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Pteris wallichiana tissue culture rapid breeding method

A technology of tissue culture and Pteris fern, applied in gardening methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of unqualified breeding technology, destruction of wild fern resources, long time required, etc., and achieve reduction Effects of cultivation steps, shortening of cultivation time, and reduction of cultivation cost

Inactive Publication Date: 2016-03-09
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional propagation methods of ferns mainly rely on spore propagation and branch propagation, and the seedling rate of spore propagation in the natural environment is very low, and branch propagation takes a long time and the number of seedlings obtained is small, which cannot satisfy a large number of plants in a short period of time. seedling needs
At present, due to the unqualified breeding technology, it is mainly obtained through field excavation, and field excavation has caused serious damage to wild fern resources. The interference of human activities and the causes of the plants themselves have threatened fern resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An example of the Southwestern Pteris fern tissue culture rapid propagation method of the present invention, comprises the steps:

[0020] (1) Selection and disinfection of explants: take the mature spores of Pteris chinensis as explants, rinse the explants slowly under running water, remove the dirt on the surface, put them in a beaker, and use 2v / v% After soaking in the cleansing liquid for 15 minutes, rinse the explants with running water for 10 minutes, move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then add 1-2 drops of Tween-20 100ml concentration is 0.1v / v% HgC1 2 Disinfect for 8 to 15 minutes, soak in sterile water for 3 times, use sterile filter paper to dry the surface of the explants before inoculating, wherein the sterile water is distilled water sterilized by high temperature and high pressure;

[0021] (2) Spore induction: Inoculate the explants obtained in step (1) into the MS induction medium at a ...

Embodiment 2

[0026] An example of the Southwestern Pteris fern tissue culture rapid propagation method of the present invention, comprises the steps:

[0027] (1) Selection and disinfection of explants: take the mature spores of Pteris chinensis as explants, rinse the explants slowly under running water, remove the dirt on the surface, put them in a beaker, and use 2v / v% After soaking in the cleansing liquid for 15 minutes, rinse the explants with running water for 10 minutes, move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then add 1-2 drops of Tween-20 100ml concentration is 0.1v / v% HgC1 2 Disinfect for 8 to 15 minutes, soak in sterile water for 3 times, use sterile filter paper to dry the surface of the explants before inoculating, wherein the sterile water is distilled water sterilized by high temperature and high pressure;

[0028] (2) Spore induction: Inoculate the explants obtained in step (1) into the MS induction medium at a ...

Embodiment 3

[0033] An example of the Southwestern Pteris fern tissue culture rapid propagation method of the present invention, comprises the steps:

[0034] (1) Selection and disinfection of explants: take the mature spores of Pteris chinensis as explants, rinse the explants slowly under running water, remove the dirt on the surface, put them in a beaker, and use 2v / v% After soaking in the cleansing liquid for 15 minutes, rinse the explants with running water for 10 minutes, move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then add 1-2 drops of Tween-20 100ml concentration is 0.1v / v% HgC1 2 Disinfect for 8 to 15 minutes, soak in sterile water for 3 times, use sterile filter paper to dry the surface of the explants before inoculating, wherein the sterile water is distilled water sterilized by high temperature and high pressure;

[0035] (2) Spore induction: Inoculate the explants obtained in step (1) into the MS induction medium at a ...

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Abstract

A pteris wallichiana tissue culture rapid breeding method comprises the following steps: (1) taking pteris wallichiana mature spores as explants for disinfection; (2) putting the disinfected explants into a MS basic culture medium for inducing prothallium; (3) putting the prothallium into the MS basic culture medium for strong seedling culture to obtain sporozoites differentiated into plantlets; (4) putting the sporozoites differentiated into plantlets in a 1 / 2MS rooting culture medium for culture to obtain complete rooted seedlings; and (5) taking the complete rooted seedlings for hardening and then transplanting to a nursery greenhouse. Plants obtained by the method are strong and thick and high in survival rate, a lot of high-quality pteris wallichiana seedlings suitable for cultivation are provided, and the pteris wallichiana scale breeding problem can be effectively solved.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant tissue culture, in particular to a method for rapid propagation of Southwest phoenix tail tissue culture. Background technique [0002] Pteris wallichiana is a large terrestrial plant of the genus Pteris wallichiana in the Pteridaceae family. It grows in forest valleys or forest margins at an altitude of 800-2000m. Distributed in Taiwan, Guangdong, Guangxi, Guizhou, Hainan and other places. Bitter, astringent, cool in nature. It has the functions of clearing away heat and stopping dysentery, relieving convulsion, and stopping bleeding. Indications of dysentery, convulsions in children, traumatic bleeding embolism. [0003] Southwestern Pteris fern has beautiful leaf shape and green leaf color, which has high garden ornamental value and certain medicinal value. Medicinal ferns contain various active substances such as alkaloids, flavonoids, and phenols, which have obvious therapeutic e...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008Y02A40/10
Inventor 王一诺韦坤华韦莹李翠李林轩李磊肖冬
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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