57 results about "Coliphage" patented technology

The invention belongs to the technical field of escherichia coli phage, and particularly relates to a broad-spectrum escherichia coli phage capable of cracking four bacteria and an application of the broad-spectrum escherichia coli phage to sterilization and bacterium prevention. The invention mainly discloses an escherichia coli phage EC35P1 (Escherichia coli phase EC35P1), and the preservation number of the escherichia coli phage EC35P1 is CCTCC M 2020438. The phage is a virulent phage separated from the nature, tests prove that the phage has no toxic effect on normal microbial flora, and DNA of the phage cannot encode virulence genes and is high in stability. The phage is wide in host range, can be used for cracking escherichia coli, shigella, salmonella and enterobacter cloacae, and large-scale industrial production can be realized. The phage provides an excellent strain resource for developing a novel antibacterial preparation, and has a good application and development prospect.

The invention belongs to the technical field of Escherichia coli, and particularly relates to a wide-lysis-spectrum Escherichia coli phage and a composition, a kit and application thereof. The phage is Escherichia coli phage CL1 which is collected under the number of CCTCC M 2018936. The phage is nonhazardous to normal microbial flora, resistant to high temperature and high in stability and has good application and development prospect, DNA of the phage cannot code virulence genes, and an excellent strain resource is provided for developing novel antibacterial preparations.

The invention discloses a high-temperature-resistant Escherichia coli phage RDP-EC-20031 with the preservation number of CGMCC No.21406 and the gene length of 135812bp, which is discovered to have no virulence gene and lysogenic gene after sequencing the whole gene, and the gene of the phage contains a plurality of genes of lytic enzyme. The Escherichia coli phage RDP-EC-20031 provided by the invention has higher temperature tolerance and wider acid-base tolerance range, and is beneficial to industrial production and preparation; and the phage is found to have a lysis rate of 98% by lysis rate test and RTD test, and whenthe phage diluted by 107 times,the phage still has a good cracking effect on pathogenic bacteria. The phage has the characteristics of excellent performance and safety. The phage can be applied to preparation of medicines for preventing and / or treating diseases caused by escherichia coli.

The invention discloses an escherichia coli bacteriophage vBE_coM_swi3 with a preservation number of CCTCC NO: M2019467. The bacteriophage belongs to myoviridae and has a polyhedral head and a telescopic tail structure, the diameter of the head is about 80 nm, and the length of the tail is about 120 nm; transparent empty spots can be formed on a solid culture medium, no halo ring exists around thesolid culture medium, the edge is clear and regular, and the diameter is about 1-1.5 mm; the bacteriophage has a good cracking effect on swine-derived and chicken-derived escherichia coli in a breeding environment, particularly on swine-derived and chicken-derived pathogenic escherichia coli, and has very wide application prospects in multiple aspects of pharmaceutical preparations, feed additives and the like for preventing and treating swine colibacillosis and chicken colibacillosis.

The invention discloses a phage flocculant and application of the phage flocculant in a fermentation post-treatment technology and belongs to the field of a microbial post-treatment technology. The phage flocculant is characterized in that the phage flocculant is a cationic flocculant, and a working concentration of the phage flocculant is 0.1-5% generally. The flocculant is applied in the fermentation post-treatment technology of a vibrio parahaemolyticus phage, a coliphage, a salmonella phage, a xanthomonas axonopodis phage, a ralstonia solanacearum phage, a staphylococcus aureus phage, a streptococcus agalactiae phage, a streptococcus iniae phage or a pseudomonas aeruginosa phage. The phage flocculant can play a role of rapid subsidence of bacteria, be convenient and quick to operate, reduce centrifugal equipment investment and manpower and material resource loss and meet requirements of industrial mass production of the phage, and is safe, nontoxic and harmless to animals and plants.

