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37results about How to "Solve reproductive problems" patented technology

Fence preservation method for prolonging shelf life of cold fresh poultry

The invention discloses a fence preservation method for prolonging shelf life of cold fresh poultry. The fence preservation method for prolonging the shelf life of the cold fresh poultry comprises the following steps of: placing fresh poultry which is obtained by slaughtering, washed completely and cut into a refrigeration storage at the temperature of between 2 and 5 DEG C and precooling the poultry for 1 to 2 hours, so that the surface temperature of the poultry is reduced below 5 DEG C and the internal temperature of the poultry is reduced below 10 DEG C; transferring the precooled poultry to a purification room and performing surface sterilization on the poultry through ozone or ozone solution of an ozone generator; immersing the poultry subjected to ozone sterilization into chitosan glutamate solution to retain freshness; taking out and draining the poultry; placing the poultry after corrosion prevention and freshness preservation into a food plastic packaging box; introducing carbon dioxide (CO2) and nitrogen (N2) into the food plastic packaging box and performing sealing package; and storing the packaged poultry into a cold chain at the temperature of between 2 and 5 DEG C. The fence preservation method for prolonging the shelf life of the cold fresh poultry has good effect and is suitable for retaining freshness of the fresh cut poultry.
Owner:HUAIYIN INSTITUTE OF TECHNOLOGY

Application of water soluble chitosan in toothpaste and mouthwash

The invention discloses an application of soluble chitose as oral article, which is characterized by the following: sterilizing Gram-positive bacterium and negative bacterium due to 0.1-20% soluble chitose; possessing affinity with oral bacteria and oil anion; repairing ulcer; accelerating microcirculation of surface capillary; augumenting the response of immune cell in the deep layer.
Owner:王立君

Method for cutting, breeding and seedling of Wild Vitis quinquangularis in Guangxi Province

InactiveCN101273692ASolve reproductive problemsLow costCultivating equipmentsSoilless cultivationDowny grapeVitis quinquangularis
The invention relates to a method for the propagation by cuttings and culture of seedling of Guangxi wild downy grape, and the propagation by cuttings is adopted, perlite, peat and sand are mixed uniformly according the volume ratio of 1:1:1 to be used as the substrate, wherein, the relative humidity of the substrate is 60 to 80 percent and the thickness of the substrate laid on an electric blanket is 10 to 15cm and a plastic film is covered on the substrate; the length of the cutting of the wild downy grape is 15 to 20cm, the base of the cutting is treated with a root-inducing regulator, the part of the cutting exposed to the air is wrapped up by the plastic film to be inserted on the substrate; the temperature of the substrate is controlled at 30 DEG C at night, and 20 DEG C in the daytime. The technique of the invention has the prominent advantages of low cost, simple operation and easy promotion, with the cutting survival rate of more than 70 percent.
Owner:GUANGXI UNIV

Method for generating and cultivating papuan jellyfish ephyrae

InactiveCN105104246AReproduce fastFill in the breeding gapClimate change adaptationPisciculture and aquariaArtificial rearingTransverse cracking
The invention discloses a method for generating and cultivating papuan jellyfish ephyrae and belongs to the field of artificial feeding and breeding of ornamental marine animals. A papuan jellyfish hydranth transversely cracks to generate an ephyra every time, the ephyra is continuously cultivated for 5 to 10 days, and second-time single-ephyra transverse cracking is generated; the ephyra which just cracks is bred in a container where bubbles drive water flow, one to three artemia nauplii are fed with each milliliter of water every day, and the water is changed after the artemia nauplii are fed for 2h. Cultivation conditions are as follows: the water temperature is 20 to 27 DEG C, 8000Lux 8h illumination 16h dark alternation is carried out, 5 to 10d ephyrae are transformed into jellyfish bred bodies and the jellyfish bred bodies are moved into a circulation cylinder where a water pump drives the water flow. According to the method, transverse cracking of the papuan jellyfish hydranth is promoted in an artificial environment, the ephyrae are generated and are further cultivated to obtain the jellyfish bred bodies and adults, and the situation that exhibition in an aquarium is maintained by virtue of fishing is solved.
Owner:青岛海洋科技馆

