil to which the fertilizer is applied continuously for three crops.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to Burkholderia LH-ZY01 ( burkholderia sp. ), and also relate to the strain and its application in inhibiting Rhizoctonia solani. Background technique [0002] Rhizoctonia solani is a common pathogenic bacterium of many crops, and its infection range is very wide, not only involving various common vegetables, such as spinach, rape, tomato, cabbage, etc.; various common food crops such as rice, Wheat, etc.; there are apples, oranges, sugar cane, sweet oranges and other fruits. [0003] In addition, Rhizoctonia solani is also relatively stubborn in the soil and can generally survive in the soil for 3 years. It is the main cause of continuous sitting obstacles of cotton, rice and various vegetables. There are thousands of hectares of protected agricultural soil every year Due to continuous cropping obstacles, it is constantly abandoned, which causes a great waste of human, material and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F15/00C05F17/00A01N63/00A01P3/00C12R1/01
CPCY02W30/40
Inventor 黄道跟杨春玉赵建伟
Owner 山东鲁虹智慧农业研究院
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