159 results about "Animal brain" patented technology

The invention discloses an implantable nerve micro-stimulation and collection remote control chip. The invention includes a brain nerve electrical stimulation and collection module, a nerve micro-stimulation and collection electrode array, an uplink wireless radio frequency communication module and a downlink wireless radio frequency communication and an energy coupling power supply module; the whole remote control device is arranged in the animal brain; the downlink wireless radio frequency communication and energy coupling power supply module receives the command which is send in from the outside, the brain nerve electrical stimulation and collection module is controlled to generate the stimulation signal and is responsible for the energy supply of the whole implantable chip remote control device by utilizing the radio frequency energy coupling principle after the analysis; the uplink wireless radio frequency communication module carries out the wireless output of the nerve electrical signal which is sent in by the brain nerve electrical stimulation and collection module; by implanting the chip in the animal brain, the invention can lead to a more compact system for collecting and controlling the physiological signals of animals, reduce the influence of the outside devices on the animals and increase the concealment and quality safety of the future practical field.

The invention provides a neural stem cell (NSC) strain. The NSC strain can be isolated from animal brain tissues from different species. Embryonic stem cells (ES), induced pluripotent stem cells and other stem cells can be differentiated into the NSC. The NSC strain is characterized by quick proliferating and stable passaging; prolonged expressing biomarker proteins of Oct4 and Sox1; high efficient being differentiated into a plurality of neurons and glial cells. The invention further provides an establishing method and an application for the NSC.

Phosphatidylserine is extracted from animal brains by: drying animal brains, smashing them into granules, putting propanone into the granules, stirring and extracting, separating propanone supernatant liquid contained cholesterine and oils from the brain granules, steaming the residues to be dried, adding n-hexane or alcohol or ammonia water alcohol into steamed dry residues, stirring and extracting, separating extracting liquid contained phospholipid compositions, steaming the residues again, putting ether into steamed dry residues, stirring, freezing and defreezing, filtering to remove deposits, removing the remained phospholipid further, adjusting supernatant liquid pH=3-6, steaming to remove ether, and steaming to dry crude phosphatidylserine again, dissolving crude phosphatidylserine dry powders in n-hexane, adding sodium acetate alcohol liquid, stirring, settling to be laminated, removing the supernatant contained inositol phospholipid, finally, steaming to dry the n-hexane in supernatant liquid to obtain high pure phosphatidylserine under reduced pressure.

The invention provides a preparation method for monosialotetrahexosyl ganglioside. The method comprises the following steps of: 1) weighing animal brain tissues, stirring and extracting by taking aqueous solution of a detergent as an extraction solvent and removing precipitate to obtain extract; and 2) concentrating the extract to obtain monosialotetrahexosyl ganglioside crude extract. The invention also provides a preparation comprising monosialotetrahexosyl ganglioside sodium. The preparation has the advantages of no use of organic solvent during the extraction of the monosialotetrahexosyl ganglioside, simple preparation process, suitability for batch production, environmental friendliness and capability of reducing the production cost and the production period. The invention also provides a monosialotetrahexosyl ganglioside injection prepared by the method; and the injection has high purity, does not contain an organic solvent and can improve the safety of clinical administration.

Disclosed are methods and compositions for an animal model of Parkinson's disease. In particular, disclosed is the use of antisense compounds to inhibit the expression of ALDH1A1 in the substantia nigra of an animal brain for the purpose of creating an animal that will displays the symptoms of a human with Parkinson's Disease, including various biochemical, histological, and behavioral characteristics. Also disclosed are methods for using the animal model for Parkinson's disease to test potential therapeutic agents for Parkinson's disease.

The disclosed drug detector based on animal olfaction comprises: a CCD camera, a brain stereotaxic instrument, an electrode, a pre-amplification and filter circuit, a multiarm maze, and computer control and analysis system. Wherein, according to animal brain olfaction cortex neuron electric action, letting animal produce specific memory to volatile drug; random putting drug in small rooms of the maze to give prize stimulation for specific brain area and reinforce the memory till animal action includes statistic meaning, collecting relative induced brain electric information to input computer for recognition and analysis and form training database.