The invention discloses a test and quality control kit and test method of Hepatitis A virus and Norovirus in a vegetable. The kit comprises one tube of HAV 2*RT-PCR mix, NV GI+GII parting 2*RT-PCR mix, MS2 2*RT-PCR mix, RNase free water, HAV positive control, GI+GII positive control and coliphage MS2 (2.5*1010 pfu / mL) respectively. The test method comprises the following steps: (1) enrichment of virus in a vegetable sample; (2) extraction of virus RNA; (3) establishment of a coliphage MS2 standard curve; (4) test of Hepatitis A virus, Norovirus GI type and GII type and coliphage MS2; (5) determination of the recovery rate of virus RNA; (6) determination of results. According to the test and quality control kit and test method of the Hepatitis A virus and Norovirus in the vegetable, the quality is effectively guaranteed in the whole virus detection process of critical steps of sample pretreatment, virus concentration, quality control, test, result determination and the like, stability, accuracy and controllability of the test process are enhanced, and the detection technology blank in the field is filled.

The invention belongs to the technical field of Escherichia coli, and particularly relates to a high-temperature-resistant Escherichia coli phage and a composition, a kit and application thereof. Thephage is Escherichia coli phage CL9 which is collected under the number of CCTCC M 2018937. The phage is nonhazardous to normal microbial flora, resistant to high temperature and high in stability andhas good application and development prospect, DNA of the phage cannot code virulence genes, and an excellent strain resource is provided for developing novel antibacterial preparations.

The invention discloses a detection and quality control kit for hepatitis A viruses and noroviruses in a water sample, as well as a detecting method. The kit comprises a tube of HAV 2*RT-PCR mix, a tube of NV GI+GII type 2*RT-PCR mix, a tube of MS2 2*RT-PCR mix, a tube of RNase-free water, a tube of HAV positive control, a tube of NV GI+GII positive control and a tube of coliphage MS2(2.5*1010 pfu / mL). The detecting method comprises the following steps: 1, virus concentration; 2, virus RNA extraction; 3, coliphage MS2 standard curve establishment; 4, hepatitis A viruses, norovirus GI type, norovirus GII type and coliphage MS2 detection; 5, virus RNA recovery ratio determination; 6, result judgment. According to the detection and quality control kit for the hepatitis A viruses and noroviruses in the water sample, as well as the detecting method, the quality of the whole virus detection process is effectively controlled through the key steps of sample pretreatment, detection, result judgment and the like, the stability, accuracy and controllability of the detection process are ensured, and the detection technology blank in the field is filled.

Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.

The invention belongs to the technical field of biology, and particularly relates to a broad-spectrum escherichia coli phage and an application thereof. The escherichia coli phage WXLECP008 provided by the invention is a virulent phage, the phage has tolerance under the conditions that the pH is 4-10 and the temperature is minus 80 DEG C to 65 DEG C, and the titer of the phage is 3.5 * 10 <10> pfu / mL when the phage is cultured for 8 hours under the condition that the MOI is 0.1. The escherichia coli phage provided by the invention is a phage with broad spectrum and wide splitting decomposition spectrum, is high in titer and high in safety, can be massively propagated in non-pathogenic host bacteria, and can be used for large-scale fermentation production. The phage provided by the invention can provide an excellent strain resource for developing a bacteriophage therapy, has a good application and development prospect, and is suitable for popularization and application.

The invention discloses an Escherichia coli bacteriophage capable of reducing antibiotic resistance and application thereof. A host of the Escherichia coli bacteriophage is Escherichia coli, and observation through an electron microscope shows that the bacteriophage is polyhedral and stereosymmetric, wraps nucleic acid, has the diameter of about 61nm, has no tail, and has the preservation number of CGMCC No. 22493. The Escherichia coli phage RDP-EC-20059 has a strong cracking effect on Escherichia coli in a poultry breeding environment, has relatively high temperature tolerance and a relatively wide acid-base tolerance range, can be used for efficiently treating Escherichia coli diseases caused by pathogenic bacteria in poultry breeding, can be used as a disinfectant, and can be applied to the field of poultry breeding. A bacteriophage source is provided for industrial, large-scale and programmed production of bacteriophages and treatment of poultry breeding bacterial diseases caused by escherichia coli.

