55results about How to "High quality seedlings" patented technology

The present invention relates to the quick breeding method of high quality seedling of Phalaenopsis, known as 'queen orchid' and the culture medium therewith. The present invention uses the flower stem section of Phalaenopsis as explant and induces flower stem seedling with unique culture medium and performs proliferation with fascicular bud stem tip or stem section to produce high quality seedling of Phalaenopsis. The method is simple, practical, feasible and high in application value.

The invention relates to a pressure pulsation ultrasonic atomization culture device and cultural method using the same. The culture device of the invention comprises an atomization control system, and the atomization control system comprises an atomizing reactor culture-tank, an atomizing transducing chip arranged at the bottom of the culture-tank, an atomization generating device for controlling the atomizing transducing chip and a time relay, wherein the time relay is used for controlling the atomization generating device to start the atomizing transducing chip to atomize nutrient solution; and meanwhile, the culture device of the invention also comprises a pressure control system arranged on an intake pipe of the atomization control system, and the pressure control system comprises the time relay, an air pump, a gas-distributing tee, a gas flowmeter, an air filter and an electromagnetic valve, wherein the air after passing through the gas flowmeter is pumped into the gas-distributing tee by the time relay and the control pump, then the air enters the atomizing reactor culture-tank after passing through the air filter. The device and the method of the invention have the advantages of saving materials, reagents and manpower, shortening the breeding cycles of seedlings and having high production efficiency, therefore, the invention is easy to be used in large-scale production.

The invention provides a method for increasing the germination rate of papaya seeds. The method comprises the steps of firstly selecting good-quality papaya seeds, then soaking the selected good-quality papaya seeds for 0.5 minute by using water with the temperature being 55 DEG C before carrying out pretreatment and sowing, and then sowing. The method provided by the invention has the advantages that the germination rate of the papaya seeds is increased to 93% for the first time; buds and seedlings are neat, the quality of seedlings is high, the problem of low germination rate or long germination time of the papaya seeds under the conventional condition is solved; the method is an improvement of the key technology for breeding of the papaya and a simple and practical method is provided for mass propagation of papaya seedlings; and the application range is wide, and complex treatment procedures are not needed for each planter in the papaya planting region before sowing.

The invention relates to a light simplified seedling raising method for sweet potato true seeds. A diagonal nail clipper is used for cutting seed coats, a series of technical means of soil seedling raising, small plastic arch shed establishing, micro spray device laying, sunshade net covering, pest control net covering and the like are used, the problems that sweet potato true seed coats hard anddifficultly permeate and absorb oxygen, germination rate is low, seedling rate is low, weak seedlings is more and the quality is poor are solved, and the unification of sweet potato true seed seedlingraising light simplification, the high germination rate, the high emergence rate and the strong seedling rate is achieved. By adopting the method, compared with other methods, the operation is simpler and safer, and the method is lower in cost and higher in seedling germination rate and emergence rate, is more capable of obtaining strong seedlings, and provides protection for breeding of novel sweet potato varieties.

The invention relates to the technical field of detoxification and propagation of fruit trees, and in particular relates to a method for rapid propagation and detoxifying of stem tips of fragrant pomelo, and the method comprises the following steps: (1) selection and disinfection of explants, to be more specific, taking a fragrant pomelo tuber in spring or autumn for indoor germination, spraying regularly a bactericide after buds grow up, two weeks later, taking the buds again, after heat treatment in a 5% sodium hypochlorite solution of the buds for 8-10 minutes at 35-40 DEG C, putting the buds on a super clean bench under an anatomical lens for stem tip exfoliation under 8-40 times, and selecting the stem tip or a nucellar tissue of an apical bud for standby use; and (2) primary culture,to be more specific, putting the selected stem tip or the nucellar tissue once again in the 5% sodium hypochlorite solution for treating for 3-5min, and putting the stem tip or the nucellar tissue into a primary medium for rapid propagation and growth. The method for rapid propagation and detoxifying of the stem tips of the fragrant pomelo is established by selection of mediums and hormones in each stage of sterilization and tissue culture of the external explants, provides a theoretical basis and a technical support for factory production of fragrant pomelo tissue culture seedlings, and provides a new way for selection and breeding of new varieties.