Male fertility maintaining method and application thereof

The invention discloses a wheat fertility recovery gene and an application thereof, belongs to the field of plant biotechnology, and in particular relates to a recessive genic male sterility gene and cloning of a promoter of the gene as well as an application of the gene in crossbreeding. Through flow cytometry and a high-throughput sequencing method, the fertility recovery gene FRG1 is successfully cloned; and with the application of the gene, male fertility of a Lanzhou genic male sterile mutant or an allelic mutant thereof can be completely recovered, so that a foundation is laid for the construction of a novel wheat crossbreeding technique system. Meanwhile, more possibilities are offered for solving such technical bottleneck problems in existing 'three-line' and 'two-line' wheat crossbreeding technologies which are unstable in sterile line fertility, limited in resource of hybrid varieties, complex in seed production technique, high in seed production cost and the like. The gene and sterile line propagation and maintaining method provided by the invention has great significance and application value of wheat crossbreeding work.
Owner:BEIJING NEXT GENERATION HYBRID WHEAT BIOTECHNOLOGY CO LTD +1

Method for cutting and raising seedlings of tutcheria hexalocularia employing container

The invention discloses a method for cutting and raising seedlings of tutcheria hexalocularia employing a container. An offspring plant container seedling which is kept consistent with an excellent single strain in inheritable characters is obtained in manners of selecting an excellent single strain of the tutcheria hexalocularia as a stock plant by an inventor; selecting a nursery land, and preparing soil; preparing and sterilizing matrix; collecting, cutting and processing cuttings; moisturizing, shading, managing after rooting, and the like. Blooming is earlier than sowing seedling, and the cutting survival rate and the transplanting survival rate are high. The method disclosed by the invention is simple to operate, low in production cost and applicable to vegetative propagation of excellent single strain of the tutcheria hexalocularia; the difficulties of propagating and transplanting of the excellent single strain of the tutcheria hexalocularia can be effectively solved; the method can be applied to massive cultivation of seedlings of rare plants and excellent ornamental tree tutcheria hexalocularia; the difficulty of seedling of the key protected wild plant, namely the tutcheria hexalocularia in Guangxi can be broken through; protection of the rare plant tutcheria hexalocularia resource, and application and promotion thereof in landscaping are facilitated.
Owner:GUANGXI FORESTRY RES INST

Production method of weather-resistance nutritional lick block for promoting reproduction of cows

The invention relates to a weather-resistance nutritional lick block for promoting the reproduction of cows. The main manner of cow breeding in China still takes a decentralized mode as the main part, and a decentralized breeding system can not support the high-cost daily ration with overhead price, so that dairy cows or meat cows always have breeding disorder and other nutritional and metabolic diseases. Experiences and experiences abroad prove that the feed supplementing lick block is an economical and effective technical approach under the condition. At present, the manufacturing technology of the special feed form, namely the lick block, in China is not mature. Particularly, the weather-resistance of the lick block is poor, so that the lick block can split after being blown by wind and wetted by rain, and the hardness of the lick block is reduced greatly; in addition, on the aspect of formula technology, the influential formula for preventing the breeding disorder for the cows does not exist. Therefore, the application and the promotion of the lick block in the cow breeding industry in China are limited greatly. The invention provides a formula of the weather-resistance type lick block for promoting the reproduction of the cows, and a preparation process thereof. As for the lick block, the weather resistance of the lick block is limited and improved, the reproduction performance of the dairy cows and the meat cows under the decentralized mode is improved by utilizing the special formula, and parorexia and other nutritional and metabolic diseases can be prevented and treated.
Owner:SHENYANG AGRI UNIV

Hemotrophin and chorionic gonadotrophin composite hormone freeze-dried powder and preparation method thereof

