88 results about "Caudal Vein" patented technology

The invention discloses a gold nanorod multifunctional probe-based nuclide-cerenkov luminescence-CT multi-mode imaging method, which applies a <68>Ga-AuNRs-RGD multifunctional molecular probe to the field of multi-mode molecular imaging. The caudal vein of a tumor-bearing mouse is injected with the <68>Ga-AuNRs-RGD multifunctional molecular probe, nuclide-CT imaging is performed by a PET / CT imaging system, cerenkov luminescence-CT imaging is performed by a CLI / CT (cerenkov luminescence imaging / computed tomography) system, and a PET / CT image serving as a source image and a CLI / CT image serving as a target image are fused by means of grey rectification to obtain a nuclide-cerenkov luminescence-CT multi-mode imaging display image based on the <68>Ga-AuNRs-RGD multifunctional molecular probe. According to the method, the <68>Ga-AuNRs-RGD multifunctional molecular probe is applied to multi-mode molecular imaging, has the advantages of increasing optical signal intensity, improving imaging resolution, improving imaging depth and the like, and has the characteristics of excellent dispersion, low toxicity, high living cell membrane permeability and the like.

The invention discloses a stem cell preparation for treating cirrhosis, which is prepared from stem cells from human umbilical cord placenta, normal saline containing seralbumin with concentration by volume being 0.5-5%, and low-molecular-weight heparin sodium with concentration being 50-701U / ml. When the stem cell preparation is transfused into a rat model with cirrhosis through a caudal vein, the effective dose of the stem cell preparation is 5*106 cells each time; and the effective dose of the stem cell preparation is 0.5-5*106 cells / kg each time when the stem cell preparation is transfused into a patient with cirrhosis through a peripheral vein. After applied to rats with cirrhosis and patients with cirrhosis, the stem cell preparation can obviously improve the survival rate of the rats with cirrhosis and the patients with cirrhosis, retard and invert a cirrhosis process, improve a whole state and a liver function state, and promote pathological recovery of liver, thereby developing a new way to cell therapy for treating cirrhosis.

The invention belongs to the technical field of animal models, and particularly relates to a method for establishing a PDX model of high leukocyte leukemia, and the method comprises: screening a suitable clinical patient with the high leukocyte leukemia; using a COM. TEC blood cell separator to separate leukocytes from peripheral blood; separating and purifying mononuclear cells of the leukocytescollected from the peripheral blood for cell culturing to obtain leukemia cell suspension; preparing the PDX model of high leukocyte leukemia, to be more specific, firstly using X-ray for irradiationof mice, injecting the leukemia cell suspension to caudal veins of the mice, one week later, drawing blood of tails of the mice randomly for detection, and judging whether the model is successfully established according to detection results. The method for establishing the PDX model of the high leukocyte leukemia has a high success rate of modeling, disease manifestation and cell morphology of theconstructed PDX model of the mice can directly reflect the characteristics of high leukocyte leukemia cells.

The invention discloses a caudal vein joint intraperitoneal injectionfixing device which comprises a base, a cylinder, a front cover and a rear cover; the cylinder comprises a side plate and a slidingrail, a first sliding groove is formed in the side plate, the sliding rail and the side plate form an annular structure with the adjustable size, a track is connected to the side face of the front cover, a sliding hole is formed in the side plate, the track is slidably arranged inside the sliding hole, a locking mechanism is arranged at the side plate, the locking mechanism comprises a clamping base, a first clamping plate, a second clamping plate, a first spring and a first baffle, a second sliding groove is formed in the clamping base, the second clamping plate is slidably arranged inside the second sliding groove, the first clamping plate and the second clamping plate are arranged at the two sides of the track, the first baffle is hinged to the side plate, and the first baffle is provided with a circular bead and a limiting plate. A mouse is fixed so that an operator can perform the injection operation conveniently, force for clamping the mouse is fixed, and the phenomenon that blood circulation of the mouse is affected due to force changes or the mouse is killed in the testing process is avoided.

