Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Coliphage and application thereof, as well as bactericidal composition and application of bactericidal composition

A technology of Escherichia coli and phage, which is applied in the direction of phage, virus/phage, medical raw materials derived from virus/phage, etc., and can solve problems such as body damage and high drug resistance of pathogenic bacteria

Inactive Publication Date: 2020-05-22
沈阳丰美生物技术有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of antibiotics will lead to extremely high drug resistance of pathogenic bacteria and cause great harm to the body, so effective alternatives are urgently needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Coliphage and application thereof, as well as bactericidal composition and application of bactericidal composition
  • Coliphage and application thereof, as well as bactericidal composition and application of bactericidal composition
  • Coliphage and application thereof, as well as bactericidal composition and application of bactericidal composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Phage isolation and purification

[0052] 1. Phage isolation

[0053] The sewage sample was centrifuged in a centrifuge at 4°C to discard impurities, and then filtered through a 0.22 μm microporous membrane to remove bacteria, and the supernatant was retained. Take the supernatant and add it to the LB liquid culture medium together with the host bacteria, at a temperature of 37°C and a rotation speed of 200r / min, and take it out after cultivating for 16-18 hours. Collect the mixed culture solution into a centrifuge tube, put it into a centrifuge at 4°C to remove bacteria, set the speed at 10000r / min, and centrifuge for 10min. The centrifuged phage supernatant was filtered through a 0.22 μm microporous membrane, and the obtained filtrate was the phage stock solution.

[0054] 2. Phage verification

[0055] Pipette 0.1 mL of phage stock solution and 0.1 mL of host bacteria cultured overnight and drop on the empty sterile culture. Pour the LB semi-agar medium at about ...

Embodiment 2

[0074] Determination of Optimal Growth Temperature of Phage

[0075] Adjust the temperature of the water bath in advance, put the phage in the water bath at 10°C, 20°C, 30°C, 37°C, and 40°C for 60 minutes, then take 100 μL of the phage liquid and 100 μL of the host bacteria liquid and use the double-layer agar plate method For the plaque test, after the plate solidified, it was cultured at the corresponding temperature, the experiment was repeated three times, and the culture was cultured for 10 hours or overnight, and the results were observed. Repeat the experiment three times.

[0076] Table 1 Phage plaque morphology at different temperatures

[0077]

[0078] The results of the optimum growth temperature of phage are shown in Table 1 and figure 1 , the results showed that: when the phage was at 37°C, the number of plaques was the highest, and the diameter of the plaques was the largest, that is, the activity was the strongest; below 20°C, the activity decreased rapidl...

Embodiment 3

[0080] pH Stability Experiment of Phage

[0081] Adjust the pH values ​​of the LB liquid medium to 3, 4, 5, 6, 7, 8, 9, 10, and 11 respectively, take 100 μL for each pH value, and add 100 μL of 10 8 The pfu / mL phage solution was mixed evenly and reacted at 37°C for 60min. Take 100 μL from each tube, lay a double-layer agar plate, incubate overnight at 37°C, count the plaques, and determine the titer of the phage. Repeat the experiment three times.

[0082] Table 2 Determination results of phage pH value stability (unit: pfu / mL)

[0083]

[0084]

[0085] The assay result of phage pH value stability is as table 2 and figure 2 As shown, the high activity is maintained at pH 4-12, indicating that the phage has a wide adaptability to the pH value; when the pH value is less than 4 or greater than 12, the activity of the phage decreases rapidly; 8 is the most stable, the highest survival rate, the strongest activity, and its optimum pH value should be 8.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Titeraaaaaaaaaa
Login to View More

Abstract

The invention provides a coliphage and application thereof, as well as a bactericidal composition and application of the bactericidal composition. The coliphage FM1017 is preserved in the China General Microbiological Culture Collection Center on December 27, 2019, has a preservation number of CGMCC No.19156, and is classified and named coliphage. The coliphage can be used for cracking enteropathogenic escherichia coli in a living body and cracking enteropathogenic escherichia coli in the environment independently or by combining with other substances, so that a safe and nontoxic phage disinfecting and sterilizing product is provided to environment disinfection and purification. The coliphage can be used for developing medicines for preventing or treating infection of enteropathogenic escherichia coli, and a using method for preventing or treating infection of enteropathogenic escherichia coli with the coliphage is established.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a coli phage and its application, a bactericidal composition and its application. Background technique [0002] In livestock farms, due to the existence of a large number of pathogenic bacteria, the environment and water sources are polluted by a large number of pathogenic bacteria. When the concentration of pathogenic bacteria reaches a certain value. It will cause pathogenic damage to the body of livestock and poultry. Among them, enteropathogenic Escherichia coli (Enterotoxigenic Escherichia coli, ETEC) widely exists in soil, water, agricultural products and forest products in nature, and is a type of diarrhea that causes human and young animals (newborn piglets, calves, lambs and weaned piglets). It is an important pathogenic bacterium. After the newborn young animals are infected by ETEC, they often die due to severe watery diarrhea and rapid dehydration, and the morbidity and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCC12N7/00A61K35/76A61P31/04C12N2795/00021Y02A50/30
Inventor 丁毅唐日益王丽娟曹岩峰解桂香黄竹姜丹
Owner 沈阳丰美生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com