Separation purification process and application of anaerobic degradation pure culture of polycyclic aromatic hydrocarbon

Abstract

The invention discloses a separated purification method and the application thereof of a polycyclic aromatic hydrocarbon anaerobic degrading pure strain, which belongs to the polluted soil and aqueous biological degradation handling field. A base bottom layer flat plate is manufactured in the a anaerobic box filled with nitrogen gas by taking polycyclic aromatic hydrocarbon as a sole carbon source and an energy source and taking sodium nitrate or potassium nitrate as an electronic accepted anaerobic agar solid culture, the top layer flat plate of the anaerobic agar solid culture including polycyclic aromatic hydrocarbon anaerobic degrading mixed bacteria is manufactured on the bottom layer flat plate, the manufactured culture medium flat plate is put in a drying container filled with the nitrogen gas to culture one to two months after being sealed. Polycyclic aromatic hydrocarbon, electronic acceptance, basic culture medium, trace metal liquid, vitamin c solution and colony in the culture medium flat plate connected into the anaerobic bottle of resazurin are added in the anaerobic box filled with the nitrogen gas, and are cultured for 20 to 25 days in an oscillator under the temperature of 20 plus or minus 2 DEG C and the rotating speed of 100 r / min. Then, the cyclical switchover is performed, after a cycle time is larger than four times, the pure strain of the anaerobic degrading polycyclic aromatic hydrocarbon can be obtained. The method can obtain good microbe pure strain to the polycyclic aromatic hydrocarbon anaerobic degrading performance by being separated.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and in particular relates to a separation and purification method and application of polycyclic aromatic hydrocarbon anaerobic degradation pure bacterial species. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of organic compounds with carcinogenic, teratogenic and mutagenic effects. They are widely distributed in the environment and have biodegradability, which has attracted widespread attention. The aerobic biodegradation of polycyclic aromatic hydrocarbons is the fastest, and many strains of aerobic degradation bacteria have been isolated and domesticated. However, underground aquifers or polluted soils are usually in an anaerobic state, so that aerobic microorganisms in the contaminated zone are replaced by anaerobic microorganisms. Therefore, anaerobic biodegradation of PAHs is more feasible and cost-effective tha...

AI Technical Summary

Problems solved by technology

[0003] So far, there are no domestic literature and patent reports on the methods and technologies for the separation and purification of polycyclic aromatic hydrocarbons anaerobic degradation bacteria

At the same time, because polycyclic aromatic hydrocarbons, especially fused-ring aromatic hydrocarbons highe...