The invention discloses an edible natural propolis antibacterial toothpaste and a preparation method thereof. The weight ratio of raw materials is: 0.2-0.8% of propolis extract, 15-20% of friction agent, 50-70% of moisturizing agent, and 0.5% of binder ~1.0%, foaming agent 2.0~3.0%, sweetener 0.1~0.3%, conditioner 0.5~3.5%, preservative 0.1~0.3%, essence 1.0~1.2%, deionized water balance. This toothpaste utilizes the natural antibacterial and antiseptic properties of propolis, is non-toxic and non-irritating, and can be used in toothpaste instead of triclosan, formaldehyde, sodium benzoate, paraben, etc., reducing the use of synthetic antibacterial agents, and has a good antibacterial effect , and avoid potential safety hazards.