105results about How to "Increase secretion level" patented technology

Recombinant photosynthetic microorganisms that convert inorganic carbon to secreted fatty acids are described. Methods to recover the secreted fatty acids from the culture medium without the need for cell harvesting are also described.

The invention relates to an interference sequence-modified TGF-beta1 silent leukemia cell exosome and a preparation method and application thereof. Through expression of TGF-beta1 of a lentivirus vector silent exosome source cell with shRNA, the leukemia cell exogenic exosome, namely LEXTGF-beta1si of which the TGF-beta1 content is regulated down is produced and purified. The transformed leukemia cell exogenic exosome-sensitized dendritic cell is prepared into a DCLEX-TGF-beta1si vaccine. An in vitro and animal model experiment shows that the transformed vaccine is capable of generating more powerful specific anti-leukemia immunoreaction.

The invention relates to a traditional Chinese medicine composition for preventing and treating salpingitis of a laying hen. The traditional Chinese medicine composition is an oral solution prepared from codonopsis pilosula, rhizoma atractylodis macrocephalae, angelica sinensis, ligusticum wallichii, astragalus membranaceus, cortex acanthopanacis, radix rehmanniae preparata, cinnamon, peach kernel, flos carthami and agrimonia pilosa ledeb. The composition is prepared by the following steps: (1) grinding the traditional Chinese medicinal materials to 20-60 meshes, weighing according to the prescribed amount, and mixing in a three-dimensional manner to obtain traditional Chinese medicine powder; (2) putting the mixed traditional Chinese medicine powder into an extraction pot, adding 100-200 parts of water, and decocting for 2-12 hours; (3) filtering to remove decoction dregs, and keeping filtrate for later use; (4) putting the decoction dregs into the extraction pot once again, adding 100-200 parts of water, and decocting for 2-12 hours; (5) filtering to remove decoction dregs, and combining liquid and the first extracted liquid; (6) concentrating at 30-100 DEG C in reduced pressure until the volume of the concentrate is 10-50 percent of the original volume, filtering to remove dregs, and filling into bottles (250ml / bottle). The traditional Chinese medicine composition is capable of effectively preventing and treating salpingitis of the laying hen, has a cure rate of over 90 percent, and has the effects of nourishing qi and blood, activating blood circulation to remove stasis, astringing and stopping bleeding, and nourishing yin and warming kidney.

An expression construct is disclosed which is useful for delivering an exogenous polypeptide (e.g. a growth hormone such as somatotropin) to a host. In one application of the invention, the expression construct is introduced into a non-host recombinant cell encapsulated in a semi-permeable membrane for implantation into the host. The semi-permeable membrane inhibits immune surveillance and cell rejection events so that non-host, highly expressing, recombinant cells can be used.

The invention relates to a formula of a health-care product, namely, garlic oil and sea buckthorn oil containing soft capsules, and a preparation process. The garlic oil and sea buckthorn oil containing soft capsules are obtained through research on the basis of existing sea buckthorn oil type soft capsule health care food. According to the formula, the soft capsules are prepared from 20-100 parts of garlic oil, 50-500 parts of sea buckthorn oil, 200-800 parts of corn oil, 100-200 parts of gelatin, 50-100 parts of glycerin, 100-200 parts of purified water, 1-2 parts of titanium dioxide and 0.5-5 parts of radish red through processes of burdening, drying, pressing and the like and have the efficacy of boosting the immunity and the like.

Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.

The invention relates to a self-assembly nanometer adjuvant and a preparing method and application of nanometer vaccine formed by the self-assembly nanometer adjuvant. According to the self-assembly nanometer adjuvant and the preparing method and application of the nanometer vaccine formed by the self-assembly nanometer adjuvant, protamine sulfate and carboxymethyl dextran serve as self-assembly materials, the protamine sulfate, the carboxymethyl dextran and CpG oligodeoxynucleotide form the nanometer adjuvant in a self-assembly manner, and the nanometer vaccine is formed by the nanometer adjuvant and a viral antigen. Through the nanometer adjuvant, the bioavailability of the CpG oligodeoxynucleotide is improved, it is avoided that the CpG oligodeoxynucleotide is degraded in the body, andfunctions of A-type CpG are increased through B-type CpG. Through the nanometer vaccine, a TH1-type humoral immunity response can be induced, and meanwhile a high cellular immune response can be induced. Mice in-vivo challenge experiments verify that the nanometer vaccine has a good protection effect, and provides help for future nanometer-vaccine application.

The invention relates to application of cystic fibrosis transmembrane transduction regulating factor inhibitor to the preparation of medicaments for treating diabetes, and application of thiazolidone compounds or derivatives thereof to the preparation of medicaments for treating diabetes. The cystic fibrosis transmembrane transduction regulating factor inhibitor can be subjected to compatibility with medicaments of sulfonylurea, glinides, biguanides, insulin sensitizers and glycosidase inhibitors.

The invention discloses application of a polyinosinic-polycytidylic acid (poly I:C) combined dimo-thylidioctyl ammonium bromide (DDA) mixed adjuvant to the preparation of tuberculosis subunit vaccines. The invention has the advantages that: the mixed adjuvant DDA / Poly I:C can enhance the secretion level of antigenic specificity interferon-gamma (IFN-gamma) of fusion protein Ag85B-MPT64190-198-Mtb8.4 (AMM) effectively and relieve pathological damage caused by H37Rv infection, and is an effective adjuvant of vaccines for preventing tuberculosis.

Cell-associated secretion-enhancing fusion proteins are disclosed that comprise a target protein binding domain and a transmembrane retention domain. Co-expression in a host cell of a fusion protein and a target protein of interest that is temporarily bound by the fusion protein leads to an increased level of target protein secreted from the host cell. The fusion proteins are engineered to be retained with the producing host cell, thus eliminating a non-natural component from the extracellular media of the host cell and simplifying purification of the target protein. Nucleic acid molecules encoding such fusion proteins are also disclosed for use in expressing the fusion proteins in host cells, for use in restoring lost or diminished cell functions, and for use in treating diseases characterized by a lost or diminished cell function. Methods and compositions comprising fusion proteins of the invention are disclosed for use in enhancing the level of co-expressed target proteins secreted from host cells.