The invention discloses a method for preparing a brain tissue frozen section, belonging to the fields of biology and medical experiments. The method comprises (1) a step of tissue extraction, immobilization and dehydration, namely a step of subjecting an animal brain tissue to immobilization and gradient dehydration; (2) a step of freezing pretreatment, namely a step of washing the brain tissue with a PBS solution, wiping the brain tissue, leveling the brain tissue, wrapping the brain tissue with tin foil paper, freezing the brain tissue in a rapid freezing zone of a freezing microtome, and then taking the tissue out for subsequent treatment; (3) a step of semi-embedding treatment, namely a step of subjecting the brain tissue to semi-embedded treatment, and allowing the tissue to stand for1 minute; (4) a step of frozen sectioning, namely a step of fixing a sample holder to a probe of the microtome, and performing continuous coronal frozen sectioning; and (5) a step of section collection, namely a step of collecting sections of desired positions into a six-pore plate containing a frozen preserving fluid, and storing the sections at -20 DEG C for long time. Semi-embedding treatmentis adopted to treat the brain tissue, and therefore, influences of an embedding agent on the brain tissue are avoided, and sections are complete and free of ice crystals, and have good immunofluorescent staining properties.

The invention discloses a method for preparing a whole brain specimen of a baby animal. The steps are as follows: a. a baby animal brain sample is fixed and imbued; b. the fixed and imbued brain sample is blackened by using blackening liquor that is alkali liquor with the pH value being more than 10, and the blackening period is 1-24 hours; c. the obtained brain sample is dehydrated, and the dehydration is carried out by 100% dehydrating agent in weight; d. the blackened brain sample is gummed by using an embedding agent, and two continuous gumming are carried out by 100% embedding agent in weight; e. after being gummed by the embedding agent, the brain sample is put into an embedding box and then submerged by adding the 100% embedding agent in weight; f. the embedding box for submerging the brain sample is heated, so that the embedding agent is polymerized, the polymerization temperature is 35 to 80 DEG C, and the polymerization period is 6-96 hours. The method for preparing the baby animal brain specimen of the invention can dye more neurons as well as neuron cell bodies, dendrite and axon; the contrast is high; the color is not faded easily; and whole brain neuron three dimensional morphology structures can be observed in the sub-micro scale.

An embodiment of the present invention relates to a method for visualizing at least one human or animal brain segment in order to aid a stimulation or manipulation of the brain, said method comprising the steps of:(a) predicting the localization of where a stimulation or manipulation effect is or would be, if and when initiated, and determining at least one target brain segment which is or would be stimulated or manipulated;(b) evaluating whether at least one brain segment is functionally correlated to said at least one target brain segment;(c) providing image data which visualize the at least one target brain segment and / or at least one of the correlated brain segments as evaluated in step (b); and(d) displaying the image data.

The invention relates to high purity gangliosides preparation method. It includes the following steps: using higher animal brain cell as raw material; adding solvent to homogenate and do de-lamination; processing gnagliosides extracting solution by once new type macroporous resin column to gain the gangliosides which purity is more than 98%. The known gangliosides compound has extensive biological activity. And its main function is to cure and improve impaired neurological disease. The invention adopts new type macroporous resin for the first time, utilize resin structure to remove harmful substance, separate effective constituent, and keep its biological activity. It is fit for industrialization production.

The invention belongs to the field of biotechnology and relates to a street virus glycoprotein derivative polypeptide-modified brain-targeted nanometer gene delivery system and a preparation method thereof. The preparation method comprises the following steps of: connecting a hydrophilic polymer with an RVG29 polypeptide to construct a brain-target carrier by adopting a street virus glycoprotein derivative peptide RVG29 as a brain-targeted head group and cationic dendritic polymers as carrier materials; and compounding the brain-target carrier and a plasmid deoxyribonucleic acid (DNA) for coding a specific gene through an electrostatic compound effect to prepare the brain-targeted nanometer gene delivery system. The intake and penetration efficiency of the nanometer gene delivery system on an in-vitro blood brain barrier model can be increased; the accumulation of the nanometer gene delivery system in an animal brain is increased; the expression quantity of a reporter gene in the brain is increased; the gene is delivered into the brain when crossing a blood brain barrier; and a high-efficiency safe strategy is provided for the application of diagnosing and treating gene in the brain.

The invention relates to a preparation method and application of monosialotetrahexosylganglioside. The method comprises the following step: by taking a cationic resin (preferably Dowex50) as a medium, in an acidic mixed water solution containing chloroform, translating a ganglioside mixture derived from an animal brain tissue into a mixture enriched with GM1. The GM1 content in the obtained translation product is increased from 7-10% before translation to 72-80%, so that the consumption of the animal brain tissue can be reduced by 7-12 times (for producing the same amount of GM1), thereby obviously lowering the production cost.

