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Bactericide for preventing and curing crop bacterial diseasess as well as preparation method and application thereof

A technology of bacteria and crops, which is applied in the field of bacterial agents for preventing and treating bacterial diseases of crops and its preparation, can solve problems such as effective methods for bacterial diseases of Bdellovibrio crops that have not yet been seen, and achieve excellent elimination ability, fast reproduction, and safety of natural enemies Effect

Inactive Publication Date: 2010-02-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, Bdellovibrio has been developed to eliminate pathogenic bacteria in aquaculture products, but there is no effective method for Bdellovibrio to prevent and control bacterial diseases of crops

Method used

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  • Bactericide for preventing and curing crop bacterial diseasess as well as preparation method and application thereof
  • Bactericide for preventing and curing crop bacterial diseasess as well as preparation method and application thereof
  • Bactericide for preventing and curing crop bacterial diseasess as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 prevents and treats rice bacterial blight

[0040] (1) Preparation of Bdellovibrio bacterial liquid

[0041]The fermentation method of the Bdellovibrio liquid adopts the fermentation method of the high-density Bdellovibrio swimming body disclosed in the application number "200710031166.X", and the title is "Fermentation and Culture Technology of High-density Bdellovibrio Swimming Body" National Invention Patent Application: respectively Inoculate Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Institute of Microbiology, No. GIM1.172) in 3 Erlenmeyer flasks equipped with 100mL LB (Luria-Bertani) liquid medium, 200rpm, Cultivate on a shaker at 28°C for 18 hours, centrifuge the culture solution at 4°C and 8000rpm for 10min, and discard the supernatant. Add the precipitates to 3 containers containing 100mL DNB liquid medium (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, NaCl 30g, di...

Embodiment 2

[0053] Embodiment 2 prevents and treats rice stripe disease

[0054] (1) The preparation of Bdellovibrio bacterial liquid is the same as step (1) of Example 1.

[0055] (2) Preparation of bacterial agents for preventing and treating bacterial diseases of crops

[0056] Bdellovibrio BDS01, Bdellovibrio BDS02 and Bdellovibrio BDSM08 were mixed according to the number of bacteria 1:1 in pairs (that is, BDS01 and BDS02, BDS01 and BDSM08, BDS02 and BDSM08), and added to each liter of the mixed bacteria liquid 15g agar oligosaccharides and 15g dextran oligosaccharides were shaken for 30s with a magnetic stirrer to obtain bacterial agents A, B and C for preventing and treating bacterial diseases of crops;

[0057] Mix Bdellovibrio BDS01, Bdellovibrio BDS02 and Bdellovibrio BDSM08 according to the number of bacteria 1:1:1, add 15g agarose oligosaccharide and 15g dextran oligosaccharide to each liter of mixed bacteria solution, shake with a magnetic stirrer 30s, get bacterial agent D...

Embodiment 3

[0061] Embodiment 3 prevents and treats bacterial angular leaf spot of cucumber

[0062] (1) The preparation of Bdellovibrio bacterial liquid is the same as step (1) of Example 1.

[0063] (2) Preparation of bacterial agents for preventing and treating bacterial diseases of crops

[0064] Mix the Bdellovibrio BDS01 and Bdellovibrio BDSM08 bacteria solutions according to the number of bacteria 1:1, dilute with normal saline, the concentration is 10 3 pfu / mL, 10 5 pfu / mL, 10 7 pfu / mL and 10 9 There are four concentration levels of pfu / mL, then add 10g chitosan oligosaccharide and 20g dextran oligosaccharide to each liter of mixed bacterial solution in different concentrations of Bdellovibrio solutions, and shake it with a magnetic stirrer for 30s to obtain the control of bacterial diseases of crops The bacterial agents A, B, C and D of Bdellovibrio BDS01, BDS02, and BDSM08 were mixed according to the number of bacteria 1:1:1, diluted with normal saline, and the concentration...

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Abstract

The invention discloses a bactericide for preventing and curing crop bacterial diseasess as well as a preparation method and application thereof. In the preparation method, oligosaccharide is added into bdellovibrio bacteriovorus solution to be evenly mixed to obtain the bactericide for preventing and curing crop bacterial diseasess. The invention applies bdellovibrio bacteriovorus solution for preventing and curing crop bacterial diseases for the first time, fills the vacancy of researching and developing bdellovibrio bacteriovorus at home and abroad and adds new species for biopesticide. Asbdellovibrio bacteriovorus has the characteristics of broad cracking pattern and quick growth and has the outstanding capability of eliminating various pathogenicbacteria and bacteria, after combinedwith oligosaccharide, the bdellovibrio bacteriovorus strengthens crop resistance, greatly improves pesticide effect and enlarges the preventing and curing spectrum of bacterial diseases. The bactericide prepared by the invention has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, pathogenic bacteria have small possibility of producing resistance, so that the invention overcomes 3R problems brought by abusing antibiotics and avoids the defects of serious chemical pesticide pollution and ecological balance damage at present.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a fungal agent for preventing and treating bacterial diseases of crops, a preparation method and application thereof. Background technique [0002] In recent years, my country's agriculture has developed rapidly. With the improvement of intensive planting, some common bacterial diseases have become more prevalent and stubborn. The effective methods in the past are not effective now, and even the so-called sensitive drugs in the past are ineffective. . Bacterial diseases are diseases caused by bacterial infection, such as rice bacterial blight, soft rot, canker, bacterial wilt, etc., which have caused significant losses in agricultural production. In particular, rice bacterial blight is one of the three major diseases of rice. In my country, the annual occurrence area is more than 10 million mu, and the yield is generally reduced by 10% to 20%. In severe cases, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N43/16A01P1/00C12N1/20C12R1/63
Inventor 蔡俊鹏韩晓宁
Owner SOUTH CHINA UNIV OF TECH
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