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Inclusion compound for improving photostability of chromoprotein and application of inclusion compound

A pigment protein and photostability technology, applied in food science, cosmetic preparations, skin care preparations, etc., can solve problems such as precipitation and difficulty, and achieve the effects of reducing photodegradation, maintaining color and improving photostability

Active Publication Date: 2017-05-17
西安科艺诗生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to meet the shelf life of 2-3 years in the field of food, especially cosmetics, the existing technology has carried out experiments with more intense conditions. It was found that although polyphenols can reduce the photodegradation of phycocyanin, it is easy to cause phycocyanin solution The color changes from blue to green, and further, it will cause green precipitation
Therefore, it is still very difficult to have a stable blue color without changing the natural structure of phycocyanin

Method used

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  • Inclusion compound for improving photostability of chromoprotein and application of inclusion compound
  • Inclusion compound for improving photostability of chromoprotein and application of inclusion compound
  • Inclusion compound for improving photostability of chromoprotein and application of inclusion compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Polyphenol-cyclodextrin inclusion compound for improving photostability of phycocyanin

[0051] Weigh at least one polyphenol, add ethanol, and fully dissolve; weigh cyclodextrin, add water, stir to dissolve, place in a 40°C water bath, and add the ethanol solution of polyphenols to the cyclodextrin solution drop by drop while stirring , stirred at constant temperature for 4 hours, cooled to room temperature, stood still for 0.5 hours, filtered with suction, concentrated the filtrate to 100ml under reduced pressure, freeze-dried, and pulverized to obtain polyphenol-cyclodextrin inclusion compound; add appropriate amount of solid KBr to the inclusion compound, Thoroughly grind evenly and press into tablets, and test the infrared spectrum; the infrared spectrum of the inclusion compound shows that part of the spectral features of the contained polyphenols disappear, indicating that the polyphenol group has been in the cyclodextrin cavity, and the inclusion compo...

Embodiment 2

[0052] Example 2: Polyphenol-cyclodextrin inclusion compound for improving photostability of phycocyanin

[0053] Weigh at least one polyphenol, add ethanol, and fully dissolve; weigh cyclodextrin, add water, stir to dissolve, place in a water bath at 80°C, and add polyphenol ethanol solution to cyclodextrin solution drop by drop while stirring , stirred at constant temperature for 8 hours, cooled to room temperature, allowed to stand for 2 hours, suction filtered, and the filtrate was concentrated to 300ml under reduced pressure, freeze-dried, and pulverized to obtain polyphenol-cyclodextrin inclusion compound; inclusion compound was added with appropriate amount of solid KBr, fully Grind evenly and press into tablets, and test the infrared spectrum; the infrared spectrum of the inclusion compound shows that part of the spectral features of the contained polyphenols disappear, indicating that the polyphenol group has been in the cyclodextrin cavity, and the inclusion complex i...

Embodiment 3

[0054] Example 3: Resveratrol-hydroxypropyl-β-cyclodextrin inclusion compound and comparative test

[0055] 1. Experimental sample preparation

[0056] Weigh 10g of resveratrol, add 100ml of ethanol, and fully dissolve; weigh 100g of hydroxypropyl-β-cyclodextrin, add 400ml of water, stir to dissolve, and place in a 60°C water bath; while stirring, resveratrol Add the ethanol solution dropwise to the hydroxypropyl-β-cyclodextrin solution; stir at constant temperature for 6h. Cool to room temperature, let stand for 1h, and filter with suction. The filtrate was concentrated to 200ml under reduced pressure, freeze-dried and pulverized to obtain a resveratrol-hydroxypropyl-β-cyclodextrin inclusion complex with a resveratrol content of 10%.

[0057] Add an appropriate amount of solid KBr to the inclusion compound, grind it evenly and press it into tablets, and test the infrared spectrum; the infrared spectrum of the inclusion compound shows that part of the spectral features of th...

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Abstract

The invention particularly discloses an inclusion compound for improving the photostability of chromoprotein and application of the inclusion compound. The inclusion compound is prepared from an inclusion formed by at least one polyphenol molecule or partial groups of the polyphenol molecule in a cyclodextrin cavity; a polyphenol-cyclodextrin inclusion can improve the photostability of the chromoprotein, thereby helping the chromoprotein keep natural luster and activity; a protecting effect of the polyphenol-cyclodextrin inclusion is remarkably superior to a direction effect of polyphenol, and adverse influence of direct contact between the polyphenol and the chromoprotein can be reduced; the polyphenol-cyclodextrin inclusion can be mixed with the chromoprotein; sugar or sugar alcohol also can be added as an accessory in a mixing process and then a mixture is applied to a formula of food or cosmetics. According to the inclusion compound disclosed by the invention, pretreatment of the chromoprotein is avoided, and the structure of the chromoprotein is not changed, so that photodegradation of the chromoprotein is remarkably reduced, accurate luster of the chromoprotein also can be maintained, and important realistic significance for application of the chromoprotein in the cosmetics and the food is realized.

Description

technical field [0001] The invention mainly relates to the technical field of cosmetics and food, in particular, the invention relates to the technical field of improving the photostability of pigment protein. Background technique [0002] Due to its physiological activity, safety and natural color, natural active pigments have good application prospects in cosmetics, food, health care products and other fields. However, natural active pigments generally face the problem of poor stability, especially a type of pigment protein: that is, pigments composed of chromophores and protein subunits. Due to the combination with proteins, their stability is even worse. Phycocyanin and phycoerythrin are two typical pigment proteins. [0003] Phycocyanin mainly exists in cyanobacteria, red algae and cryptophyta, and it is one of the light-harvesting pigments for photosynthesis of these algae, especially in spirulina with a content as high as 10%-20% (Hao Wei, Pan Ling. Spiral Research ...

Claims

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Application Information

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IPC IPC(8): A61K8/73A61K8/34A61K8/60A61K8/49A61K8/37A61Q19/00A23L2/38A23L2/52
CPCA61Q19/00A23L2/38A23L2/52A61K8/347A61K8/375A61K8/498A61K8/602A61K8/738
Inventor 侯沛卢玲巧
Owner 西安科艺诗生物技术有限公司
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