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Kluyveromyces marxianus and application thereof

A technology of yeast and yeast extract, applied in the field of yeast, can solve problems affecting human health and achieve high protein secretion ability and high biomass effect

Active Publication Date: 2015-11-25
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] Industrial enzyme preparations are derived from organisms, but because they are usually not pure enzymes, relevant components in the products (such as certain metabolites of microorganisms, or even harmful substances) may be carried along with the food during use. into, thereby affecting human health

Method used

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  • Kluyveromyces marxianus and application thereof
  • Kluyveromyces marxianus and application thereof
  • Kluyveromyces marxianus and application thereof

Examples

Experimental program
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Embodiment Construction

[0030] Sample Collection and Yeast Isolation:

[0031] Different yoghurt samples were collected from the Nagchu region of Tibet in August 2014.

[0032] Take 1g of yogurt sample, dilute it with 50ml of sterile water, then dilute it 10 times, 100 times, 1000 times and 10000 times with sterile water, take 100μl of the diluted solution and spread it on the yeast solid medium YNB (amino-free yeast nitrogen source ; YeastNitrogenBasewithoutAminoAcids) + 1% inulin plate, 30 ° C after 3-4 days to form colonies.

[0033] filter

[0034] The yeast isolated from the yogurt sample and two Kluyveromyces strains Kluyveromyceslactis (preservation number: ATCC8585) and K. % glucose liquid medium, 30 DEG C for 120 h after fermentation, the supernatant was collected by centrifugation, and then protein electrophoresis technology-SDS polyacrylamide gel electrophoresis (SDS-PAGE) was used to detect the ability of each strain of yeast to secrete proteins.

[0035] Among them, it was found t...

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Abstract

The invention provides a kluyveromyces marxianus with a preservation number of CGMCC No. 10621, and also provides the application of the kluyveromyces marxianus. Compared with existing kluyveromyces marxious such as ATCC8585 and ATCC26548, the kluyveromyces marxianus has higher protein secretion capacity and higher biomass, can be used for producing enzymic preparations, and also can be used for building a recombinant protein expression system.

Description

technical field [0001] The invention relates to a yeast, in particular to a Kluyveromyces marx with high secretion performance and high biomass, and an application thereof. Background technique [0002] At present, there are more than 100 known enzyme preparations, and more than 30 are commonly used, which are widely used in food, fermentation, leather, textile, daily chemical, pharmaceutical and other industries. There are 15 kinds that can be used in the food industry in our country. [0003] Industrial enzyme preparations are derived from organisms, but because they are usually not pure enzymes, relevant components in the products (such as certain metabolites of microorganisms, or even harmful substances) may be carried along with the food during use. into, thereby affecting human health. The United States, Canada, the European Union, Australia, New Zealand, Japan and other countries all stipulate that enzyme preparations must undergo strict safety evaluation and can on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N9/24
Inventor 吕红周峻岗余垚袁汉英李育阳
Owner FUDAN UNIV
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