Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Escherichia coli bacteriophage capable of reducing antibiotic resistance and application thereof

An antibiotic resistance, Escherichia coli technology, applied in the preservation of bacteriophages, viruses/phages, food ingredients as antimicrobials, etc. Application prospect, strong cleavage activity, high titer effect

Active Publication Date: 2021-11-02
RECOM QINGDAO BIOTECH CO LTD
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a strain of E. coli phage that can reduce antibiotic resistance, to replace the application of antibiotics in the prevention and treatment of poultry breeding, and to solve the problem of acute onset of poultry animals and untimely treatment. lead to problems such as drug ineffectiveness

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Escherichia coli bacteriophage capable of reducing antibiotic resistance and application thereof
  • Escherichia coli bacteriophage capable of reducing antibiotic resistance and application thereof
  • Escherichia coli bacteriophage capable of reducing antibiotic resistance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 Isolation and identification of pathogenic bacteria and bacteriophage

[0045] (1) Isolation and identification of pathogenic Escherichia coli BE-20058

[0046] Sampling was taken from a chicken farm in Jimo, Qingdao, Shandong Province, and the liver of the diseased chicken was taken aseptically, and a line was drawn on the selective medium (MacConkey agar medium). After culturing at 37°C for 18-24 hours, a round, For red colonies with flat, neat edges, smooth and wet surface, pick typical colonies and continue to streak and purify for 3 times, then pick a single colony and inoculate it in 5mL LB broth, culture at 37°C and 200rpm for 8 hours to obtain a uniform turbid bacterial suspension liquid. It was identified as Escherichia coli through 16sRNA molecular identification, and its serotype was determined to be O78 through serum identification. O78 has strong pathogenicity and can be transmitted to humans through the food chain and other methods, with stro...

Embodiment 2

[0054] Electron microscope observation of embodiment 2 phage

[0055] Take 20 μL of the liquid containing crude phage particles and drop them on the copper grid, let it settle naturally for 15 minutes, and absorb the excess liquid from the side with filter paper, add a drop of 2% phosphotungstic acid (PTA) on the copper grid to stain the phage for 10 minutes, and then use the filter paper to remove the The staining solution was sucked off from the side, and the morphology of the phage was observed with an electron microscope after the sample was dried.

[0056] photo by electron microscope figure 1 It can be seen that the bacteriophage RDP-EC-20059 has a polyhedral stereosymmetric head, which wraps nucleic acid, has a diameter of about 61 nm, and has no tail.

Embodiment 3

[0057] Example 3 Phage Genome Sequencing

[0058] After the enrichment culture of a single phage, centrifuge at 8000g for 15 minutes at 4°C, add 10% PEG8000 and 0.5M NaCl and let it stand overnight, then add an equal amount of chloroform to mix well, and centrifuge at 5000g for 10 minutes after standing for stratification , after removing the chloroform layer and PEG layer, add restriction endonuclease for digestion treatment, and use gradient density cesium chloride to suspend phage, and then use TM buffer to dialyze 3 times, each time for 30 minutes, and send it to Huada Gene for biological sequencing The company performs whole genome sequencing. The results are shown in the sequence listing.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Incubation periodaaaaaaaaaa
Login to View More

Abstract

The invention discloses an Escherichia coli bacteriophage capable of reducing antibiotic resistance and application thereof. A host of the Escherichia coli bacteriophage is Escherichia coli, and observation through an electron microscope shows that the bacteriophage is polyhedral and stereosymmetric, wraps nucleic acid, has the diameter of about 61nm, has no tail, and has the preservation number of CGMCC No. 22493. The Escherichia coli phage RDP-EC-20059 has a strong cracking effect on Escherichia coli in a poultry breeding environment, has relatively high temperature tolerance and a relatively wide acid-base tolerance range, can be used for efficiently treating Escherichia coli diseases caused by pathogenic bacteria in poultry breeding, can be used as a disinfectant, and can be applied to the field of poultry breeding. A bacteriophage source is provided for industrial, large-scale and programmed production of bacteriophages and treatment of poultry breeding bacterial diseases caused by escherichia coli.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a coliphage strain capable of reducing antibiotic resistance and its application in coliform infection in poultry breeding environments. Background technique [0002] Avian colibacillosis is caused by certain pathogenic or conditionally pathogenic Escherichia coli. It is one of the most serious bacterial diseases that endanger the poultry industry. It is most likely to produce drug resistance during disease prevention and treatment, leading to antibiotics A series of problems such as increased consumption and drug residues in poultry products. The disease can occur throughout the year, but it occurs frequently in winter and spring and in periods of variable temperature, which brings serious economic losses to the poultry industry. Avian colibacillosis is mainly controlled by antibiotics clinically, but due to the blind addition or excessive use of antibiotics, the drug-resistant str...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A23K10/18A23K50/75A23B4/22A61K35/76A61P31/04A01N63/40A01P1/00C11D3/48C12R1/92
CPCC12N7/00A23K10/18A23K50/75A23B4/22A61K35/76A61P31/04A01N63/40C11D3/48C12N2795/00021C12N2795/00031A23V2002/00A23V2200/10
Inventor 杜新永李先胜陈平王立坤崔翠
Owner RECOM QINGDAO BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com