The invention discloses a high-quality and high-yield cultivation method for bletilla striata seedlings. The method comprises the following steps of (1) tissue culture, specifically, primary culture, multiplication culture and rooting and seedling strengthening culture; (2) hardening the tissue culture seedlings, specifically, treating a matrix, selecting bottle seedlings, hardening the seedlings in a bottle and hardening the seedlings in a seedbed; and (3) field planting. Bletilla striata seeds serve as explants, tissue culture seedlings are cultured, different culture media are replaced in different periods to adapt to the growth condition of bletilla striata, the pollution rate of bletilla striata is reduced, the cost is reduced, meanwhile, the quality and yield of bletilla striata seedlings are improved, and comprehensive popularization and research can be carried out.

The invention belongs to the technical field of plant tissue culture and particularly relates to a method for in-bottle rooting of prunus glandulosa tissue culture seedlings. The method comprises selection of subculture seedlings and culture by a rooting culture medium, wherein standards for selection of the subculture seedlings are as follows: the single-plant height is 3cm to 4cm, the growth isrobust, and leaves are unfolded; and ingredients of the rooting culture medium comprise 0.4mg / L-0.8mg / L of 1 / 2WPM+IBA. According to the method, a culture medium formula with relatively high rooting rate is employed, the rooting rate of in-bottle rooting of the prunus glandulosa tissue culture seedlings is effectively increased and can reach 95%-100%, the nondefective rate of the prunus glandulosatissue culture seedlings is high, a tissue culture method is easy to operate, the production cost can be reduced greatly, the speed of propagation and the uniformity of the seedlings are improved, thetrait stability is improved, the factory production of prunus glandulosa is accelerated, and high-quality prunus glandulosa seedlings are provided for the market.

A rooting method for tissue-cultured shoots of cinnamomum camphora (L.) presl is disclosed. The method includes selecting cinnamomum camphora (L.) presl subcultured shoots after 35-40 d strong-shoot culture in conventional tissue culture, cutting single shoots robust in growth and having height not less than 2 cm from the subcultured shoots, inserting into an ascorbic acid sterile solution, soaking for pretreatment, vertically inserting the pretreated single shoots into a rooting culture medium, performing rooting culture in a specific light-temperature environment, conveying to a seedling growing house under natural environment conditions, and performing seedling hardening to obtain seedlings of the cinnamomum camphora (L.) presl. Through pretreating the subcultured shoots, the sassafras oil content in a notch is reduced, the rooting period is shortened, the rooting percentage is high, quality is good, and the survival rate of transplanting of the tissue-cultured rooted seedlings is high. Industrialized tissue-cultured seedling breeding can be achieved. High-quality seedlings can be provided for construction of a natural perfume artificial clonal plantation. The method has good economic benefit, ecological benefit and social benefit.

The invention discloses an introduction and cultivation technique of pigment marigold in a high altitude area, in particular an introduction and cultivation technique for an area at an altitude between 1,700 and 2,200 meters. The method comprises the steps of pretreatment of seeds of pigment marigold, seedling raising, transplanting, management and harvesting. The method adopts physical and chemical method to pretreat the seeds to achieve a germination percentage of the seeds of 90 percent and a uniform seedling raising means to provide high quality seedlings for large scale planting. By planting pigment marigold in a high altitude area, the yield of fresh flowers and content of xanthophylls in the fresh flower are greatly improved. The cultivation method, compared with the prior method, contributes to construction of standard pigment marigold plantations, carrying out standard planting demonstration of pigment marigold and improving the technological content of the pigment marigold, enables farmers to learn planting technologies of pigment marigold as quickly as possible and obtain high quality pigment marigold products, and greatly improves economic benefits.

