Quick breeding method for butterfly orchid high quality sprout
A Phalaenopsis, high-quality technology, applied in the field of rapid propagation of high-quality Phalaenopsis seedlings, can solve the problems of rapid propagation and large-scale production of high-quality Phalaenopsis seedlings that have not existed before, and achieve simple and affordable technology, high genetic stability, and rapid emergence Effect
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[0015] 1. Material: Phalaenopsis Taida Salu (Phalaenopsis Taida Salu "Sunset Red")
[0016] 2. Disinfection of explants: take the pedicel of the safflower bud, rinse it with tap water, cut off the segment with dormant buds 2-3 cm, soak it with 70% alcohol on the ultra-clean workbench for 1 minute, Then sterilize with 0.1% (weight percent) mercuric chloride solution for 15 minutes, rinse with sterile water for 5 times, blot dry with sterile absorbent paper, cut off 0.5 cm at both ends of the incision, and cut into 1-2 cm long with dormant buds Cut sections were inoculated on pedicel seedling induction medium.
[0017] 3. Induction of pedicel seedlings: the induction medium of pedicel seedlings is VW+6-benzylpurine 10 mg / liter+naphthalene acetic acid 0.5 mg / liter+sodium citrate 150 mg / liter, 15-day dormant buds start to grow, and in culture When the temperature is (28±2)℃, pedicel seedlings with leaves are mostly formed.
[0018] 4. Proliferation of clustered buds: when cultur...
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