The invention belongs to the technical field of veterinary drugs, and particularly relates to hematotrophin and chorionic gonadotrophin composite hormone freeze-dried powder and a preparation method thereof. The hematotrophin and chorionic gonadotrophin composite hormone freeze-dried powder is prepared from the following raw materials: hematotrophin, chorionic gonadotrophin, a stabilizer, an excipient, a buffering agent and water. The inventor finds that the hematotrophin and the chorionic gonadotrophin are compounded to have a synergistic effect, and according to the technical scheme, after the hematotrophin and the chorionic gonadotrophin are dissolved and uniformly mixed according to a certain proportion, the biological activity and the stability of the hematotrophin and the chorionic gonadotrophin can be maintained to the maximum extent by adjusting parameters in a freeze drying technology; and the preservation time is prolonged, and no side effect or potential safety hazard exists. Meanwhile, the action effect of the compound hormone is greatly improved, and the breeding problem of female livestock or male livestock is well solved.
Owner:浙江科瑞特生物科技有限公司 +2

Use of freshly squeezed red bayberry juice in killing of escherichia coli

The invention discloses a method for killing escherichia coli and especially discloses a use of a freshly squeezed red bayberry juice in killing of escherichia coli. According to the invention, a freshly squeezed red bayberry juice is added into a liquid containing escherichia coli, and the mixture is uniformly mixed and then is subjected to pressure maintaining treatment under the pressure greater than or equal to 10MPa for 1-1.5min, wherein the volume of the freshly squeezed red bayberry juice is at least 1 time volume of the liquid containing escherichia coli. The method provided by the invention can greatly reduce pressure requirements under high-pressure sterilization conditions and avoid microbe follow-up breeding in a storage period after sterilization.
Owner:ZHEJIANG UNIV

Brachyceras overwintering storage and propagation method

ActiveCN106105588ASolve technical problems of overwintering storagePracticalAgriculture tools and machinesSeed and root treatmentFrostLandscape architecture
The invention belongs to the technical field of plant storage and discloses a brachyceras overwintering storage and propagation method. The method includes the steps of picking of dormant stems, wherein one week after arrival of first frost, brachyceras plants growing in the open air are dug out, leaves and root systems on the plants are removed, the formed dormant stems are acquired, and soil and impurities on the dormant stems are flushed away with clear water; air-drying of the dormant stems, wherein the dormant stems are arranged at indoor shady, cool and ventilating positions and dried in the shade till the surfaces are dry; storage of the dormant stems, wherein the air-dried dormant stems are put in plastic self-sealing bags, bag openings are sealed, and the dormant stems are subjected to overwintering storage under the light-shading and indoor 4.5-16 DEG C temperature varying conditions. The invention further relates to a method for propagating brachyceras through dormant balls. The method facilitates material obtaining, is easy to operate, saves cost, solves the problem about overwintering storage of the brachyceras in the northern areas of China, and provides experimental bases for further application and popularization of the brachyceras in northern landscape architecture.
Owner:XIAN BOTANICAL GARDEN SHAANXI PROV

Method for rapidly propagating roots of subprostrate sophora by tissue culture

The invention relates to a method for rapidly propagating roots of subprostrate sophora by tissue culture. The method provided by the invention comprises the following steps of: (1) taking seeds of the roots of subprostrate sophora as explants to be disinfected; (2) putting the disinfected explants into an MS (Murashige and Skoog) basic culture medium to be induced and germinated to obtain sterile test-tube seedlings; (3) putting the sterile test-tube seedlings into an MS propagating culture medium to carry out a rapid propagating culture on the sterile test-tube seedlings to obtain cluster buds; (4) putting the cluster buds into an MS sound seedling culture medium to carry out a sound seedling culture to obtain strong plants; (5) putting the strong plants into an MS rooting culture medium to be cultured to obtain complete seedlings with roots; and (6) taking the complete seedlings with roots to adopt seedling adaptation and then transplanting the seedlings to a sand bed to grow for one month; and then transplanting the seedlings to a land for growing field crops. The germchit obtained by the method is strong, has a high survival rate, and can supply a lot of good-quality germchits of the roots of subprostrate sophora in short order; and the method effectively solves the problem of a large-scale seedling culture of the roots of subprostrate sophora.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Method for screening Bacillus thuringiensis nematocidal strain

