The invention discloses health-caring walnut sour-milk and a preparation method thereof. The health-caring walnut sour-milk is prepared from the following raw materials: walnut kernels, milk powder, oat flour, bran powder, buckwheat flour, corn oligopeptides, carrot powder, pumpkin powder, white granulated sugar, sugar alcohol, oligosaccharides, lily bulb powder, and honeysuckle flower powder. Thepreparation method of the health-caring walnut sour-milk comprises the following steps: A, performing pretreatment on raw materials, namely preparing walnut milk from carefully selected high-qualitywalnuts; B, blending raw-material milk and carrying out homogenization, namely adding other ingredients into the walnut milk, carrying out stirring so as to have the raw-material milk uniformly mixed,and carrying out homogenization for two times; C, carrying out sterilizing and cooling; D, carrying out inoculation and fermentation, namely adding domesticated sour milk fermentation agent into thesterilized and cooled milk liquid, wherein the addition amount of the domesticated sour milk fermentation agent in mass ratio is 2.5-3.5%, and carrying out fermentation at 42-44 DEG C for 5-7 hours; and E, carrying out refrigerated post-ripening, namely rapidly taking the fermented sour milk out after completion of the fermentation, and carrying out cooling. By adding a plurality of dietary fibers, the health-caring walnut sour-milk disclosed by the invention has significant health-caring functions; moreover, no preservative is added during the preparation process, so that, the health-caring walnut sour-milk is a natural nutritious health-caring food. And thus, the concept for healthy life of the modern people is met.