The invention discloses a separation culture method of the primary hepatocyte of a jian carp. The separation culture method comprises the following steps: selecting a healthy jian carp without injury, collecting blood from the caudal vein of the jian carp, then sterilizing the surface of a fish body, placing into a super clean bench, and aseptically collecting the liver of the jian carp; rinsing by using a PBS solution which contains double antibodies, adding a trypsin digestion solution, wherein the temperature of digestion treatment is 26-28 DEG C, and the time of the digestion treatment is 15-20 minutes; after digestion is finished, adding to a culture medium A to finish the digestion, filtering the trypsin digestion solution by using a filter screen of 200 meshes, and collecting filter liquor; and respectively centrifugalizing 50 grams of the filter liquor and 30 grams of the filter liquor for 5 minutes, then washing twice by using a culture medium B, removing a supernatant to obtain a precipitation, namely an extracted hepatocyte, preparing a cell suspension, and planking for culture. The experimental method disclosed by the invention is simple and fast and can save the separation time to a great extent. The separation time adopted by an experiment is about 40 minutes, and the obtained hepatocyte has the advantages of good growth condition, small erythrocyte quantity and cell survival rate of about 95%.

The invention relates to the technical field of biology, in particular to a leukemia mouse model based on a gene co-transfection technology and a preparation method thereof. The preparation method of the leukemia mouse model comprises the following steps: performing construction and packaging of K-ras mutants and AML1-ETO fusion gene lentivirus vectors; performing bone marrow cell separation and virus infection condition monitoring; implanting infection cells in a mouse to build a leukemia animal model; and performing model identification. The method of building the leukemia mouse model in a mode of utilizing caudal vein injection to lead in the manual site-directed mutagenesis K-ras mutants and AML1-ETO fusion gene lentivirus vectors is adopted initiatively. The leukemia mouse model is high in success rate, pathological characteristics are high in similarity to morbidity conditions of clinical leukemia, and the novel animal model can be provided for leukemia extramedullary infiltration mechanism research, leukemia medicine screening and gene and molecule target treatment.

The invention discloses a mouse caudal vein injection fixing device and an application method thereof. The mouse caudal vein injection fixing device comprises a base, a connecting plate, a fixed cylinder and a telescopic rod, wherein a first bearing seat and a second bearing seat are arranged on the base; the first bearing seat is hinged with the lower end of the connecting plate; a mounting plateis hinged at the upper end of the connecting plate; the fixed cylinder is hinged with a third bearing seat located on the mounting plate through a connecting piece; a fourth bearing seat is arrangedon the lower end surface of the mounting plate; two ends of the telescopic rod are hinged with the fourth bearing seat and the second bearing seat respectively; a tail blocking piece with a notch is hinged with an opening of the cylinder body of the fixed cylinder; a head blocking piece is arranged in the cylinder cavity of the cylinder body of the fixed cylinder and driven by a driving unit to slide; an annular air bag is arranged in the cylinder cavity of the cylinder body of the fixed cylinder and communicates with a pressure adjusting unit; and a tail placing block is slidably connected with and arranged in the sliding groove of the mounting plate. The objective of the invention is to provide the mouse caudal vein injection fixing device which is more convenient to operate, more flexible to adjust and better in adaptability to the body type of a mouse.

The invention discloses an injecting and fixing device for a mouse. The device comprises a shell, wherein a fixing board is arranged in the shell, a mouse tail placement table is connected to the lower end of the shell through a connecting rod a, a rotating head is arranged on the mouse tail placement table, a connecting rod b is connected to one end of the rotating head, and an injecting device is connected to the other end of the rotating head. For the injecting and fixing device for the mouse, the fixing board is made of a soft material, the animal is fixed through the intrinsic joint bending of the animal, and adjustment can be carried out according to the body size of the animal; a sodium lamp is placed in the mouse tail placement table, so that the development for the caudal vein blood vessel can be realized, the heat generated by the sodium lamp can heat the mouse tail, so that the double effects including expanding the caudal vein blood vessel can be achieved; the injecting device is rotated through the connecting rod b with angle scales, the rotating head and a rotating rod, and the accurate positioning for an injection syringe is realized; the device provided by the invention is compact and portable in structure, is simple and easy to use, is good in repeatability, can realize injection through multiple ways, and has low requirement on the technology of experiment personnel.