The invention discloses a creative method for commercially producing hydrolyzed brain protein powder and cephalin by animal brains. The method comprises the steps of using supercritical carbon dioxide to remove grease, performing enzymolysis and using active carbon to decolorize; using macroporous resin to adsorb impurities, flowing out brain protein hydrolysate; using cation-anion resin to desalt; using the thin film to condensate the product, performing spray drying; obtaining hydrolysated brain protein powder and purifying the paste in a separation kettle I. The method overcomes the problem that the method for removing grease by the organic solvent produces residual solvent to cause the toxic reaction, removes grease cleanly, is easy to hydrolyze and filter, and strengthens fishy smell-removing and decolorizing ability of the active carbon due to macroporous resin; the creativity is as follows: the obtained hydrolysated brain protein powder is pink-white, has no odor and toxicity, and has high cephalin; two products can be produced at the same time, which can be applied in nutritious foods, health care products and foods, and can be manufactured drugs after being purified further.

The present invention relates to a method for producing glycolipides substance using ganglioside as main component, sand method includes the following steps: pulverizing animal brain tissue, adding 20-100% alcohol solvent to make extraction, in which the ratio of dose of alcohol and weight of brain tissue is 1-10:1, filtering or centrifugalizing, concentrating filtrate, the concentrate and its freeze-dried powder can be diluted with water or mixed with edible auxiliary material, and can be made into the oral brain health-care product with various forms.

The present invention relates to a method for extracting and purifying ganglioside, which separates out neutral lipid impurity components from the preprocessed animal brain tissues by supercritical extraction under the function of supercritical fluid (SCF), entrainer is then added into the supercritical fluid (SCF) to unceasingly extract animal brain powder without neutral lipid impurities, and polar ganglioside components are selectively resolved to produce the ganglioside (GLS), which then is treated by purification process, such as adsorption chromatography separation or ion exchanger separation, etc., to produce high-purity ganglioside components. The operation of the method is convenient and applicable to the separation of ganglioside from animal tissue materials. Using the method to prepare ganglioside can avoid using a great deal of toxic organic solvent, and the method also has the advantages of energy saving, environmental protection, short production period, low cost and high yield rate. The dosage of organic solvent can be effectively reduced, and the tissue extraction rate of ganglioside is increased.

An extract of animal's brain is prepared by use of composite enzyme and can play the role of increasing the adaptive capacity to mental stress. A food for increasing the adptive capacity to mental stress contains said extract and 20 other components including VA, VE, folic acid, glutamine, etc.

The present invention is to provide a method of treatment which inhibits proliferation and invasion of brain tumor cells in developing animal brain tumor cells, and a method for measuring proliferation / invasion activity of brain tumor cells, in the brain tumor cells, preferably, in glioblastoma. Proliferation and invasion of brain tumor cells are inhibited by regulating Ca2+ permeability by glutamate receptor subunits in developing animal brain tumor cells. Regulation of Ca2+ permeability by glutamate receptor subunits can be conducted by introducing the GluR2 gene into AMPA-type glutamate receptors in brain tumor cells. Further, the present invention provides a method for measuring proliferation / invasion activity of brain tumor cells by detecting / measuring the expression of glutamate receptor subunits in developing animal brain tumor cells.

Method and device for determining target brain segments in human or animal brainsAn embodiment of the invention relates to a method for determining at least one target brain segment of a human or animal brain for stimulation or manipulation of a specific brain function, said method comprising the steps of:(a) receiving first data of a reference brain, said first data defining a reference network consisting of functionally correlated brain segments that perform said specific brain function,(b) identifying the corresponding network in the human or animal brain,(c) receiving second data of said reference brain, said second data comprising a description of at least one location in the reference network, said location defining a target brain segment for stimulation or manipulation of said specific brain function in the reference brain, and(d) identifying at least one corresponding location in the corresponding network of the human or animal brain based on said description, said at least one corresponding location defining said at least one target brain segment of said human or animal brain.

