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Preparation method and application of sensor for simultaneously detecting four aminoglycoside antibiotics

A sensor, the technology of hydroxypropyl chitosan, applied in the field of new functional materials and biosensing analysis, can solve the problems of immune sensors that have not been seen yet, and achieve easy miniaturization and integration, portability and wide application range Effect

Active Publication Date: 2013-08-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, there have been no reports on the one-time simultaneous detection of streptomycin, gentamicin, kanamycin and spectinomycin four aminoglycoside antibiotics immunosensors made of screen-printed electrodes

Method used

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  • Preparation method and application of sensor for simultaneously detecting four aminoglycoside antibiotics

Examples

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Effect test

Embodiment 1

[0033] The preparation method of the sensor for simultaneous detection of four aminoglycoside antibiotics described in the present invention

[0034] combine figure 1 The steps are as follows: on the surface of a rectangular substrate ① coated with a layer of polyester film, one reference electrode ② is printed with silver paste, 4 working electrodes ③, one counter electrode ④ are printed with conductive carbon paste, 6 electrode lead-out wires ⑤ and the end of the lead-out wire ⑥, and a layer of insulating layer ⑦ is printed with insulating paste on the substrate other than the ends of all electrodes and lead-out wires, and a circular electrolytic cell groove is formed in the electrode area ⑧ , and a four-channel screen-printed electrode was prepared.

Embodiment 2

[0036] The preparation method of the simultaneous detection of four kinds of aminoglycoside antibiotic sensors according to the present invention combines figure 1 ,Proceed as follows.

[0037] (1) On the 4 working electrodes of the four-channel screen-printed electrode prepared in Example 1, drop-coat 5 μL of platinum nanoparticles-sulfonated graphene-carboxymethyl chitosan conductive composite nanomaterial; the conductive composite nano Material solution, the preparation steps are as follows: the platinum nanoparticles and sulfonated graphene were mixed and ultrasonically dispersed for 30 minutes, and then a carboxymethyl chitosan solution with a mass fraction of 1.0% was added and ultrasonically mixed for 30 minutes to make a mass ratio of 1: 6: 1 Platinum nanoparticles-sulfonated graphene-carboxymethyl chitosan conductive composite nanomaterial solution.

[0038] (2) Add 5 μL of streptomycin antibody, gentamicin antibody, kanamycin antibody and spectinomycin antibody at a...

Embodiment 3

[0040] Change the amount of drop-coated platinum nanoparticles-sulfonated graphene-carboxymethyl chitosan conductive composite nanomaterial solution to 10 μL, the mass ratio is 2 : 10 : 10, and the platinum nanoparticles and sulfonated graphene are ultrasonically mixed for 20 min. Add carboxymethyl chitosan solution and ultrasonically mix for 20 min, and the others are the same as in Example 2.

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Abstract

The invention discloses a preparation method and an application of a sensor for simultaneously detecting four aminoglycoside antibiotics and belongs to the technical field of novel function materials and biosensor analysis. According to the invention, a reference electrode is printed by the use of a silver paste and four working electrodes and a counter electrode are printed by the use of a conductive carbon paste on a rectangular substrate surface coated with a layer of a polyester film. Thus, a four-channel screen-printed electrode is prepared. The preparation method is characterized in that antibodies of four aminoglycoside antibiotics including streptomycin, gentamycin, kanamycins and spectinomycin are respectively fixed on surfaces of the four working electrodes by the use of a Pt nanoparticle-sulfated graphene-carboxymethyl chitosan conductive composite nano-material; and after the detection liquids are dripped, the four working electrodes are connected with a multichannel electrochemical workstation detection circuit so as to realize simultaneous detection of the four aminoglycoside antibiotics. The sensor provided by the invention is simple to prepare and convenient to process, requires low cost, and is convenient to carry. The detection method is simple and rapid and has high sensitivity and good specificity.

Description

technical field [0001] The invention relates to a preparation method and application of a sensor for simultaneously detecting four kinds of aminoglycoside antibiotics, and belongs to the technical field of new functional materials and biosensing analysis. Background technique [0002] Antibiotics are drugs against pathogenic microorganisms. They are a class of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher animals and plants during their life. Other chemical substances that function in the development of living cells. [0003] At present, the detection methods for antibiotic residues mainly include high-performance liquid chromatography (HPLC for short), liquid chromatography-tandem mass spectrometry (LC-MS / MS for short), microbial method (MA for short), and enzyme-linked immunosorbent assay ( ELISA for short), the characteristics of these methods are as follows: Although the HPLC method has high analytical efficien...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30G01N33/569
Inventor 魏琴于海琴张勇杜斌李贺吴丹马洪敏
Owner UNIV OF JINAN
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