75 results about "Higher animals" patented technology

The invention belongs to the field of pharmaceutical formulations specially used for animals, and relates to an in-situ gel formulation for florfenicol injection and a preparation method thereof. The in-situ gel formulation is prepared from antibacterial drugs, an in-situ gel substratum and other indispensable auxiliary materials, wherein the antibacterial drugs are specially used for animals; the in-situ gel substratum is accredited in the field. The preparation method is illustrated.The in-situ gel formulation disclosed by the invention is freely flowing liquid at room temperature, forms the semisolid gel after intramuscular injection or hypodermic injection administration, realizes only one-time administration within one treatment course, has the advantages of easiness for preparation, stability in property, convenience for administration, long administration effect lasting time, definite treatment effect, high animal adaptability, no toxic or side effect or adverse reaction, and the like, achieves the broad-spectrum antibacterial effect and is suitable for preventing and treating various sensitive bacteria infection of the animals, such as pigs, sheep, dogs, cats, cattle, and the like.

The invention relates to a later-period compound feed for environment-friendly nonresistant broilers. The compound feed has high animal digestion utilization rate, can maintain the flora balance of digestive tracts effectively and is low in cost, and environment pollution is reduced. The later-period compound feed is prepared from the following raw materials in part by weight: 580 to 630 parts ofcorn, 90 to 120 parts of bean pulp, 85 to 105 parts of wheat middlings, 25 to 45 parts of vegetable dregs, 50 to 70 parts of distiller dried grain with soluble (DDGS), 20 to 40 parts of corn albumen powder, 20 to 30 parts of meat bone meal, 3 to 7 parts of calcium carbonate, 0.3 to 0.7 part of composite vitamin, 10 to 30 parts of grease, 2.0 to 6.0 parts of lysine, 0.2 to 0.6 part of methionine, 0.1 to 0.5 part of threonine, 2.0 to 3.0 parts of table salt, 0.3 to 0.7 part of antioxidant, 0.3 to 0.7 part of microecological preparation and 0.1 to 0.5 part of phytase.

The invention discloses a method for preventing and curing tobacco mosaic virus disease which includes the following steps of extracting a RNA gene group of the tobacco mosaic virus; utilizing the RNA gene group of the tobacco mosaic virus to acquire a cDNA of movement protein of the tobacco mosaic virus by reverse transcription; utilizing a PCR technology to separate a section of a 21-23bp length in 150-800bp on the cDNA of the movement protein of the tobacco mosaic virus; cloning the section into a plasmid carrier that can express the RNA so as to lead the cloned section to be expressed as the RNA in a colon bacillus of an enzyme lacking the RNA; crushing a cell and making the cell into a suspension solution with the chroma of 2-20ng/ml; spraying the suspension solution on the leaf surface of the tobacco after suspension solution is diluted 1000 times. The method has the advantages of simple and convenient production, low cost and no harm to human, animals and higher animals. The invention is suitable for massive use on tobaccos. The prepared suspension solution overcomes the defects of wettable powder and emulsion; accessory ingredient is easy to disperse. The invention is more convenient to apply in fields and has no remnant and environment pollution.

The invention relates to the technical field of biology and discloses a non-alcoholic chronic fatty liver hepatitis (NASH) rat model and an establishment method and application thereof. An adult rat is fed with special feed (MCD feed) short of methionine and choline or a special high-fat feed (CDAA feed) short of choline, a certain amount of sodium nitrite solution is provided for the animal within a certain time, and the established non-alcoholic chronic fatty liver hepatitis rat model has the characteristics of chronic liver fibrosis and earlier liver fibrosis. The rat model is simple in establishment process, a technical means is easy, the establishment cost is low, the rat model has very high animal model value for scientific research and research and development of drugs, especially serves as a specific animal model with NASH accompanied with severe liver hepatitis and earlier liver fibrosis and belongs to a national initiative.