The invention discloses separation and application of a lytic escherichia coli phage RDP-EC-16029. The host of the phage is avian escherichia coli E10, and the phage can form a plaque with a diameterof 0.5-1mm on double plates. Through observation by an electron microscope, the phage has a polyhedral stereosymmetric head which wraps nucleic acid and has a diameter of about 70nm; the phage has a tail with a length of about 120nm and has a tail sheath; a neck is connected with the head and the tail; and the phage belongs to myoviridae of caudovirales. The phage can maintain good activity in a pH range of 6.0-9.0, and has the highest phage titer when the pH value is 8.0, and the phage titer is basically unchanged in a range of 30-40 DEG C. The phage can efficiently infect the host strain avian escherichia coli E10, has a strong lysis effect on the avian escherichia coli E10 in a culture environment, and provides a phage source for industrial production of phages for prevention and treatment of the avian escherichia coli E10.

The invention belongs to the technical field of biology, and particularly relates to a combined bacteriophage preparation for treating piglet diarrhea. The bacteriophage comprises an escherichia colibacteriophage vB _ EcoP _ E21, an escherichia coli bacteriophage vB _ EcoM _ F2 and a shigella bacteriophage vB _ SsoM _ Z31, the preservation number of the escherichia coli bacteriophage vB_EcoP_E21is shown as follows: vB_EcoP_E21; the preservation number of the escherichia coli bacteriophage vB _ EcoM _ F2 is CGMCC 18871, and the preservation number of the shigella bacteriophage vB _ SsoM _ Z31is CGMCC 18870. The combined bacteriophage preparation is applied in a direct drenching mode, the abundance of escherichia coli and shigella can be reduced, diarrhea diseases of piglets in the breeding process are effectively prevented and treated, the survival rate of the piglets is increased, and economic losses are reduced. The combined bacteriophage preparation provided by the invention can replace traditional feed antibiotics, has the characteristics of safety, high efficiency, no residue and the like, and is an ideal antibiotic substitute.

The invention relates to preparation, an application and a kit of a coliphage M13 internal standard quality-control product. The coliphage M13 internal standard quality-control product prepared by the method serving as an internal standard quality-control substance in a fluorescence PCR nucleic acid kit of DNA pathogen can be used for accurately monitoring the whole experiment process in PCR detection of the DNA pathogen, which can be used as the standard for determining whether the experiment is successful or not. The coliphage M13 internal standard quality-control product has good stability, is easy to store (the freeze-dried storage time is more than or equal to 1 year), is convenient to transport and high in safety, and can be used for truly realizing whole-course quality detection from sample treatment to amplification by serving as the internal standard quality control product.

The invention relates to preparation of rumen bypass coated bacteriophage and an application of the rumen bypass coated bacteriophage in prevention and treatment of buffalo diarrhea. The rumen bypass coated bacteriophage is prepared by compounding six bacteriophages, comprising a staphylococcus aureus bacteriophage 52, a staphylococcus aureus subspecies bacteriophage B1, an escherichia coli bacteriophage T1, an escherichia coli bacteriophage T3, an escherichia coli bacteriophage S13 and a bacillus subtilis bacteriophage phi-e. The rumen bypass coated bacteriophage provided by the invention is added into a buffalo concentrate supplement according to the proportion that 30-70kg of the rumen bypass coated bacteriophage is added into each ton of concentrate. The rumen bypass coated bacteriophage is administrated to buffalo in a rumen bypass coating mode, the effect of preventing and treating buffalo diarrhea is ideal, and reference is provided for research of screening specific bacteriophages in the later period.

The invention discloses a phage-containing spray type environment disinfectant as well as a preparation method and an application thereof, which belongs to the technical field of biological disinfection, the effective components of the disinfectant comprise an escherichia coli bacteriophage CCTCC NO.M2016539, a staphylococcus aureus bacteriophage CCTCC NO.M2016535 and a hemolytic streptococcus bacteriophage CCTCC NO.M2019227, wherein the ratio of the effective components is 1: 1: 1; when the disinfectant with the titer larger than or equal to 1*10<10>pfu / ml is sprayed in the living environmentaccording to the dosage of 50-100 ml / m<3>, the disinfectant has a broad-spectrum bactericidal effect on common pathogenic bacteria and is high in specificity, a SM buffer solution and cane sugar canprotect the stability of the titer of bacteriophage, and long-term use and storage of the disinfectant are facilitated; the purifying effect on living environment is good, safe and non-toxic.

