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Boletus speciosus Frost polysaccharide BSF-X as well as preparation method and application thereof

A BSF-X, bolete technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., to achieve the effect of improving the ability to phagocytose neutral red, improve the secretion ability, and improve the immunoregulatory activity

Active Publication Date: 2019-01-18
西充星河生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there has not been any report on the fine structure, immune activity, anti-tumor research and application of the boletus polysaccharide BSF-X

Method used

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  • Boletus speciosus Frost polysaccharide BSF-X as well as preparation method and application thereof
  • Boletus speciosus Frost polysaccharide BSF-X as well as preparation method and application thereof
  • Boletus speciosus Frost polysaccharide BSF-X as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0043] Bolete boletus fruiting body powder after crushing and distilled water are boiled in a water bath at 100°C for 3 hours at a volume ratio of 1:2, boiled 3 times, and the supernatant is collected and concentrated; add 5 times the volume of the prepared concentrated solution Absolute ethanol forms a flocculent precipitate, collects the precipitate and dries to obtain the crude extract of the polysaccharide of Boletus chinensis; the crude extract is separated and purified by anion exchange column chromatography, and 50.00g of cellulose is accurately weighed with an electronic balance. The column was prepared with 0.12 mol / L NaCl solution for elution, the eluate was collected and its purity was tested by HPGPC, and the polysaccharide BSF-X of Boletus chinensis was prepared.

Embodiment 3

[0045] Bolete boletus fruiting body powder after crushing and distilled water are boiled in a water bath at 100°C for 6 hours at a volume ratio of 1:5, boiled 3 times, and the supernatant is collected and concentrated; add 3 times the volume to the prepared concentrated solution Absolute ethanol forms a flocculent precipitate, collects the precipitate and dries to obtain the crude extract of the polysaccharide of Boletus chinensis; the crude extract is separated and purified by anion exchange column chromatography, and 50.00g of cellulose is accurately weighed with an electronic balance. The column was prepared with 0.12 mol / L NaCl solution for elution, the eluate was collected and its purity was tested by HPGPC, and the polysaccharide BSF-X of Boletus chinensis was prepared.

[0046] Embodiment 4 Structural identification of boletus huamei polysaccharide BSF-X

[0047] Using chemical methods (acid hydrolysis, methylation analysis) and spectroscopic techniques (IR, HPLC, GC-MS...

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Abstract

The invention discloses boletus speciosus Frost polysaccharide BSF-X as well as a preparation method and an application thereof. The boletus speciosus Frost polysaccharide mainly comprises beta-D-glucose and alpha-D-galactose in the ratio being 2:1, has the average molecular weight of about 141309 Da, has a main chain of (1 arrow 4)-beta-D-glucose, a side chain of arrow 1,6)-alpha-D-galactose connected on 6-O and a arrow 4)-beta-D-glucose connected on galactose 2-O of the side chain. The boletus speciosus Frost polysaccharide has higher immunoregulation activity and can significantly or very significantly promote proliferation of three immune cells including B, T and Raw264.7; BSF-X can promote proliferation of cells, promote macrophage to release immune factors IL-23 and TNF-alpha, has aquite significant inhibition function on L929 in vitro and can inhibit growth of S180 tumor in vivo.

Description

technical field [0001] The invention belongs to the technical field of research and development of fungal polysaccharides, and in particular relates to a polysaccharide BSF-X from Boletus chinensis and its preparation method and application. Background technique [0002] Polysaccharides, or polysaccharides, are a class of biomacromolecules ubiquitous in living organisms, often related to the composition of the cytoskeleton and the composition of various endogenous bioactive molecules. Modern biochemistry has always regarded polysaccharides as substances that maintain cell structures (such as cellulose and chitin) and provide energy sources (such as starch and glycogen) in organisms, but have not paid enough attention. The development of polysaccharides is much later than that of proteins and nucleic acids. In addition, the complexity and diversity of polysaccharides pose great challenges to the determination and identification of polysaccharide structures. Currently, resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P35/00
CPCA61K31/715C08B37/0003C08B37/006
Inventor 侯怡铃丁祥宋波
Owner 西充星河生物科技有限公司
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