To clarify histamine receptor H3 protein function in vivo, the present inventors constructed a nonhuman higher animal in which the expression of a histamine receptor H3 gene was artificially inhibited. As a result, the present inventors found that this nonhuman higher animal showed increased body weight, food intake, blood insulin level, or blood leptin level compared with a control. Thus, the present inventors found that abnormalities in the histamine receptor H3 protein relate to diseases characterized by changes in body weight or food intake, and this has made it possible to screen drugs for treatment or prevention of these diseases, and to examine these diseases.

The invention relates to a biological gene clone expression technology, and particularly relates to a method of rapidly and efficiently constructing an expression vector of a transcription activator-like effector nuclease (TALEN). The method is particularly suitable for constructing an expression vector aiming at higher animal genome genetic modification in large scale so as to study functions of animal genes.

The invention relates to a research on the prediction of artery pressure baroreflex sensitivity, BRS on dead brain, and the research of antihypertensive ketone selin on dead brain. Wherein, (1), BRS can be used as one independent factor to predict the occur of dead brain, that the occur time of BRS-high animal is later then the lower one; (2), ketone selin can effective improve BRS, without affected by its amount, to delay the dead brain and prolong life time.

The invention provides a double digital PCR (polymerase chain reaction) method of African struthio camelus derived ingredient quantitative detection. A two-channel detection method is used; a digitalPCR system is used; meanwhile, two kinds of fluorescence signals of African struthio camelus specific species gene and higher animal specific gene are detected; the sequence detection probes of the African struthio camelus specific species gene and the higher animal specific gene are respectively marked as FAM and VIC; the sequence copy number of the African struthio camelus specific species geneand the higher animal specific gene is measured through the same PCR system; the relative content of the African struthio camelus derived ingredients accounting for higher animal derived ingredients is obtained through calculation. The method has the advantage that the relative quantification on the copy number proportion of the African struthio camelus derived ingredients accounting for the totalmeat derived ingredients in meat food can be realized.

The invention relates to the technical field of medicines and particularly discloses a compound florfenicol preparation for aquatic animals and a preparation method of the compound florfenicol preparation for the aquatic animals. The compound florfenicol preparation for the aquatic animals is prepared from, by mass, 10-20% of florfenicol, 5-15% of acetylisovaleryltylosin tartrate, 10-20% of glucurolactone, 12-20% of 2-deoxyglucose and the balance auxiliary materials. The preparation method includes manufacturing steps: drying the auxiliary materials until moisture content is 8% or below, respectively subjecting auxiliary and raw materials to grinding and screening through a 80-mesh screen, and well mixing the screened powdery auxiliary and raw materials in a mixer. The compound florfenicolpreparation for aquatic animals is easy to produce, effective in treatment of bacterial diseases of the aquatic animals, low in drug resistance and effective in protection and repair livers of the aquatic animals. By formula and process optimization, problems of poor treatment effects, high animal liver burden and the like of antibacterial medicines are solved. The compound florfenicol preparation is suitable for aquatic animals and used for treatment of various bacterial diseases.

Disclosed herein are methods to assess the biological safety of an agent. The method involves contacting one or more test agents to a culture of C. elegans and analyzing the culture for meiotic disruption, wherein an increase in meiotic disruption of the C. elegans, indicates that the test agent(s) has reduced biological safety to mammals. An increase in meiotic disruption of the C. elegans also indicates a likelihood that the test agent is a reproductive toxicant in higher animals, such as humans. Also disclosed are methods to identifying disruptors of fat homeostasis in a mammal. The method involves contacting one or more test agents to a culture of C. elegans and analyzing the culture for fat content, wherein a change in the fat content, as compared to an appropriate control, indicates that the test agent(s) is a likely disruptor of mammalian fat homeostasis.