The invention relates to a preparation method and application of a coliphage MS2 internal standard quality control product and a kit. The coliphage MS2 internal standard quality control product prepared by the method, as an internal standard quality control material of a kit for RNA pathogen fluorescent RT-PCR nucleic acid detection, can be used for accurately monitoring an entire experiment process in the RNA pathogen RT-PCR detection, so that a standard for judging whether the experiment is successful or not is provided. The coliphage MS2 internal standard quality control product prepared by the method is good in stability, easy to be preserved (more than or equal to 1 year under a state of being freeze-dried), convenient to transport and high in safety; and the coliphage MS2 internal standard quality control product, as an internal standard quality control product, can really achieve whole-process quality detection from sample processing to amplification.

The invention discloses a test and quality control kit of Norovirus in shellfish and a non-diagnostic test method. The kit comprises one tube of NVGI+GII parting 2*RT-PCR mix, MS2 2*RT-PCR mix, RNase free water, NV GI+GII positive control and coliphage MS2 (2.5*1010 pfu / mL) respectively. The non-diagnostic test method comprises the following steps: (1) enrichment of Norovirus in shellfish; (2) extraction of virus RNA; (3) establishment of a coliphage MS2 standard curve; (4) test of the Norovirus and the coliphage MS2; (5) determination of the recovery rate of the virus RNA; (6) determination of results. According to the test and quality control kit of the Norovirus in shellfish and the non-diagnostic test method, the quality is effectively guaranteed in the whole virus detection process of critical steps of sample pretreatment, virus concentration, quality control, test, result determination and the like, stability, accuracy and controllability of the test process are enhanced, and the detection technology blank in the field is filled.

The invention provides a preparation method of a pseudovirus, an existing vector is modified, the modified vector comprises optimized sequences of capsid protein genes of escherichia coli MS2 bacteriophage, wherein the sequences are one or more of SEQ ID NO.1-6, and the modified vector is used for transforming bacteria and expressing to obtain the pseudovirus with higher packaging efficiency. The long-term storage stability of the pseudovirus is enhanced, and the pseudovirus can be freeze-dried and can be stored at room temperature for a longer time.

The invention discloses a spray disinfectant containing a phage composition in a breeding environment and a preparation method and application thereof. The spray disinfectant comprises phage or its composition with a total concentration greater than 1010PFU / mL and a streptococcus suis lyase LY7917 with a concentration of 100.0 [mu]g / mL according to a volume ratio of 0.1-100: 1. The phage is derived from a breeding environment and is selected from one or more of escherichia coli phage Ecp2, escherichia coli phage E20-1 and staphylococcus aureus phage. According to the spray disinfectant, a spray sterilization mode is adopted, the synergistic sterilization effect of phage and lyase can be effectively achieved, the total number of bacteria on the ground of an animal house, in air and in a feeding trough is remarkably reduced, the overall disinfection efficiency is superior to that of a chemical disinfectant glutaraldehyde decyl methylammonium bromide solution, and the spray disinfectant is an environment-friendly disinfectant for the livestock and poultry breeding environment.

The invention discloses a bacteriophage composition and an application of the bacteriophage composition in a medicine for preventing and treating chicken salpingitis. The bacteriophage composition comprises a coliphage PD07 and a salmonella bacteriophage PYC04. A preservation number of the coliphage PD07 is CGMCC No18865, and the coliphage PD07 is preserved in CGMCC on November 4, 2019; the preservation number of the salmonella bacteriophage PYC04 is CGMCC No18867, and the salmonella bacteriophage PYC04 is preserved in the CGMCC on November 4, 2019. According to the bacteriophage composition,the coliphage PD07 and the salmonella bacteriophage PYC04 are compounded to be used as a component for effectively preventing and treating the chicken salpingitis; and compared with a single lytic escherichia coli phage, the bacteriophage composition has advantages that a sterilization range is greatly widened; sterilization activity is improved; a laying rate of laying hens can be increased, andphysique and disease resistance of the laying hens are enhanced.