The process of screening nematocidal Bacillus thuringiensis strain includes the steps of soaking bioassay, culture medium bioassay, protein crystal bioassay, etc. The process adopts easy-to-obtain defect type colibacillus JM109, and has short screening period, low cost, high screening efficiency. The screened nematocidal Bacillus thuringiensis strain IPS has a 24 hr LC50 of 1.008 mcg / ml.
Owner:FUJIAN AGRI & FORESTRY UNIV

Application of freshly squeezed bayberry juice to bacillus subtilis killing

The invention relates to a killing method of bacillus subtilis, in particular to an application of freshly squeezed bayberry juice to bacillus subtilis killing. The application comprises the following steps: first, adding freshly squeezed bayberry juice in a liquid containing bacillus subtilis, and carrying out pressure maintaining treatment for 1-1.5 minutes under the high pressure higher than or equal to 10 MPa after uniformly mixing, wherein the freshly squeezed bayberry juice is at least twice of the volume dose of the liquid containing bacillus subtilis. Through the adoption of the application, the pressure requirement of high-pressure bacteria killing condition can be reduced greatly, and the continuous propagation during the microorganism storage period after bacteria killing is avoided.
Owner:ZHEJIANG UNIV

Rapid propagation method for hemp seeds

The invention discloses a rapid propagation method for hemp seeds. The method includes the following steps that the screened hemp seeds are put into 50% carbendazim wettable powder with the concentration of 0.2% to be subjected to immersion disinfection, and moisture on the hemp seeds is drained off; the hemp seeds are immersed in 30-35 DEG C warm water for 18-24 h, and moisture on the hemp seeds is drained off; 50-70-mesh perlite is taken and heated for 10-15 min in a 150-160 DEG C drying oven and then naturally cooled, clean water is added to wet the perlite, and treated perlite is obtained; the treated perlite is laid in a box with holes in the bottom, the immersed hemp seeds are evenly scattered on the perlite, and another layer of perlite is laid; the box is irradiated for 10-24 h every day through an infrared thermal insulation lamp and placed in a 28-30 DEG C environment in the remaining time every day, and watering is performed once every day. The method is easy and fast to implement and low in cost, has great economic benefits and social benefits and provides reliable guarantees for large-scale industrial planting of the hemp seeds.
Owner:广西巴马常春藤生命科技发展有限公司

Method for synergistically killing staphylococcus aureus through citruse naringenin and high-strength pulsed electric field

The invention discloses a method for synergistically killing staphylococcus aureus through citruse naringenin and a high-strength pulsed electric field. The method comprises the following steps of adding naringenin to a liquid food containing staphylococcus aureus, to be sterilized, and performing pretreatment; and preforming sterilization on materials pretreated with the naringenin by the high-strength pulsed electric field. Preferably, the addition concentration of the naringenin is 12.5-125mg / L, the pretreatment time is 0.5-1.0h, the parameters of the pulsed electric field are set as that the electric field strength is 20-50kV / cm, the pulse width is 10-40[mu]s, the pulse frequency is 0.1-1.0kHz, and the total time that materials are subjected to pulsing treatment is 60-1000[mu]s. According to the method disclosed by the invention, a natural antibacterial ingredient namely the naringenin is combined with a high-strength pulsed electric field sterilization technique, so that the effect of killing the staphylococcus aureus is notably increased, the food is safe, the storage period is prolonged, and the loss of original flavor and original nutrient substances is reduced.
Owner:SOUTH CHINA UNIV OF TECH