The invention belongs to medical evaluation and detection technology field. It concerns in particular to the construction method of animal model for skin allergy therapeutic drug screen and skin allergy mechanism study. The skin allergic mouse model is built by injecting anti DNP IgE monoclonal antibody to BALB / c mouse caudal vein and provoking the action by making 2,4 dinitroflurobenzene as antigen. The model can imitate the skin allergy dynamic reaction whole process favorably, therefore, it can be used for the screening of skin allergy treating drug and study of skin allergy pathogenesis and drug action mechanism.

The invention relates to a method for preparing red cell outer membrane protein of left- -eyed flounders, Paralichthys olivaceus, comprising the following steps: blood is taken from the caudal vein of the left-eyed flounder; the Alsever gelation inhibitor and the peripheral blood of the left-eyed flounder are mixed; the blood is diluted by PBS buffer solution, mixed with separating medium and centrifugated at the temperature of 4 DEG C, and the deposit formed by the red cells at the bottom is centrifugally washed at the temperature of 4 DEG C by the PBS buffer solution to remove supernatant fluid; hypotonic buffer solution is added and kept at the temperature of 4 DEG C to fully swell the red cell so that the membrane is ruptured; the deposit of the red cell membrane of the left-eyed flounder is centrifugally collected at the temperature of 4 DEG C; the hypotonic buffer solution is added and then the deposit of the red cell membrane is scattered by a whirlpool mixer and centrifugally washed at the temperature of 4 DEG C; membrane protein lysis solution is added and stirred to fully cleave the red cell membrane of the left-eyed flounder; after centrifugating at the temperature of 4 DEG C, the supernate is the extracting solution of the red cell membrane protein of the left-eyed flounder; after being subpackaged, the extraction solution is preserved in an refrigerator at the temperature of minus 80 DEG C. Compared with the prior art, the method is simpler and more efficient, thereby not only ensuring the high efficiency of the preparation of membrane protein, but also maintaining the biological activity of the membrane protein.

The invention discloses a rat fixator which solves the problems that the conventional device cannot fix a rat well, an instrument is complex and the cost is high. The rat fixator comprises a head fixing piece, a tail fixing piece and an anesthesia piece, wherein the tail fixing piece is connected with the bottom end of the head fixing piece; the anesthesia piece is connected with the top end of the head fixing piece; the end part of the top end of the tail fixing piece is provided with more than two cracks; the cross section of the head fixing piece is gradually enlarged from the top end to the bottom end; the cross section of the tail fixing piece is gradually shrunk from the top end to the bottom end; the head fixing piece and the tail fixing piece are of cavity structures; and the top ends and the tail ends of the head fixing piece and the tail fixing piece are of opening structures. The rat fixator is reasonable in design, simple in structure and firm in fixation, does not injure the rat and particularly facilitates batch production; and the rat fixator can conveniently perform test operation such as caudal vein blood collection, injection, tail flick test, pressing pain measurement and the like.