The invention discloses a group of rapid marsdenia tenacissima propagation culture media. The main formula of the group of culture media is as follows: an induction medium of MS+6-BA 2.0mg / L+NAA 0.5mg / L; an enrichment medium of MS+6-BA 1.0mg / L+NAA 0.3mg / L; a sound seedling culture medium of MS+6-BA 1.0mg / L+NAA 0.3mg / L+NH4NO3 0.66g / L+NaH2PO4 0.56g / L+K2SO4 1.78g / L+banana 100g / L; and a rooting medium of 1 / 2MS+6-BA 0.5mg / L+IAA 0.2mg / L. The induction rate is over 88 percent, the proliferation rate is high, the rooting rate is over 95 percent, the tissue culture propagation period is short and is only 64-72 days, and effective and rapid vegetative propagation of the marsdenia tenacissima is realized.

The invention discloses a strain containing bacillus dead valley and the functional vegetable seedling-raising biological substrate prepared therefrom. A Bacillus dead valley N23 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO.9920. The functional vegetable seedling-raising biological matrix is ​​obtained by adding functional bacterial strain N23 to common seedling-raising matrix. This product is aimed at cultivating seedlings of watermelon, pepper, tomato, cucumber and other crops. Select a specific Bacillus dead valley N23, and add it to the matrix to obtain an active biological matrix product. Using this product, it can be used for watermelon, pepper, tomato, etc. , the growth-promoting effect of cucumber seedlings is good, and high-quality seedlings with a large number of active functional microorganisms in the rhizosphere can be bred. It can also improve the yield of such crops in field planting.

The invention discloses an imitative-ecological podophyllum hexandrum seedling raising method, belongs to the field of ecological planting of medicinal plants and aims at solving the problems of low seedling raising rate, slow seedling growth, long seedling raising period, high cost and incidence rate increase of root rot, damping off and other diseases at a seedling stage of an existing method. The method comprises the steps of seed harvesting and storage, seed germination accelerating, sowing in a greenhouse holed tray, young seedling growth promotion and prevention and control of the diseases at the seedling stage, early sowing, planting and transplantation of podophyllum hexandrum holed-tray seedlings and culture and harvesting of high-quality podophyllum hexandrum seedlings. By adopting the imitative-ecological podophyllum hexandrum seedling raising method, the most suitable environment and nutrition conditions are created at different growth and development stages, the physiological conditions of the seedlings are improved, the seedling raising rate of the method is improved, seedling raising time is shortened, high-quality strong seedlings are cultured, further the reviving rate in a next year is improved, growth and development of the seedlings are promoted, the incidence rate is reduced, the cost is reduced, and the productivity is improved through intensive measures to achieve the best seedling raising effect.

The invention discloses a functional vegetable seedling-raising biological matrix containing Bacillus subtilis G10 and a preparation method thereof. A strain of Bacillus subtilis (Bacillus subtilis) G10 was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, with the preservation date being November 3, 2014, and the preservation number is CGMCC NO.9921. A functional vegetable seedling-raising biological substrate is obtained by adding the Bacillus subtilis G10 fermentation liquid of the present invention to a common seedling-raising substrate. The use of this functional biological substrate for vegetable seedlings has a good effect on promoting the growth of vegetables and fruits at the seedling stage, and high-quality seedlings can be obtained. growth.

A pteris wallichiana tissue culture rapid breeding method comprises the following steps: (1) taking pteris wallichiana mature spores as explants for disinfection; (2) putting the disinfected explants into a MS basic culture medium for inducing prothallium; (3) putting the prothallium into the MS basic culture medium for strong seedling culture to obtain sporozoites differentiated into plantlets; (4) putting the sporozoites differentiated into plantlets in a 1 / 2MS rooting culture medium for culture to obtain complete rooted seedlings; and (5) taking the complete rooted seedlings for hardening and then transplanting to a nursery greenhouse. Plants obtained by the method are strong and thick and high in survival rate, a lot of high-quality pteris wallichiana seedlings suitable for cultivation are provided, and the pteris wallichiana scale breeding problem can be effectively solved.