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Method for in-bottle rooting of prunus glandulosa tissue culture seedlings

A technology of tissue culture seedlings and wheat plums, applied in the field of plant tissue culture, can solve the problems of few high-quality commercial seedlings and low survival rate, achieve high rooting rate and good product rate, improve the stability of traits, increase the speed of reproduction and the growth rate of seedlings The effect of uniformity

Active Publication Date: 2020-05-01
WUXI MISHO ECOLOGY LANDSCAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is commonly used for propagation by division or grafting. The rootstock is mountain peach, and cuttings are used for propagation. In summer, semi-mature branches are easy to survive, but the survival rate is not high, and there are few high-quality commercial seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for rooting wheat plum tissue culture seedlings in a bottle, comprising the following steps:

[0014] (1) Selection of offspring seedlings: divide the offspring seedlings into individual plants, separate the height from the short ones, and select the offspring wheat and plum seedlings with a height of 3-4cm per plant, strong growth and stretched leaves;

[0015] (2) Rooting medium culture: transfer the selected secondary seedlings to the rooting medium 1 / 2WPM+IBA 0.4mg / L, the seedlings grow root primordia in 7 days, the roots are 3~4cm long in 10 days, and the rooting rate is 95%. %.

[0016] The rooting medium also includes 26 g / L sucrose, 4 g / L agar powder, the pH of the medium is 5.8, the culture temperature is 22° C., the light time is 8 hours per day, and the light intensity is 1500 lx.

Embodiment 2

[0018] A method for rooting wheat plum tissue culture seedlings in a bottle, comprising the following steps:

[0019] (1) Selection of offspring seedlings: divide the offspring seedlings into individual plants, separate the height from the short ones, and select the offspring wheat and plum seedlings with a height of 3-4cm per plant, strong growth and stretched leaves;

[0020] (2) Rooting medium culture: transfer the selected secondary seedlings to the rooting medium 1 / 2WPM+IBA 0.5mg / L, the seedlings grow root primordium in 8 days, the roots are 3~4cm long in 12 days, and the rooting rate is 97%. %.

[0021] The rooting medium also includes 27 g / L sucrose, 4.5 g / L agar powder, the pH of the medium is 5.9, the culture temperature is 23° C., the light time is 9 hours per day, and the light intensity is 2000 lx.

Embodiment 3

[0023] A method for rooting wheat plum tissue culture seedlings in a bottle, comprising the following steps:

[0024] (1) Selection of offspring seedlings: divide the offspring seedlings into individual plants, separate the height from the short ones, and select the offspring wheat and plum seedlings with a height of 3-4cm per plant, strong growth and stretched leaves;

[0025] (2) Rooting medium culture: transfer the selected secondary seedlings to the rooting medium 1 / 2WPM+IBA 0.6mg / L, the seedlings grow root primordia in 10 days, the roots are 3~4cm long in 12 days, and the rooting rate is 98%. %.

[0026] The rooting medium also includes 28 g / L sucrose, 5 g / L agar powder, the pH of the medium is 6.0, the culture temperature is 24° C., the light time is 10 hours per day, and the light intensity is 2500 lx.

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Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a method for in-bottle rooting of prunus glandulosa tissue culture seedlings. The method comprises selection of subculture seedlings and culture by a rooting culture medium, wherein standards for selection of the subculture seedlings are as follows: the single-plant height is 3cm to 4cm, the growth isrobust, and leaves are unfolded; and ingredients of the rooting culture medium comprise 0.4mg / L-0.8mg / L of 1 / 2WPM+IBA. According to the method, a culture medium formula with relatively high rooting rate is employed, the rooting rate of in-bottle rooting of the prunus glandulosa tissue culture seedlings is effectively increased and can reach 95%-100%, the nondefective rate of the prunus glandulosatissue culture seedlings is high, a tissue culture method is easy to operate, the production cost can be reduced greatly, the speed of propagation and the uniformity of the seedlings are improved, thetrait stability is improved, the factory production of prunus glandulosa is accelerated, and high-quality prunus glandulosa seedlings are provided for the market.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rooting wheat plum tissue culture seedlings in a bottle. [0002] technical background [0003] Mai Li, Rosaceae Prunus, deciduous shrub, up to 2 meters, leaves ovate-elliptic to elliptic-lanceolate, 5-8 cm long, apex acute and often rounded, base broadly cuneate, margins thin and blunt Teeth, glabrous on both sides or sparsely pubescent on the back of the middle rib; petiole 4-6mm long. The flowers are pink or nearly white, about 2 cm in diameter, and the pedicel is about 1 cm long. Fruit nearly spherical, 1-1.5 cm in diameter, red. Mai Li has a certain degree of cold resistance, strong adaptability, light-loving, relatively cold-resistant, strong adaptability, and is very beautiful. It is commonly cultivated and ornamental in gardens everywhere. It is suitable for lawn, roadside, rockery and forest edge cluster planting. Flower forcing an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周敬锋田星凯李涛
Owner WUXI MISHO ECOLOGY LANDSCAPE
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