The invention belongs to the field of natural medicinal chemistry, in particular to separation and extraction of natural anti-inflammatory drug molecules and an application thereof. The method comprises the following steps of extracting by acidic ethanol, decompressing and concentrating, adding water for dissolving, loading a sample in XAD-7 macroporous resin, eluting, collecting and concentrating, freeze-drying to extract the mangosteen pericarp anthocyanin. Experimental results show that mouse peritoneal fluid neutrophil exudation can be effectively inhibited after the mangosteen pericarp anthocyanin is injected into caudal vein of the mouse, the inhibition ratios are respectively 34%, 77% and 94% while the dosages are respectively 0.01, 0.1 and 1mg / ml. A laminar experiment using an in-vitro simulated capillary proves that the adhesion inhibition ratios of the mangosteen pericarp anthocyanin on neutrophil are respectively 45%, 71% and 89% while the dosages are respectively 0.01, 0.1 and 1mg / ml. Therefore, the anti-inflammatory activity of the mangosteen pericarp anthocyanin is proved, and the mangosteen pericarp anthocyanin as a novel anti-inflammatory drug has good application prospect.

The invention discloses an establishment method and application of a novel rat hypertension model. The disclosed establishment method of the novel rat hypertension model includes the following steps that 1, a human-body intron-derived 27nt-microRNA sequence is provided to construct its high expression plasmids; 2, the high expression plasmids in the step 1 is mixed with an X-treme GENEHP DNA transfection reagent in a certain proportion, after dilution with normal saline, the reagent is injected instantaneously in a SD rat at high speed from the caudal vein of the rat, the blood pressure of therat is increased to a high level, and the novel rat hypertension model is formed. The rat hypertension model is an effective animal disease model, can be used for verifying the influence of human-body intron-derived 27nt-MicroRNA on the blood pressure and vasodilator factors of the normal rat and screening antihypertensive drugs, and has wide application prospects.

The research group selects the optimum method for preparing a product of the invention, namely, enabling 100nM miR-21inhibitor transfected by lipo 2000 RNAimax to enter the stem cell. The stem cell product prepared by the method is enhanced in the capacity of secreting transforming growth factor TPF-beta 1, thus the capacity of inducing the T cell to differentiate towards regulatory T cells after co-culture of the cell and the T cell is enhanced, namely, the immunoregulation capacity of the stem cell is enhanced. In an in-vivo experiment, a medicine is adopted for inducing a mouse for establishing an acute enteritis model, the stem cell and a control cell which are transfected by miR-21 inhibitor are transfused into the body of the mouse through caudal vein by using the dosage of 1X10<6>, the result shows that the clinical symptoms and the immune indices of the mouse with stem cell group transfected by miR-21 inhibitor transfused are superior to those of the control cell group with transfusion, namely, the stem cell transfected by miR-21 inhibitor can be used for more effectively treating mouse enteritis. All in all, the stem cell with strong and stable effect is prepared, and experimental basis is provided for improving the application efficiency of the stem cell in clinic.

The invention discloses a caudal vein hemostix and a blood collection method thereof, which belong to the field of manufacturing agriculture machinery. The hemostix is composed of three parts of a tail head fixing part, a caudal vein location part and a blood collection part. When the caudal vein hemostix is used, the caudal vein hemostix is fixed at the position of a tail head of an animal, a caudal vein is located through a cylinder, then a blood collection needle is inserted into the caudal vein through a blood collection needle opening to be fixed, and the blood collection needle and a vacuum blood collection pipe begin to collect blood. The caudal vein hemostix is simple, practical, time-saving, labor-saving, safe and suitable for quarantine of large animals of oxen, horses and the like or blood collection of scientific researches.

The invention discloses a separated culture method for primary hepatocyte of megalobrama amblycephala. The method comprises the following steps: selecting healthy megalobrama amblycephala, the weightof which is 30-50 g; fully disinfecting the megalobrama amblycephala with 1% potassium hypermanganate; sampling blood from a caudal vein; carrying out sterile dissection to take liver; digesting the liver with collagenase and filtering the liver with a 200-mesh cell screen to obtain a cell suspension; removing residual erythrocytes in liver cells by an erythrocuyte lysate; then carrying out gradient centrifugation to remove cell fragments; adding a proper amount of full culture medium suspension cells into the obtained cell precipitate; then calculating and adjusting the cell concentration with a cell counter; after paving a board, putting the board in a 5% CO2 cell incubator to be cultured at 28 DEG C; observing the adherence condition within 48 hours. The method provided by the inventionseparates and cultures liver cells by combining the characteristics of a researched species creatively, and the survival rate of the obtained cells reaches 90% or higher which meets the primary culture demand, thereby providing a theoretical foundation and a technical support for further carrying out related experiments of primary hepatocyte of megalobrama amblycephala.

