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Clone for pork generation character related gene BTG1 of pig and application thereof in pig molecule mark auxiliary selection

A molecular marker-assisted, production trait technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as the function of pig BTG1 gene that have not been studied.

Inactive Publication Date: 2008-03-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is still no report on the study of the function of the pig BTG1 gene

Method used

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  • Clone for pork generation character related gene BTG1 of pig and application thereof in pig molecule mark auxiliary selection
  • Clone for pork generation character related gene BTG1 of pig and application thereof in pig molecule mark auxiliary selection
  • Clone for pork generation character related gene BTG1 of pig and application thereof in pig molecule mark auxiliary selection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: (1) Application of distribution and detection of PCR-RFLP-PsuI polymorphism in various pig breeds

[0056] Seven pig breeds were detected by PCR-PsuI-RFLP: including foreign pig breeds from European bloodlines (the same below): Large White, Landrace and Duroc pigs, and breeds from Chinese local pig bloodlines (the same below): Tong City pigs, Erhualian pigs, Qingping pigs and Jiangxi Yushan pigs, these pig breeds basically cover the genotypes of various pig breeds. The gene frequencies of the pig breeds used in the experiment are shown in Table 3. It was found that the allele A was dominant in Landrace, Large White, Tongcheng, Erhualian and Qingping pigs, and the allele T was dominant in Duroc and Jiangxi Yushan pigs.

[0057] Table 3 Detection of BTG1 gene PCR-RFLP-PsuI genotype frequency and gene frequency

[0058] pig breed

[0059] The chi-square test results of genotype frequencies among several varieties are shown in Table 4. x 2 The tes...

Embodiment 2

[0071] Example 2: Application Example of Pig Marker-Trait Association Analysis

[0072] BTG1 gene was used to detect 179 pigs in the Tongcheng test group, and the three genotypes detected by the polymorphism of the amplified sequence PsuI-RFLP in the 3'-UTR region of the gene were initially correlated with some production traits of the Tongcheng test group . The results of genotype detection showed that among 179 individuals, there were 144 individuals with AA genotype, 33 individuals with AT genotype, and 2 individuals with TT genotype. The analysis results showed that the genotype of this locus was significantly correlated with shoulder fat, 6-7 rib fat thickness, slaughter rate and meat color. The shoulder fat of AT type was significantly higher than that of homozygous genotypes AA and TT; the fat thickness of AT type 6-7 was significantly higher than that of TT type; the slaughter rate of TT type was significantly higher than that of AA type; the meat color of AA type was...

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Abstract

The present invention belongs to the field of animal gene technology, and is especially one pig gene BTG1 related to its pork producing character and its cloning and application in auxiliary selection of pig's molecular marker. The present invention clones pig gene BTG1 related to its pork producing character with the sequence of SEQ ID No. 1, which has one A179-T179 base mutation in No. 179 bp and one C280-T280 base mutation in No. 280 bp to result in Psu1-RFLP polymorphism and Bsh1236I-RFLP polymorphism separately. The present invention also discloses the preparation process of the said gene and its primer, and provides new molecular marker for auxiliary selection of pig.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to the cloning of a pig production trait-related gene BTG1 gene and its application in pig molecular marker-assisted selection. Background technique [0002] Since ancient times, pigs have always been the first of the six animals in my country. The ups and downs of the pig industry affect the improvement of people's living standards. Worldwide consumption of pork accounts for 43% of total red meat consumption, and pigs are an important model system for the human body as well as an important source of future organ transplants (Rothschild. From a sow's ear to a silk purse: real progress in porcine genomics. Cytogenetic and Genome Research. 2003, 102:95-99). Therefore, the research on pigs has important practical significance no matter from the point of view of national economy or from the point of view of human health. [0003] my country is one of th...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12Q1/68
Inventor 赵书红冯政沈鹤李奎余梅朱猛进李长春樊斌刘榜
Owner HUAZHONG AGRI UNIV
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