Method of achieving protein expression vector capable of genome site-specific modification

Abstract

The invention relates to a biological gene clone expression technology, and particularly relates to a method of rapidly and efficiently constructing an expression vector of a transcription activator-like effector nuclease (TALEN). The method is particularly suitable for constructing an expression vector aiming at higher animal genome genetic modification in large scale so as to study functions of animal genes.

Description

technical field [0001] The invention relates to biological gene cloning and expression technology, in particular to a method for quickly and efficiently constructing a transcription activator-like effector protein cutting enzyme (TALEN) expression vector. It is especially suitable for large-scale construction of expression vectors for genetic modification of higher animal genomes to study the functions of animal genes. Background technique [0002] one. Introduction and application of TALEN technology [0003] With the completion of the whole genome sequence determination of more and more species, the current genomics research hotspot has shifted from structural genomics to functional genomics, so the development of fast, efficient, high-throughput gene function research methods and strategies have a wide range of needs. Gene knockout (Knockout) technology is the most important method in the study of animal gene function. In the past year or so, there have been reports i...

AI Technical Summary

Problems solved by technology

[0007] two. Difficulties in the implementation of TALEN technology

[0013] 2. High cost

The biggest disadvantage of chemical synthesis methods at this stage is that the cost of chemically synthesizing long DNA fragments is very high

According to the current product synthesis price of major suppliers, the cost of synthesizing an expression element DNA with a DNA binding domain containing 20 repeat units is higher than RMB 20,000-30,000

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