The invention discloses a multifunctional experimental mouse operating device, comprising a cuboid-shaped box and a top cover, wherein one end surface of the cuboid-shaped box is an opening surface, and the other end surface is a blind end and is provided with a round tube communicating the inside and outside of the box; the bottom surface of the box, near the opening surface, is provided with a notch; the top surface of the box is semi-closed; and the top cover is movably connected with the box and can open or close the box. According to a multifunctional experimental mouse operating method disclosed by the invention, the multifunctional experimental mouse operating device is used, intraperitoneal injection, intraperitoneal anesthesia, atomizing operation, caudal vein blood sampling and tail fixation of an experimental mouse can be finished in the box in a closed state, the fixation and trachea medication of the experimental mouse can be finished in the box in an open state, and every operation can be finished by one person, so that the operation is simple, the experimental process is greatly simplified, and manpower is saved.

The invention provides a method for constituting presenile dementia bandicoot model by injecting Hcy to caudal vein, abdomen or ventriculus laterali cerebri of the bandicoot, which can induce the brain mantle and hippocampal cortex amyloid of the bandicoot to deposit to generate age pigment and lead tau undertake exceeding abnormal phosphorylation as well as appear compound symptom like academic handicap and dysmnesia, etc. The model can be used for defending presenile dementia, screening new medicines, appraising the curing effect and researching the nosogenesis.

The invention discloses a doped self-assembled nano fiber structure and a preparation method thereof. Through synergistic interaction of molecules, nano fiber can obviously change near-infrared absorption of a cyanine dye, thereby obviously improving photo-acoustic performance and photo-thermal performance. Phosphatase response synchronous-assembly processes and diagnosis and treatment performance of assembly materials are verified respectively through cell and in vivo experiments. Particularly, through caudal vein injection, after 4 hours, tumors and peripheral normal tissues can be very clearly distinguished. According to the invention, the cyanine dye-doped nano fiber with in situ formation of tumor tissues can be used for fluorescence, photo-acoustic imaging and photo-thermal therapy.

The invention relates to Tabanusyao Macquart antithrombotic enzyme tablysin and a gene and application thereof, and belongs to the field of biomedicine. In the invention, a natural antithrombotic enzyme is separated by a biomechanical method and a coded gene of the enzyme is amplified; and a gene engineering product which has equivalent activity with natural protein is obtained by using an escherichia coli expression system. The Tabanusyao Macquart protease tablysin is active protein which is separated from a Tabanusyao Macquart salivary gland, wherein the molecular weight is 27KDa; and an amino acid sequence of the enzyme is shown as SEQ ID NO. 1. The gene of the Tabanusyao Macquart protease tablysin consists of a nucleotide sequence which is shown as SEQ ID NO. 2. Through the TabanusyaoMacquart protease tablysin, fibrinogen can be hydrolyzed, platelet aggregation can be suppressed, and carrageenin-induced rat caudal vein thrombosis can be suppressed; and the Tabanusyao Macquart protease tablysin does not have bleeding activity and can be applied to preparation of medicaments for treating thrombotic diseases.

The invention relates to application of nicotinamide adenine dinucleotide as an active ingredient in preparation of a drug for preventing or treating heart ischemic injuries. In experiments, caudal vein normal saline injection and a heart ischemia operation are carried out on rats in a control group, it is found through research results that a great number of apoptosis signals (TUNEL positive signals) appear on the hearts, while apoptosis signals of rats, on which caudal vein NAD+solution injection is carried out, in an experiment group can be effectively reduced.