The invention relates to the novel use of pharmaceutical preparations in the field of pharmacy, more specifically the use of barrenwort flavones and barrenwort glycosides monomers as active components in preparing medicament for preventing and treating suppression. Pharmaceutical test shows that, the barrenwort flavones and barrenwort glycosides monomer can appreciably shorten rat's tail suspension time and forced swimming standby time, reverse the decrease of mouse sucrose consumption caused by CMS, suppress animal brain monoamines oxidation enzyme an and B, thus achieving the purpose of suppression resistance.

The invention relates to a method for extracting cholesterol from animal brain dry powder with ultrasonic waves. Animal brain dry powder is used as raw materials, and the method comprises the steps of: I, dipping before extraction; II, ultrasonic extraction; III, concentration and recovery of acetone; IV, hydrolysis, cooling and crystallization; V, dissolution and crystallization; VI, recrystallization; and VII making of finished products. The method has the advantages that first, the used quantity of a dipping solution for the animal brain dry powder is reduced (5 times of quantity is used to the largest); second, the dipping time is enormously shortened (the dipping lasts for 9h furthest); third, high-purity cholesterol (with purity of 95%-97%) can be obtained by three times of crystallization on the premise of guaranteeing high yield (up to 8.5%-10%), and multiple reflux and heating ensure no toxic organic solvent left.

The invention discloses a cerebroprotein hydrolysate and a preparation method thereof, and belongs to the technical field of medicines. The preparation method comprises the following step: carrying out enzymolysis on an animal brain tissue by adopting pepsase and compound flavourzyme in a mass ratio of (6:1)-(2:1) at 37 DEG C under a corresponding enzymolysis pH value controlled at 1-5 for 2-10 hours, thereby obtaining the cerebroprotein hydrolysate, wherein cerebroprotein hydrolysate is a composition of oligopeptide with molecular weight of 1000 and polypeptide. And moreover, macromolecule impurities are removed by centrifuging, and impurities such as pyrogen are filtered and removed by a nanofiltration membrane. The finally obtained cerebroprotein hydrolysate has apyrogeneity, high oxidative-stress-resisting activity and good repair capacity on nerve cells.

The invention discloses an application of geniposide which is used as an acetylcholin esterase inhibitor in a medicament for treating and preventing diseases with acetylcholin esterase activity abnormity. The invention relates to geniposide which directly inhibits activity of the acetylcholin esterase, inhibits activity of acetylcholin esterase in a cultured nerve cell, and inhibits activity of the acetylcholin esterase in a transgenic animal brain with Alzheimer's disease. The geniposide can bring inhibition effect on the activity of the acetylcholin esterase into play by virtue of the way, but is not limited to the way, and the geniposide possibly has a potential treatment value on the Alzheimer's disease by inhibiting the acetylcholin esterase.

The invention discloses an electrode applicable to an animal brain area and adjustable in implantation depth. The electrode comprises a microelectrode and an electrode wire depth adjusting device. The electrode wire depth adjusting device comprises an upper supporting plate, a lower supporting plate and an adjusting plate. The upper and lower supporting plates are connected integrally through two guiding upright columns arranged at an interval. A screw of a bolt structure is perpendicularly arranged between the two guiding upright columns. The lower end of the screw is rotationally arranged in a shaft sleeve on the lower supporting plate, and the upper end is rotationally arranged in a step hole formed in the upper supporting hole. The adjusting plate is arranged on the two guiding upright columns in a sliding and sleeving manner by means of through holes. The middle part of the adjusting plate is in threaded transmission connected with the screw through a screw joint member. A stainless steel positioning pipe penetrates through the lower supporting plate and is vertically fixed downwards. The microelectrode is fixed on the adjusting plate, and electrode wires of the microelectrode are vertically inserted downwards into the stainless steel positioning pipe at intervals. The electrode provided by the invention solves the problem that depth adjusting modes of detection electrodes and exciting electrodes are not universal, is flexible in form and is wide in application.

The invention provides a method and a device for establishing a superficial sensory disturbance animal model. The method comprises the following steps: (1) establishing operant conditioned reflex avoiding noxious stimulation for animals; (2) preparing hemiplegia models for animals which establish conditioned reflex; and (3) only exposing the affected limbs of hemiplegia animals, and screening superficial sensory disturbance. The device is suitable for the method for establishing the operant conditioned reflex avoiding shock stimulation for animals, which comprises a box body, a circuit control device, and a shock device and a power-off device which are positioned at the bottom of a box. The method for establishing the superficial sensory disturbance animal model provided by the invention can accurately record the performance of animals in the process of experiments in detail and facilitate the collation and statistics of data, can establish a superficial sensory disturbance model of affected limbs of the animals after stroke, has the advantages of simple operation, accurate data, easy training for animals, short test cycle and the like; and the corresponding device has simple structure and easy operation.

