Bacteriophage-containing spray type environment disinfectant as well as preparation method and application thereof

A technology of bacteriophage and disinfectant, which is applied in the field of spray-type environmental disinfectant and its preparation, can solve the problems of low phage titer, narrow application range, and short storage time, and achieve the goal of overcoming corrosive and irritating odors and wide application range , strong specific effect

Active Publication Date: 2020-06-23
中科纳迪(苏州)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is technical problems such as low titer of phage in the phage disinfectant in the prior art, narrow scope of application, and short storage time caused by poor stability

Method used

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  • Bacteriophage-containing spray type environment disinfectant as well as preparation method and application thereof
  • Bacteriophage-containing spray type environment disinfectant as well as preparation method and application thereof
  • Bacteriophage-containing spray type environment disinfectant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of bacteriophage disinfectant

[0033] After the purchased phages were preserved and purified in our laboratory, they were activated, dissolved in PBS and diluted to make the concentration of each phage ≥10 10 pfu / ml, and the concentrations of the three bacteriophages are equal, and then the other auxiliary materials are prepared to the corresponding concentration with ultrapure water, and then mixed with various auxiliary materials according to the following volume ratio, and mixed evenly, to obtain a disinfectant. The formula composition is as follows:

[0034] Coliphage CCTCC NO.M2016539 20%;

[0035] Staphylococcus aureus phage CCTCC NO.M2016535 20%;

[0036] Hemolytic streptococcal phage CCTCC NO.M2019227 20%;

[0037] 0.003M sodium dodecylsulfonate (SDS) solution 20%;

[0038] SM buffer 10%;

[0039] 0.1M Sucrose 10%.

[0040] Wherein, the preparation method of SM buffer comprises the following steps: adding 0.1g gelatin, 2g MgSO 4 ·7H 2 O and 5...

Embodiment 2

[0042] After the purchased phages were preserved and purified in our laboratory, they were activated, dissolved in PBS and diluted to make the concentration of each phage ≥10 10 pfu / ml, and the concentrations of the three bacteriophages are equal, and then the other auxiliary materials are prepared to the corresponding concentration with ultrapure water, and then mixed with various auxiliary materials according to the following volume ratio, and mixed evenly, to obtain a disinfectant. The formula composition is as follows:

[0043] Coliphage CCTCC NO.M2016539 10%;

[0044] Staphylococcus aureus phage CCTCC NO.M2016535 30%;

[0045] Hemolytic streptococcal phage CCTCC NO.M2019227 20%;

[0046] 0.003M sodium dodecylsulfonate (SDS) solution 20%;

[0047] SM buffer 10%;

[0048] 0.1M Sucrose 10%.

[0049] Wherein, the preparation method of SM buffer comprises the following steps: adding 0.1g gelatin, 2g MgSO 4 ·7H 2 O and 5.8 g NaCl.

Embodiment 3

[0051] After the purchased phages were preserved and purified in our laboratory, they were activated, dissolved in PBS and diluted to make the concentration of each phage equal to 10 9 pfu / ml, and then prepare other auxiliary materials to the corresponding concentration with ultrapure water, add various auxiliary materials according to the following volume ratio and mix them, and mix evenly to obtain a disinfectant. The formula composition is as follows:

[0052] Coliphage CCTCC NO.M2016539 20%;

[0053] Staphylococcus aureus phage CCTCC NO.M2016535 20%;

[0054] Hemolytic streptococcal phage CCTCC NO.M2019227 20%;

[0055] 0.003M sodium dodecylsulfonate (SDS) solution 20%;

[0056] SM buffer 10%;

[0057] 0.1M Sucrose 10%.

[0058] Wherein, the preparation method of SM buffer comprises the following steps: adding 0.1g gelatin, 2g MgSO 4 ·7H 2 O and 5.8 g NaCl.

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Abstract

The invention discloses a phage-containing spray type environment disinfectant as well as a preparation method and an application thereof, which belongs to the technical field of biological disinfection, the effective components of the disinfectant comprise an escherichia coli bacteriophage CCTCC NO.M2016539, a staphylococcus aureus bacteriophage CCTCC NO.M2016535 and a hemolytic streptococcus bacteriophage CCTCC NO.M2019227, wherein the ratio of the effective components is 1: 1: 1; when the disinfectant with the titer larger than or equal to 1*10<10>pfu / ml is sprayed in the living environmentaccording to the dosage of 50-100 ml / m<3>, the disinfectant has a broad-spectrum bactericidal effect on common pathogenic bacteria and is high in specificity, a SM buffer solution and cane sugar canprotect the stability of the titer of bacteriophage, and long-term use and storage of the disinfectant are facilitated; the purifying effect on living environment is good, safe and non-toxic.

Description

technical field [0001] The invention belongs to the technical field of biological disinfection, and in particular relates to a spray-type environmental disinfectant containing bacteriophage and its preparation method and application. Background technique [0002] Escherichia coli is a Gram-negative facultative anaerobic rod-shaped bacterium belonging to the genus Escherichia in the family Enterobacteriaceae. They mainly live in the intestinal tract of humans and animals, and together with other facultative anaerobic bacteria occupy 0.9% of the intestinal flora. The vast majority of E. coli are harmless to the human body, and even help digest food and maintain human immunity force. However, studies have found that a small number of serotypes of Escherichia coli have strong pathogenicity to the body, and they can invade the human gastrointestinal tract to cause disease. The reason is that there are potential virulence factors, such as Shiga-like toxin, specific adhesin and E...

Claims

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Application Information

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IPC IPC(8): A01N63/40A01N25/06A01P1/00C12N7/00
CPCA01N63/00A01N25/06C12N7/00
Inventor 苏鑫崔新
Owner 中科纳迪(苏州)科技有限公司
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