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Bacillus amyloliquefaciens strain capable of resisting fruit tree pathogenic fungi in broad spectrum and application thereof

A technology for dissolving starch spores and bacilli, applied in the application, bacteria, fungicides and other directions, can solve the problems of less fungal diseases of fruit trees, pesticide residues harming human and animal health, polluting the environment, etc. simple conditional effect

Active Publication Date: 2016-03-02
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical fungicides are still mainly used to prevent and control fruit tree diseases. The advantages of chemical fungicides are fast action, good effect, and convenient use. They can eliminate or control the diseases that occur in a short period of time, and effectively reduce losses. It also makes mankind face new problems: a large number of pesticide residues endanger the health of humans and animals, and pollute the environment
At present, my country has successfully developed and put into production a variety of Bacillus commercial preparations, mainly to prevent fungal diseases such as sheath blight, powdery mildew, rice blast and other fungal diseases of grain and vegetables, but there are few biological preparations for fungal diseases of fruit trees

Method used

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  • Bacillus amyloliquefaciens strain capable of resisting fruit tree pathogenic fungi in broad spectrum and application thereof
  • Bacillus amyloliquefaciens strain capable of resisting fruit tree pathogenic fungi in broad spectrum and application thereof
  • Bacillus amyloliquefaciens strain capable of resisting fruit tree pathogenic fungi in broad spectrum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation and identification of Bacillus amyloliquefaciens YTB1407

[0026] The roots of American ginseng planted in Fushan District, Yantai City, Shandong Province, China, are cultivated and identified in the laboratory according to the following conditions, and can obtain the broad-spectrum antibacterial Bacillus amyloliquefaciens YTB1407 of the present invention:

[0027] Isolation and purification of YTB1407: The roots of American ginseng were surface sterilized, cut into small pieces, and cultured on NA solid medium. After 2 days, a single colony was picked, inoculated on NA solid medium by the partition streak method, cultured at 37°C for 1-2 days, and a single colony was picked and preserved.

[0028] The Bacillus amyloliquefaciens YTB1407 described in the present invention is identified based on its colony morphology, individual morphological characteristics, physiological and biochemical characteristics, phylogenetic tree of 16SrDNA and gyrA gene sequences, a...

Embodiment 2

[0035] Cultivation and fermentation conditions of the present invention

[0036] (1) Slope culture conditions: beef extract peptone solid medium: beef extract 3g, peptone 10g, NaCl 5g, agar powder 15g, water 1000mL, pH 7.0-8.0. 121℃, 0.1MPa steam sterilization for 20 minutes; culture temperature 37 °C for 2 to 4 days.

[0037] (2) Fermentation culture conditions: beef extract peptone liquid medium: beef extract 3g, peptone 10g, NaCl 5g, water 1000mL, pH 7.0-8.0. 121℃, 0.1MPa steam sterilization for 20 minutes; the culture was inoculated at 2% Inoculated into the fermentation medium, the liquid volume of the fermentation medium was 100 mL (the container was a 250 mL conical flask), the rotation speed was 150 rpm, the cultivation temperature was 30 °C, and the cultivation time was 4 days to obtain the fermentation broth.

[0038] The fermentation broth produced by the Bacillus amyloliquefaciens YTB1407 of the present invention is centrifuged at 5000 rpm for 10 minutes, and th...

Embodiment 3

[0040] Analysis of the antibacterial activity of broad-spectrum antibacterial thallus and fermentation broth produced by Bacillus amyloliquefaciens YTB1407 of the present invention

[0041] The indicator bacteria (apple rot, apple spot leaf leaf bacillus, grape white rot and grape anthracnose) were taken and placed in the middle of the potato solid medium plate with a diameter of 6 mm, and the antagonistic bacteria YTB1407 was inoculated around by the cross method. see attached Figure 4 . The following table shows the size of the inhibition zone of Bacillus amyloliquefaciens YTB1407 against different fruit tree phytopathogenic fungi:

[0042] Table 2 The size of the inhibition zone of Bacillus amyloliquefaciens YTB1407 against different fruit tree phytopathogenic fungi

[0043]

[0044] Take the sterile fermentation broth of Bacillus amyloliquefaciens YTB1407 of the present invention, add it to the sterilized potato solid medium at a ratio of 1:50, prepare a flat plate...

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Abstract

The invention relates to an antagonistic strain which is finally identified as Bacillus amyloliquefaciens through physiological and biochemical reaction, 16SrDNA and gyrA gene sequencing and sequence alignment; the Bacillus amyloliquefaciens is named Bacillus amyloliquefaciens YTB1407. The Bacillus amyloliquefaciens strain has antagonistic activity to various fruit tree pathogenic fungi, including macrophoma kawatsukai, ahernaria mali, grape anthracnose, coniothyrium diplodiella, plasmopara viticola and the like; the Bacillus amyloliquefaciens strain is antibacterial in broad spectrum, stable in anti-microbial activity and lasting in effect and has the huge potential for developing novel, efficient and natural biological control agents.

Description

technical field [0001] The present invention relates to Bacillus amyloliquefaciens strains Bacillus amylolique faciens YTB1407 also involves the indoor antagonistic activity and stability analysis of the strain against various fruit tree pathogenic fungi. Background technique [0002] Fruit tree disease is an important factor affecting the growth and fruiting process of fruit trees, which seriously restricts the yield and quality of fruit, and causes huge economic losses to the world every year. At present, chemical fungicides are still mainly used to prevent and control fruit tree diseases. The advantages of chemical fungicides are fast action, good effect, and convenient use. They can eliminate or control the diseases that occur in a short period of time, and effectively reduce losses. Human beings are faced with new problems: a large amount of pesticide residues endanger human and animal health and pollute the environment. Therefore, the development and utilization of n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 王英姿于晓丽李宝燕王培松
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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