Ostrich feed using whole plant wheat as major raw materials

The invention relates to ostrich feed using whole plant wheat as major raw materials. The ostrich feed is prepared from the following raw materials in percentage by weight: 11 to 16.7 percent of corn,0 to 6 percent of corn protein power, 60 to 70 percent of whole plant wheat, 3 to 15 percent of rapeseed meal, 6 to 10 percent of cotton meal, 0.8 to 3 percent of bean oil, 1 percent of 1-percent poultry premixed materials, 0.2 to 6 percent of stone powder, 1 to 1.1 percent of calcium hydrophosphate, 0.1 to 0.25 percent of sodium chloride premixed materials, 0.1 percent of choline, 0 to 0.3 percent of lysine, 0 to 0.2 percent of methionine and 0.15 to 0.3 percent of sodium bicarbonate. The whole plant wheat is obtained by crushing overground parts harvested after the wheat is matured and naturally dried in the land and the seed moisture is lower than 20 percent to a state that all materials can pass through a sieve being 60 to 80 meshes. The ostrich feed provided by the invention has theadvantages that the whole plant wheat accounts for 60 to 70 percent of the feed; the bean meal and the wheat bran are completely replaced; the additional addition of straw powder is not needed; the maximum consumption of the corn is only 16.7 percent; the requirements of the ostriches can be met only through the matching of a small amount of mixed meal; the feeding cost is lower; the nutrition level can be balanced; the ostrich physique can be improved; the egg yield and the hatching rate can be obviously improved.
Owner:张宏芳

Plant pollen tube growth gene and application

ActiveCN112239762ASolving sterile reproductive problemsSolve reproductive problemsPlant peptidesFermentationBiotechnologyNucleotide
The invention discloses a rice pollen tube growth related gene OsPTD1 (Pollen Tube Device) and application. Protein encoded by the gene is an amino acid sequence shown as SEQ ID NO.1, or protein whichhas the same function as OsPTD1 protein and is obtained by substituting, deleting or adding at least one amino acid; and a nucleotide sequence of the protein composed of the amino acid sequence shownas SEQ ID NO.1 is shown as SEQ ID NO.2. The OsPTD1 gene encodes ascorbic acid oxidase is an essential gene for normal growth of a pollen tube, abnormal functions of the gene can cause early rupture of the pollen tube, insemination cannot be completed, and gametophyte male sterility is generated; and the gene can be used for preparing gametophyte male sterility, and plays an important role in preventing transgenosis from drifting along with pollen, bred sporophyte male genic male sterility and the like.
Owner:YUNNAN UNIV

Artificial seedling raising method for black rosewood

The invention provides an artificial seedling raising method for black rosewood. The method comprises the steps of seed treating, wherein seeds are collected, seed treating liquid is prepared, the black rosewood seeds, the seed treating liquid and sandy soil are mixed to be uniform and laid in a seed culture pot, the seed culture pot is put in a greenhouse, germination accelerating is conducted, and black rosewood bud seedlings are obtained; land selecting for nursery land building, wherein nursery land is selected and treated; bud seedling transplanting; seedling stage managing, wherein seedling stage managing is conducted after bud seedling transplanting, and sunshading managing, water managing, fertilization managing, weeding managing and disease and pest preventing and controlling are conducted. According to the method, the high survival rate of black rosewood seed seedling raising is achieved, and the black rosewood planting difficulty is reduced; meanwhile, the condition that in a traditional planting mode, black rosewood nursery stocks are nonuniform and irregular is improved, and the nursery stock quality is improved.
Owner:HUIZHOU DONGJIANG LANDSCAPE ENG

Foamless sterilization remover for air separation recirculated cooling water

The invention discloses a foamless sterilization remover for air separation recirculated cooling water. The above water treatment agent comprises 2-20% of acrylic acid-acrylamido-2-methylpropanesulfonic acid-acrylate multipolymer, 5-30% of polyquatemary ammonium salt, 2-20% of sodium polyepoxysuccinate, 5-30% of polyhexamethylene guanidine and 10-50% of water. The water treatment agent can solve the problems of microbe breeding and biological slime deposition of air separation and oxygen production systems in order to effectively overcome insufficiencies of existing water treatment technologies and realize the energy saving and emission reduction purposes.
Owner:JIANGSU JIANGHAI CHEM

Method for cutting, breeding and seedling of Wild Vitis quinquangularis in Guangxi Province