The invention discloses an application of a PDL1-IgGFc fusion protein in inhibition of severe malaria morbidity, and belongs to the technical field of anti-malaria drug preparation. A fusion gene of a PDL1 molecule extracellular fragment and an IgG molecule Fc fragment is constructed, the fusion gene is constructed into adenovirus or is expressed in vitro, and the fusion protein is verified to be successfully expressed by a Western Blot method. In in-vitro cell experiments, the fusion protein can significantly inhibit ConA induced CD8+T cell activation; at the same time, in mice in-vivo cell experiments, through caudal vein injection of recombinant adenovirus for expressing the PDL1-IgGFc fusion protein, occurrence of cerebral malaria caused by infection of a plasmodium berghei ANKA strain can be significantly alleviated, and the survival time of mice is prolonged. The PDL1-IgGFc fusion protein is indicated to have a role in inhibiting severe malaria morbidity, and the inhibition role is involved with inhibition of CD8+T cell activation. The fusion protein provides a new drug selection for treatment of cerebral malaria and other severe malaria in clinic.

The invention discloses an electrically conductive macromolecular nano-material and an application of the electrically conductive macromolecular nano-material. According to the electrically conductive macromolecular nano-material, poly (3, 4-ethylidene dioxythiophene) / poly styrene sulfonic acid is taken as a substrate, wherein nano-particles formed by modifying polyacrylamide hydrochloride, polyacrylic acid and polyethylene glycol on the poly (3, 4-ethylidene dioxythiophene) / poly styrene sulfonic acid layer by layer. The nano-material is further connected with some fluorescent dyes or chemical drugs. The electrically conductive macromolecular nano-material can prepare photo-thermal therapeutic agent for treating cancer. The electrically conductive macromolecular nano-material has very strong optical absorption property in near-infrared area, forms a superbly high enrichment on the tumour position in the way of caudal vein injection and has very good treating effect under exposure to laser.

The invention relates to a mouse model with lung metastasis of gastric cancer and an establishment method thereof, and belongs to the technical field of experimental animals. According to the mouse model and the establishment method thereof, BGC-823 and MKN-45 gastric cancer cells are cultured in vitro, and resuspended to a proper concentration with sterile PBS when the cell viability is strong. After the cells are cultured inside the body of immunodeficient NUDE and SCID mice inoculated in immunodeficience through the caudal vein, the mouse model with lung metastasis of gastric cancer is successfully obtained by the identifications including the statistics of surface lung nodules, HE staining and IHC staining. According to the mouse model establishment, needed experimental conditions and operation are simple and convenient, lung metastasis lesions after the experiment are obvious, metastasis efficiency is high, and mice with metastatic lesions have moderate survival cycles. In the evaluation and statistics of the metastasis results, the macroscopic counting of the lung nodules is improved into the microscopic counting of the IHC staining positive cells, thereby obtaining more objective and accurate results. The animal model of tumor metastasis may be widely used in scientific research and teaching.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

The invention relates to a melanin / Ce6 photodynamic nano tumor drug capable of improving the light absorption as well as preparation and application of the melanin / Ce6 photodynamic nano tumor drug. The nano tumor drug is prepared by virtue of the following method: preparing Ce6 into a DMSO mother solution, dropwise adding the DMSO mother solution into a melanin aqueous solution, mixing, dialyzing,thus obtaining the melanin / Ce6 photodynamic nano tumor drug, wherein a mass ratio of a melanin nano particle carrier to photodynamic drug Ce6 is 1 to (0.01 to 10). After the melanin / Ce6 photodynamicnano tumor drug is injected into a nude mouse body via caudal vein, the enrichment effect on a tumor part is good, in the photodynamic treatment process, the melanin can absorb the energy of the light, the temperature of tissues on a treated part is increased, the photodynamic treatment effect of a photo-sensitizer is improved, and the specific photodynamic treatment effect on local tumor is good;and in addition, the melanin / Ce6 nanometer can further wrap and carry other chemotherapy drugs, photodynamic drugs, photoacoustic probes, nuclear magnetism probes, genes, polypeptides, protein targeting molecules and the like, so that the application prospect is wide.

