37results about How to "Solve reproductive problems" patented technology

The invention relates to a weather-resistance nutritional lick block for promoting the reproduction of cows. The main manner of cow breeding in China still takes a decentralized mode as the main part, and a decentralized breeding system can not support the high-cost daily ration with overhead price, so that dairy cows or meat cows always have breeding disorder and other nutritional and metabolic diseases. Experiences and experiences abroad prove that the feed supplementing lick block is an economical and effective technical approach under the condition. At present, the manufacturing technology of the special feed form, namely the lick block, in China is not mature. Particularly, the weather-resistance of the lick block is poor, so that the lick block can split after being blown by wind and wetted by rain, and the hardness of the lick block is reduced greatly; in addition, on the aspect of formula technology, the influential formula for preventing the breeding disorder for the cows does not exist. Therefore, the application and the promotion of the lick block in the cow breeding industry in China are limited greatly. The invention provides a formula of the weather-resistance type lick block for promoting the reproduction of the cows, and a preparation process thereof. As for the lick block, the weather resistance of the lick block is limited and improved, the reproduction performance of the dairy cows and the meat cows under the decentralized mode is improved by utilizing the special formula, and parorexia and other nutritional and metabolic diseases can be prevented and treated.

The present invention relates to a water purifier for producing electrolyzed hydrogen-containing pure cold and hot water, which dissolves hydrogen gas generated by electrolysis into raw water to produce electrolyzed-reduced water. More particularly, the present invention relates to a sealing-type water purifier and a water-purifying method for producing electrolyzed hydrogen-containing pure cold and hot water and preventing contamination caused by microorganisms and foreign contaminants.

The invention relates to a method for rapidly propagating roots of subprostrate sophora by tissue culture. The method provided by the invention comprises the following steps of: (1) taking seeds of the roots of subprostrate sophora as explants to be disinfected; (2) putting the disinfected explants into an MS (Murashige and Skoog) basic culture medium to be induced and germinated to obtain sterile test-tube seedlings; (3) putting the sterile test-tube seedlings into an MS propagating culture medium to carry out a rapid propagating culture on the sterile test-tube seedlings to obtain cluster buds; (4) putting the cluster buds into an MS sound seedling culture medium to carry out a sound seedling culture to obtain strong plants; (5) putting the strong plants into an MS rooting culture medium to be cultured to obtain complete seedlings with roots; and (6) taking the complete seedlings with roots to adopt seedling adaptation and then transplanting the seedlings to a sand bed to grow for one month; and then transplanting the seedlings to a land for growing field crops. The germchit obtained by the method is strong, has a high survival rate, and can supply a lot of good-quality germchits of the roots of subprostrate sophora in short order; and the method effectively solves the problem of a large-scale seedling culture of the roots of subprostrate sophora.

The invention discloses a wheat fertility-restoring gene and an application thereof, belongs to the technical field of plant biology and particularly relates to a recessive nuclear male sterility gene and cloning of a promoter thereof, and an application of the recessive nuclear male sterility gene in cross breeding. A fertility-restoring gene FRG1 is successfully cloned through a flow cytometry and a high-throughput sequencing method. The gene is capable of completely restoring the male fertility of a Lanzhou nuclear male sterility mutant or an allelic mutant thereof and a basis is laid for construction of a novel wheat hybrid breeding technology system. Meanwhile, more possibilities are provided for solving the technical bottleneck problems that an existing wheat two-line and three-line hybrid technology is unstable in sterile line fertility, limited in hybrid variety resource, complicated in seed production technology and high in seed production cost. The provided gene and a sterile line propagation and retention method have important significance and application value in wheat cross breeding work.

The invention discloses a wheat fertility restoration gene and its application, belongs to the field of plant biotechnology, and in particular relates to the cloning of a recessive nuclear male sterility gene and its promoter, and its application in hybrid breeding. The present invention successfully cloned a fertility restoration gene by means of flow cytometry and high-throughput sequencing FRG1 , the gene can completely restore the male fertility of the Lanzhou nuclear male sterile mutant or its allelic mutant, which lays the foundation for the construction of a new wheat hybrid breeding technology system. At the same time, in order to solve the technical bottlenecks of the existing "three-line" and two-line" hybridization technologies of wheat, such as unstable sterile line fertility, limited hybrid variety resources, complicated seed production technology, and high seed production costs, it provides more There are many possibilities. The gene and the breeding and maintenance method of the sterile line provided by the present invention have important significance and application value to the work of cross breeding of wheat.

The invention relates to a method for the propagation by cuttings and culture of seedling of Guangxi wild downy grape, and the propagation by cuttings is adopted, perlite, peat and sand are mixed uniformly according the volume ratio of 1:1:1 to be used as the substrate, wherein, the relative humidity of the substrate is 60 to 80 percent and the thickness of the substrate laid on an electric blanket is 10 to 15cm and a plastic film is covered on the substrate; the length of the cutting of the wild downy grape is 15 to 20cm, the base of the cutting is treated with a root-inducing regulator, thepart of the cutting exposed to the air is wrapped up by the plastic film to be inserted on the substrate; the temperature of the substrate is controlled at 30 DEG C at night, and 20 DEG C in the daytime. The technique of the invention has the prominent advantages of low cost, simple operation and easy promotion, with the cutting survival rate of more than 70 percent.