The invention relates to a method for the propagation by cuttings and culture of seedling of Guangxi wild downy grape, and the propagation by cuttings is adopted, perlite, peat and sand are mixed uniformly according the volume ratio of 1:1:1 to be used as the substrate, wherein, the relative humidity of the substrate is 60 to 80 percent and the thickness of the substrate laid on an electric blanket is 10 to 15cm and a plastic film is covered on the substrate; the length of the cutting of the wild downy grape is 15 to 20cm, the base of the cutting is treated with a root-inducing regulator, thepart of the cutting exposed to the air is wrapped up by the plastic film to be inserted on the substrate; the temperature of the substrate is controlled at 30 DEG C at night, and 20 DEG C in the daytime. The technique of the invention has the prominent advantages of low cost, simple operation and easy promotion, with the cutting survival rate of more than 70 percent.
Owner:GUANGXI UNIV

Method for growing seedlings of six-petal stone pencil wood container cuttings

The invention discloses a method for cutting and raising seedlings of tutcheria hexalocularia employing a container. An offspring plant container seedling which is kept consistent with an excellent single strain in inheritable characters is obtained in manners of selecting an excellent single strain of the tutcheria hexalocularia as a stock plant by an inventor; selecting a nursery land, and preparing soil; preparing and sterilizing matrix; collecting, cutting and processing cuttings; moisturizing, shading, managing after rooting, and the like. Blooming is earlier than sowing seedling, and the cutting survival rate and the transplanting survival rate are high. The method disclosed by the invention is simple to operate, low in production cost and applicable to vegetative propagation of excellent single strain of the tutcheria hexalocularia; the difficulties of propagating and transplanting of the excellent single strain of the tutcheria hexalocularia can be effectively solved; the method can be applied to massive cultivation of seedlings of rare plants and excellent ornamental tree tutcheria hexalocularia; the difficulty of seedling of the key protected wild plant, namely the tutcheria hexalocularia in Guangxi can be broken through; protection of the rare plant tutcheria hexalocularia resource, and application and promotion thereof in landscaping are facilitated.
Owner:GUANGXI FORESTRY RES INST

Active soil for purifying high concentration polluted air in tunnels or other local areas

The invention discloses an active soil for purifying high concentration polluted air in tunnels or other local areas. The active soil contains microorganisms used for decomposing harmful gas in tail gas and is characterized by also including topsoil, perlite, pumice, turf, muck and artificial zeolite, wherein the volume ratio of topsoil to perlite to pumice to turf to muck is 4:2:1:1:1. The soil formula can solve the contradiction between the growth and reproduction of microorganisms and the physicochemical property of soil and ensure the activity of microorganisms in soil. The proportioning of ingredients causes the soil to have good air permeability, and the polluted gas can be evenly distributed in soil, thus further ensuring the utilization rate of the polluted gas by microorganisms. With higher purification efficiency of the polluted gas, the active soil saves use cost and can eliminate or abate the pollution to the atmosphere by high concentration vehicle tail gas in tunnels to the maximum degree.
Owner:招商局生态环保科技有限公司

A method suitable for large-area planting of sedum with ore

ActiveCN109006220BSpeed up the fertility processEasy to controlPlant cultivationCultivating equipmentsPlastic mulchSedum
The invention relates to a method suitable for large-area planting of sedum plumbizincicola. The method comprises the following steps: rotary tillage is carried out before transplanting next year, field parcel ridging is carried out in late February, and field parcels are covered with mulch films after herbicide spraying operation; in early March, transplanting of the sedum plumbizincicola is carried out, and perforating and transplanting operation is carried out on the mulch films; in middle July, the sedum plumbizincicola is cut while stubbles that are 3 to 5 cm in length are left uncut, andthe stubbles can grow again; the cut sedum plumbizincicola is cut into 8~10 cm single-branched cuttings which are then immersed in an acetylsalicylic acid solution before subjected being cottage operation, a sunshade was set up above a planting area and harvest can be reaped in middle November. Via adoption of the planting method disclosed in the invention, survival rates of the sedum plumbizincicola can reach above 90%, a two season propagation coefficient can reach above 40 times, and weed removal rates are controlled to be more than 60%. The method is characterized by simple operation, small investment, good effects and the like; a problem of large area weed control in sedum plumbizincicola plantation can be solved, costs can be greatly lowered, and survival rates and the biomass of the sedum plumbizincicola can be remarkably increased.
Owner:YONKER ENVIRONMENTAL PROTECTION
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