The invention discloses an application of carbon monoxide molecules in inhibition on acute rejection after skin grafting. According to a large number of cell tests, the inventor discovers that after the carbon monoxide molecules are dissolved into a dimethylsulfoxide (DMSO) solvent and placed into an organism, CO can be continuously released, so the carbon monoxide molecules can serve as exogenous CO donors. The skin of ICR mice and BALB / C mice can serve as a donor and a receptor; an allogeneic gene acute rejection model can be established; exogenous CO is injected into the caudal veins of the mice; and early intervention and the test are performed, so that the carbon monoxide release molecules have inhibition effect on the acute rejection after skin grafting. The carbon monoxide release molecules are used for inhibiting the acute rejection after skin grafting; the concentration is controlled relatively easily and correctly; during long-term use, the carbon monoxide release molecules are added conveniently; and the carbon monoxide molecules can serve as an immuno-inhibitor with high specificity, low toxicity and high efficiency.

The invention discloses a construction method of a chronic hepatitis E virus mouse model. In the method, HEV strains are derived from feces of chronically infected rhesus monkeys, and the rhesus monkeys continuously expel toxin for 93 weeks; after an HEV virus suspension is inoculated into BALB / c mice through caudal veins, HEV RNA can be continuously detected in feces and serum of the mice, and the HEV RNA has high virus copying property, and the continuous positive time can reach 36 weeks. The method for establishing the model is simple, can be used for researching the persistent infection mechanism of HEV, and can provide an important animal model for screening and evaluation of anti-HEV drugs.

The invention discloses an experimental mouse caudal vein micro injector, which comprises a syringe tube, a push rod, an electric pushing block, an electronic pump, a syringe needle base, a soft hose and a syringe needle, wherein the push rod is arranged in the syringe tube in a movable mode; and a micro flow meter is arranged on the electronic pump. The back end of the micro flow meter is connected to the syringe tube; the front end of the micro flow meter is provided with the flexible soft hose; the front end of the flexible soft hose is connected to the syringe needle base; the needle head is detachably arranged at the front end of the syringe needle base; and the syringe tube is detachably arranged on the electronic pump. A motor and a toothed strip are arranged on the electronic pump; the motor is meshed with the toothed strip by virtue of a gear; the electronic pushing block is arranged on the toothed strip; a sliding guide rail is arranged on the electronic pump; a display screen, a timer and a pushing block sliding cavity are arranged on the electronic pump; the display screen and the micro flow meter are connected; the timer and the motor are connected; and flow regulating buttons are arranged on the display screen. With the implementation of the micro injector disclosed by the invention, it can precisely control an injection speed and conduct metering, which is conducive to the accuracy of experimental results; the syringe needle does not easily become fallen and blood vessels are not easily damaged;and caudal vein injection can be completed by one person; therefore, the micro injector is labor-saving and is convenient to operate.

The invention discloses a method for carrying out anesthesia and blood drawing on Takifugu obscurus, which comprises the following operating steps of: firstly, adding pood water into a container; regulating the water temperature into 15 to 20 DEG C; adding clove oil into the container to prepare anesthetic solution with the weight ratio of the water to the clove oil of 99.98:0.02; placing the Takifugu obscurus into the anesthetic solution, wherein the anesthesia time is 2 to 5 minutes; drawing blood by a syringe at the caudal vein, wherein the blood drawing time is 1 to 1.2 minutes; and after drawing blood, transferring the Takifugu obscurus into a temporary culturing pood for revival. According to the invention, aiming at the physiological property of the Takifugu obscurus, the method is successfully used for carrying out anesthesia and blood drawing on the Takifugu obscurus, has low cost, is simple and convenient to operate, has small damage to the fish body and has a large blood drawing quantity.