Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A short-tailed coliphage and its application

A technology of Escherichia coli and phage, which is applied in the direction of phage, virus/phage, application, etc., can solve the problems of difficult prevention and treatment of Escherichia coli disease, resistance to vigorous development of aquaculture industry, serious bacterial resistance, etc., achieves no toxic side effects, and reduces chicken Mortality, effects of strong lytic activity

Active Publication Date: 2021-03-02
QINGDAO PHAGEPHARM BIO TECH CO LTD
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In my country, due to the popularization and abuse of antibiotics, drug resistance of bacteria in our country is very common, and cross multiple drug resistances are common in Escherichia coli, making it more and more difficult to prevent and control colibacillosis
The large-dose, large-area and high-frequency use of antibiotics has increased the cost of breeding, and the resistance of bacteria has become more and more serious, which has caused great resistance to the vigorous development of the breeding industry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A short-tailed coliphage and its application
  • A short-tailed coliphage and its application
  • A short-tailed coliphage and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and purification of phage PD38

[0036] The manure sewage sample among the present invention is collected from a chicken farm in Heze, Shandong;

[0037] The host bacterium is pathogenic Escherichia coli from chicken.

[0038] Pick the host bacteria liquid, line three zones on the SS agar medium, and cultivate in a 37°C incubator for 16-24 hours to obtain a single colony. A single colony was picked, inoculated into a 5 mL LB broth test tube, and cultured overnight at 37° C. with shaking at 170 rpm to obtain a host bacterial liquid. Take 50mL of manure sewage from a chicken farm in Heze, Shandong, add 500μL of host bacteria suspension, then add LB broth to 200mL, soak and incubate overnight at 37°C. The next day, 5 mL of the liquid was taken out, centrifuged at 10,000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm sterile microporous membrane to obtain a phage stock solution, which was stored at 4°C.

[0039] Use the double-laye...

Embodiment 2

[0041] The biological characteristic of embodiment 2 bacteriophage

[0042] 1. Morphological characteristics of phage PD38

[0043] Phage PD38 was observed under a transmission electron microscope (see figure 2 ). The phage has a polyhedral head structure with a diameter of about 75 nm and six short tail filaments. According to the classification of phages, the morphology of the phages in this study conforms to the characteristics of the Brachyphage family and belongs to Brachyphages.

[0044] 2. Effect of temperature on phage PD38

[0045] Take 100 μL phage PD38 proliferation solution (titer is 2.00×10 10 PFU / mL) were dispensed into sterile EP tubes, and reacted in water baths at 40°C, 50°C, 60°C, 70°C, and 80°C for 20min, 40min, and 60min, respectively. Two replicates were set for each temperature. After the reaction was over, samples were taken, and the samples were immediately cooled in an ice bath, and the titer of the phage was determined by the double-layer plate m...

Embodiment 3

[0057] The safety test of embodiment 3 bacteriophage

[0058] Select 20 1-day-old SPF chicks and randomly divide them into two groups: the test group and the blank control group, and the test group is given 2×10 10 PFU / mL / 0.25mL / bird, the test group was fed with an equal volume of sterile normal saline for 7 days in a row, and the behavior and growth of the chickens were observed. Tract and mucosal changes.

[0059] It was found that the chickens in the test group and the control group had the same growth conditions, good mental state, normal food intake and drinking water, and no adverse reactions. There were no abnormalities in the internal organs and digestive tract mucosa of the chickens in the two groups, thus confirming that the bacteriophage PD38 is safe and has no toxic side effects. .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a coli phage and its application, in particular to a short-tailed strong lytic coli phage. The coliphage mentioned above was named PD38, and it was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation date was September 26, 2018, and the preservation number was CGMCC No.16391. The bacteriophage has a good cracking effect on E. coli, has a good disinfecting effect on E. coli in food and breeding environments, and can prevent diseases caused by chicken E. coli.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strong lytic bacteriophage of short-tailed Escherichia coli and its application in food sterilization, space environment and poultry Escherichia coli diarrheal disease. Background technique [0002] Escherichia coli is an opportunistic pathogen, and the pathogenicity is related to the serotype of Escherichia coli, and there are differences among different animals. Once chickens are infected with Escherichia coli, the mortality rate will rise sharply, causing diarrhea, which will obviously affect the weight gain of chickens, and will also affect the egg production rate of laying hens. The main source of infection of colibacillosis in chickens is sick chickens and healthy chickens carrying E. coli. The chickens themselves can also infect other healthy chickens through feces and secreted mucus, causing large-scale infection. The E. coli in the breeding environment remains ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04A23K10/18A23L3/3571A01N63/40A01P1/00C12R1/92
CPCA01N63/00A23L3/3571A61K35/76A23K10/18A61P31/04C12N7/00C12N2795/10221C12N2795/10231C12N2795/10232
Inventor 潘强任慧英孙虎芝刘广芹
Owner QINGDAO PHAGEPHARM BIO TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com