Dendrobium officinalis anaerobic fermentation residue culture medium and preparation method thereof

Abstract

The invention relates to a dendrobium officinalis anaerobic fermentation residue culture medium and a preparation method thereof and belongs to the technical field of agricultural biotechnology. The anaerobic fermentation residue contains abundant nitrogen, phosphorus, potassium, calcium, copper, iron, magnesium, manganese, zinc and such nutritional elements, and amino acids, hydrolases, B-group vitamins, hormones and the like. The culture medium prepared from the anaerobic fermentation residue is quite suitable for growth of dendrobium officinalis. The culture medium can be used for quickly obtaining high-quality seedlings of the dendrobium officinalis. The culture medium is simple in formula, is low in cost, is quick in growth of the seedlings of the dendrobium officinalis, and is good in growth and stress resistance of the dendrobium officinalis. The culture medium not only improves utilization capability of biogas mature but also reduces tissue culture cost, thereby promoting popularization of rapid seedling cultivation of the dendrobium officinalis.

Description

technical field [0001] The invention relates to a culture medium for anaerobic fermentation residue of dendrobium officinale and a preparation method thereof, belonging to the field of agricultural biotechnology. Background technique [0002] Dendrobium officinale (Dendrobium candidum Wall. Ex Lindl. ） Also known as black knot grass, it is a perennial epiphytic herb of the genus Dendrobium orchidaceae. The stem is erect, cylindrical, and unbranched; the long stem is slightly curved when it blooms, and the leaves are born on the upper node of the stem, two rows, oblong shape It is lanceolate, and the inflorescence is born on the upper node of the stem without leaves. It is a precious and endangered medicinal material under national second-level protection. It is regarded as "the top grade of medicine" in medical classics of all dynasties. The efficacy of the lungs. Due to the limitation of special biological and ecological characteristics, its natural fecundity is extremel...

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Problems solved by technology

[0004] In order to solve the above-mentioned defects existing in the prior art, the object of the present invention is to provide a kind of anaerobic fermentation residue culture of Dendrobium candidum for the problems of high cost of tissue culture of Dendrobium candidum, poor stress resistance of tissue culture seedlings, and low survival rate of transplanting. Base, the anaerobic fermentation residue medium of Dendrobium officinale is composed of the following raw materials in mass percentage: 5-30% of anaerobic fermentation residue, 10-40% of MS basic medium, 10-40% of 1 / 2MS basic culture , sucrose 2-3%, banana 5-10%, potato 5-10%, agar powder 0.35-0.5%, auxin 1.5×10 -5 ~1×10 -4 %, Cytokinin 2×10 -5 ~2×10 -4 %, activated carbon 0.2~0.4%, the balance is distilled water