The invention discloses a production technology of saccharomyces cerevisiae fermented soybean meal. The production technology comprises the following steps: (1) saccharomyces cerevisiae strain activation; (2) saccharomyces cerevisiae strain liquid culture; (3) saccharomyces cerevisiae strain triangular flask culture; and (4) soybean meal fermentation. The production technology is simple and easy to understand, and the produced soybean meal has the advantages of high crude protein content, reasonable amino acid composition, high animal digestion utilization rate and the like, saccharomyces cerevisiae used in livestock production can save costs, reduce morbidity, and improve the economic benefit, and is helpful to obtain high-quality livestock products.

The invention provides a dual digital PCR (Polymerase Chain Reaction) method for quantitatively detecting turkey derived ingredients. The dual digital PCR method comprises the following steps: simultaneously detecting two fluorescence signals of a turkey specific species gene and a higher animal specific gene by adopting a dual-channel detection method and utilizing a digital PCR system, respectively marking probes for sequence detection of the turkey specific species gene and the higher animal specific gene as FAM and VIC, and calculating relative content of the turkey derived ingredients inhigh animal derived ingredients through copy numbers, which are detected in the same PCR system, of sequences of the turkey specific species gene and the higher animal specific gene. According to thedual digital PCR method provided by the invention, a ratio of the copy number of the turkey derived ingredients in total meat derived ingredients of meat food can be relatively quantified.

The invention relates to bis-biguanide, a preparation method thereof and an application of the bis-biguanide. The bis-biguanide has the advantages that a novel anti-irradiated injury series compound is designed and synthesized, the novel compound which is high-efficiency and low-toxicity is found out and can be used for anti-irradiated injury in the field of civil use and military, by the novel compound, hematopoietic function injury caused by radiation or chemotherapy drugs is reduced, and blood cellular level is increased, or tumor auxiliary treatment is implemented; and a molecular level and animal pharmacological activity test is performed by the compound, and a result shows that the bis-biguanide has high in-vitro activity and high animal treatment effect generally.

Belonging to the feed processing field, the invention relates to insect rainbow trout feed, especially a preparation method of rainbow trout feed with insects as raw materials. The feed is characterized by comprising, by weight: 15-20% of Tenebrio molitor, 15-20% of termites, 15-20% of fly maggots, 15-20% of silkworm chrysalises, 15-20% of eggs, 3-6.5% of prosomillet, 2-5% of alga powder, 4-8% of soybean oil, 2-3% of white radishes, 2-8% of radish seeds, and 2-8% of garlic. The feed of the invention has the advantages of high animal protein content, comprehensive nutrition necessary for promoting rainbow trout growth, strong feeding attraction, high digestibility, gut immunity improvement, virus and disease prevention, good stability in water, difficult pollution to aquaculture water quality, no peculiar smell, no organic residual, better application security, and broader application prospects.

The invention provides a plant for controlling damage of fall webworms and a simple environmental application method of the plant to urban greening trees. The simple environmental application method of the plant to urban greening trees is characterized by including: cutting harvested whole plants of Artemisia lavandulaefolia into 2-3cm long pieces, placing into a vessel, adding 1-2% of edible sugared water, and mixing well; and when Artemisia lavandulaefolia leave the trees to overwinter each year, mixing and grinding the Artemisia lavandulaefolia with the edible sugared water according to a ratio of 2:1, scattering mixed Artemisia lavandulaefolia at the position of 20-30cm around each shade tree, covering all of Artemisia lavandulaefolia with earth once every 5-7 days, and continuously covering for two to three times. By the method, pupation rate of fall webworms is lowered by 60-75%. The plant and the method have the advantages that the natural resource Artemisia lavandulaefolia of China is fully utilized, the plants of Artemisia lavandulaefolia grow massively around China and appear everywhere, obtaining Artemisia lavandulaefolia is simple and easy, and accordingly waste can be turned into wealth; and Artemisia lavandulaefolia is nontoxic to higher animals and beneficial lives, the application method of Artemisia lavandulaefolia to urban greening trees is simple and easy, the cost of the method is about one twentieth of that of the existing methods, evident economic benefit is achieved, and great ecological benefit and social benefit can also be achieved.