The invention provides a novel method for separating / enriching trace
roxithromycin in environment. The method comprises the following steps: adding 0.4mg.mL<-1> researched
roxithromycin test solution in a mouth polished colorimetric tube, wherein the volume of the
roxithromycin test solution accounts for 1 / 50 of total volume of total solution in sublation; adding
solid salt to adjust the
ionic strength of the solution, shaking the solution to ensure that the solution can be sufficiently dissolved, and adjusting pH to be between 10.8 and 11.8, wherein the salt is
solid salt of Na2CO3, (NH4)2SO4, NaH2PO4, MgSO4, Na2SO3 and NaOH; transferring the solution into a sublation column, adding the sublation
solvent ionic liquid [Bmim]BF4 into the sublation column, and bringing to total volume by adding water, wherein the volume of the
ionic liquid [Bmim]BF4 accounts for 1 / 25 of total volume of total solution in sublation, and the sublation flow rate of N2 is between 5 and 25mL.min<-1>; floating for 5 to 60 minutes, standing for 1 minute after
aeration is stopped; taking out upper enriched [Bmim]BF4 phase by a
burette when no
air bubble exists; adding a certain amount of 75 percent sulphuric acid and 12 to 19 mL / mg of ROX into the sublation column to perform a color development reaction at
room temperature for 0 to 70 minutes; analyzing and determining the content of the solution by using a molecule
fluorescence photometry in 350nm
excitation wavelength and 508nm emission
wavelength. The method optimizes the best condition of sublation, and carries out separation / enrichment analysis on the trace roxithromycin in an actual
environmental water sample by combining the molecule
fluorescence photometry under an optimized testing condition.