The invention discloses a grafting method of albizia falcataria. The grafting method includes the steps of cion cultivation, cion preparation, tree stock treatment, grafting and management after grafting. The grafting method of the albizia falcataria is easy to operate, the rate of survival of the grafted albizia falcataria is promoted to 70.4% by utilization of the method and is obviously improved compared with the rate of survival by utilization of a traditional method. The propagation problem of needed materials for building a garden in the process of building the clone seed garden of the albizia falcataria can be effectively solved, the grafting method of the albizia falcataria facilitates that the high yield and high quality albiazia falcataria is early grown in an improved seedling base, and lays the foundation for production and popularization of albiazia falcataria improved seedlings.

The invention relates to a killing method of bacillus subtilis, in particular to an application of freshly squeezed bayberry juice to bacillus subtilis killing. The application comprises the following steps: first, adding freshly squeezed bayberry juice in a liquid containing bacillus subtilis, and carrying out pressure maintaining treatment for 1-1.5 minutes under the high pressure higher than or equal to 10 MPa after uniformly mixing, wherein the freshly squeezed bayberry juice is at least twice of the volume dose of the liquid containing bacillus subtilis. Through the adoption of the application, the pressure requirement of high-pressure bacteria killing condition can be reduced greatly, and the continuous propagation during the microorganism storage period after bacteria killing is avoided.

The invention discloses a method for cutting and raising seedlings of tutcheria hexalocularia employing a container. An offspring plant container seedling which is kept consistent with an excellent single strain in inheritable characters is obtained in manners of selecting an excellent single strain of the tutcheria hexalocularia as a stock plant by an inventor; selecting a nursery land, and preparing soil; preparing and sterilizing matrix; collecting, cutting and processing cuttings; moisturizing, shading, managing after rooting, and the like. Blooming is earlier than sowing seedling, and the cutting survival rate and the transplanting survival rate are high. The method disclosed by the invention is simple to operate, low in production cost and applicable to vegetative propagation of excellent single strain of the tutcheria hexalocularia; the difficulties of propagating and transplanting of the excellent single strain of the tutcheria hexalocularia can be effectively solved; the method can be applied to massive cultivation of seedlings of rare plants and excellent ornamental tree tutcheria hexalocularia; the difficulty of seedling of the key protected wild plant, namely the tutcheria hexalocularia in Guangxi can be broken through; protection of the rare plant tutcheria hexalocularia resource, and application and promotion thereof in landscaping are facilitated.

The invention discloses a method for synergistically killing staphylococcus aureus through citruse naringenin and a high-strength pulsed electric field. The method comprises the following steps of adding naringenin to a liquid food containing staphylococcus aureus, to be sterilized, and performing pretreatment; and preforming sterilization on materials pretreated with the naringenin by the high-strength pulsed electric field. Preferably, the addition concentration of the naringenin is 12.5-125mg / L, the pretreatment time is 0.5-1.0h, the parameters of the pulsed electric field are set as that the electric field strength is 20-50kV / cm, the pulse width is 10-40[mu]s, the pulse frequency is 0.1-1.0kHz, and the total time that materials are subjected to pulsing treatment is 60-1000[mu]s. According to the method disclosed by the invention, a natural antibacterial ingredient namely the naringenin is combined with a high-strength pulsed electric field sterilization technique, so that the effect of killing the staphylococcus aureus is notably increased, the food is safe, the storage period is prolonged, and the loss of original flavor and original nutrient substances is reduced.

The invention relates to a method suitable for large-area planting of sedum plumbizincicola. The method comprises the following steps: rotary tillage is carried out before transplanting next year, field parcel ridging is carried out in late February, and field parcels are covered with mulch films after herbicide spraying operation; in early March, transplanting of the sedum plumbizincicola is carried out, and perforating and transplanting operation is carried out on the mulch films; in middle July, the sedum plumbizincicola is cut while stubbles that are 3 to 5 cm in length are left uncut, andthe stubbles can grow again; the cut sedum plumbizincicola is cut into 8~10 cm single-branched cuttings which are then immersed in an acetylsalicylic acid solution before subjected being cottage operation, a sunshade was set up above a planting area and harvest can be reaped in middle November. Via adoption of the planting method disclosed in the invention, survival rates of the sedum plumbizincicola can reach above 90%, a two season propagation coefficient can reach above 40 times, and weed removal rates are controlled to be more than 60%. The method is characterized by simple operation, small investment, good effects and the like; a problem of large area weed control in sedum plumbizincicola plantation can be solved, costs can be greatly lowered, and survival rates and the biomass of the sedum plumbizincicola can be remarkably increased.

The invention discloses a method for killing escherichia coli and especially discloses a use of a freshly squeezed red bayberry juice in killing of escherichia coli. According to the invention, a freshly squeezed red bayberry juice is added into a liquid containing escherichia coli, and the mixture is uniformly mixed and then is subjected to pressure maintaining treatment under the pressure greater than or equal to 10MPa for 1-1.5min, wherein the volume of the freshly squeezed red bayberry juice is at least 1 time volume of the liquid containing escherichia coli. The method provided by the invention can greatly reduce pressure requirements under high-pressure sterilization conditions and avoid microbe follow-up breeding in a storage period after sterilization.