Application of freshly squeezed bayberry juice to bacillus subtilis killing
A technology of Bacillus subtilis and freshly squeezed bayberry juice is applied in the field of sterilization method of Bacillus subtilis, and can solve the problems of high pressure level requirements, high pressure, and increased equipment cost investment in ultra-high pressure equipment.
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[0015] Activation of Bacillus subtilis (H110) (activation is a conventional method, for example, according to the already published "Bactericidal Effect of Ultrasonic on Bacillus subtilis in Liquid Milk" (China Dairy Industry, 2010 Issue 02) or "Manufacturing and Application" (China Agricultural Press, Chen Tianshou, 1995) or China General Microorganism Culture Collection Management Center (CGMCC) Microorganism Use Guidebook) after inoculation into pure water, so that the colony concentration of the bacterial liquid in the pure water reaches 10 7 More than cfu / ml.
[0016] Bacillus subtilis (H110) is a conventional strain, for example, it can be purchased from China General Microorganism Culture Collection Center (No.: ATCC 6051).
[0017] Squeeze fresh red bayberry juice and get the supernatant after centrifuging at 10000 rpm for 20 minutes. The supernatant is fresh red bayberry juice. The volume ratios of 1:1, 10:1, and 100:1 were added to the above bacteria solution respe...
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