The invention provides a coliphage and application thereof, as well as a bactericidal composition and application of the bactericidal composition. The coliphage FM1017 is preserved in the China General Microbiological Culture Collection Center on December 27, 2019, has a preservation number of CGMCC No.19156, and is classified and named coliphage. The coliphage can be used for cracking enteropathogenic escherichia coli in a living body and cracking enteropathogenic escherichia coli in the environment independently or by combining with other substances, so that a safe and nontoxic phage disinfecting and sterilizing product is provided to environment disinfection and purification. The coliphage can be used for developing medicines for preventing or treating infection of enteropathogenic escherichia coli, and a using method for preventing or treating infection of enteropathogenic escherichia coli with the coliphage is established.

The invention discloses a phage composition formed by compounding coliphage PD06 and coliphage PD114 based on a cocktail prevention and control method, and its application in medicines for preventing and treating colibacillosis in chickens. The above-mentioned phage composition is a biological antibacterial agent, disinfectant and food additive with active ingredients, which can not only quickly, efficiently and safely kill pathogenic E. The line cleaner can effectively degrade the biofilm formed by the enrichment of Escherichia coli in the water line, and has the characteristics of purifying drinking water. The phage composition also has the advantages of safety, no residue, and no side effects.

The invention specifically provides a chicken Escherichia coli biological disinfectant. The chicken Escherichia coli bacteriophages Ecp2, Ecp3 and Ecp6 are adopted as raw materials, the chicken Escherichia coli biological disinfectant has a good disinfection effect on chicken colibacillosis outbreak henhouses, the disinfection effect of the chicken Escherichia coli biological disinfectant provided by the invention is better than that of didecyl ammonium bromide, the chicken Escherichia coli biological disinfectant is short in action time and fast in action, the total number of bacteria in a chicken coop can be obviously reduced, the bacteria amount is reduced to 0.30% of that of a control group after spraying disinfection is carried out for 30 minutes, the bacteria amount is only 0.10% of that of the control group after spraying disinfection is carried out for 70 minutes, and the killing effect on the total number of bacteria is more obvious than that of a didecyl ammonium bromide disinfectant. The disinfectant has good effects on developing green and pollution-free environment disinfectants and improving the economic benefits of broiler chicken breeding.

The invention discloses a bacteriophage composition which is formed by compounding an escherichia coli bacteriophage PD06 and an escherichia coli bacteriophage PD114 and is constructed on the basis ofa cocktail prevention and control method, and an application of the bacteriophage composition in a medicament for preventing and treating chicken colibacillosis. The invention also discloses a biological bacteriostatic agent, disinfectant and food additive which take the bacteriophage composition as an active component. The bacteriophage composition not only can quickly, efficiently and safely kill pathogenic escherichia coli and even drug-resistant escherichia coli, but also can effectively reduce the death rate of sick chicks. As a waterline cleaning agent, the bacteriophage composition caneffectively degrade biofilms formed by enrichment of escherichia coli in a waterline, and can purify drinking water. In addition, the bacteriophage composition also has the advantages of safety, no residue and no side effect.

The present invention discloses an coliphage vB_EcoM_swi3 with a preservation number of CCTCC NO: M 2019467. The phage belongs to the family Myocaudidae and has a polyhedral head and a retractable tail structure. The diameter of the head is about 80 nm, and the length of the tail is about 120 nm. ; On the solid medium, translucent plaques can be formed without halos around, with clear and regular edges and a diameter of about 1-1.5mm; the phage is effective against E. Chicken-derived pathogenic Escherichia coli has a good lysis effect, and has very broad application prospects in many aspects such as pharmaceutical preparations and feed additives for the prevention and treatment of porcine colibacillosis and chicken colibacillosis.