The invention relates to a method for extracting brain-nourishing bioactive peptide through adopting animal brain tissue, in particular to a method for extracting brain-nourishing bioactive peptide from the brain tissue of livestock. The method adopts the following steps of animal brain tissue obtaining, vacuum suction of animal brain tissue, directional biological enzymolysis, bioactive peptide separation of animal brain, low-temperature concentration, molecular embedding and spray drying so as to make brain-nourishing bioactive peptide finished product. The method has the advantage that: bioengineering technology is adopted to promote the development of livestock product processing industry towards a high-tech and high-added value industry. Moreover, the method provides a new opportunity to make animal brain resources which is regarded as having low biological value into brain-nourishing bioactive peptide with biological activity and meeting better the requirements of human physiological function for health-care nutrition under the action of biological enzyme engineering and ultramicromembrane separation engineering, etc.

The invention discloses a microelectrode propelling device and a microelectrode propelling method. The device comprises a gasket, a box body, a nut, screws, a slide block and a bottom plate. The propelling method comprises the following steps that: the periphery of the bottom plate is arranged into a step shape, the bottom plate is positioned on a skull by positioning screws through two semicircular holes serving as positioning reference on the bottom plate and positioning holes at corresponding positions on the skull, and the bottom plate and the skull are bonded and fixed through dental cement; the bottom plate is connected with the box body through concavely and convexly matched grooves; the slide block is arranged inside a slideway of the box body, an array plate is arranged on a stepped groove of the slide block so as to fix a microelectrode, the position of a hole on the array plate is the position of intersecting axles of a sagittal plane and a coronal plane at a brain region-specified space encoding position, a horizontal position of the array plate is a propelling surface of a propeller, and the propeller performs electrical brain stimulation and encephalographic record on the whole brain region. The propelling device has the characteristics that: the operation is simple and the structure is light, and the device is suitable for labs for researching animal brain regions.

The invention discloses an animal injure experimental device. The animal injure experimental device comprises an animal fixing platform. A support, a hydraulic conveying mechanism and a driving mechanism are arranged on the animal fixing platform, a platform body is arranged at the lower portion of the support, manual fixing clamps, an automatic clamp and an animal lower limb fixing device are arranged on the platform body, a beating hammer, an electromagnetic angle disc and an angle sensor are arranged at the upper portion of the support, the beating hammer comprises a hammer head and a hammer handle, the hammer head is installed at the top end of the hammer handle, the back end of the hammer handle is connected with the angle sensor, a guide rod is arranged on the hammer handle, a guide groove is formed in the electromagnetic angle disc, and the guide rod is located in the guide groove; the hydraulic conveying mechanism comprises a fluid diversion valve and a hydraulic pump, and the fluid diversion valve is connected with the hydraulic pump through a pipe. The animal injure experimental device can be used for animal brain injury experiments and animal muscle strain experiments.

The invention discloses an animal brain tissue section microscopic image segmentation method, and relates to the technical field of microscopic image processing, and the method comprises the steps: (1) carrying out the preprocessing of a microscopic image; (2) carrying out wavelet transformation on the microscopic image; (3) performing GA-FCM image segmentation; (4) reconstructing the wavelet of the microscopic image; (5) performing quantitative evaluation on the enhanced image; (6) analyzing whether the evaluation index reaches a preset index; and (7) outputting a microscopic image segmentation result: firstly, processing the microscopic image by virtue of wavelet transform to obtain high-frequency and low-frequency subspace images, and then respectively segmenting the high-frequency subspace image and the low-frequency subspace image, so that the high-frequency component and the low-frequency component of the microscopic image are effectively segmented, and GA-FCM is adopted to realize the effective segmentation of the microscopic image, and evaluates the segmentation effect by means of the quantitative index of objective evaluation, thereby ensuring the segmentation accuracy ofthe microscopic image, and meeting the requirement of subsequent three-dimensional reconstruction of the brain tissue structure.

The invention discloses a preparation method of a microelectrochemical sensor for detection of dopamine concentration in an animal brain, wherein a silicon-based microelectrode array is used as an electrode to be modified; firstly, an electrodeposition method and a chemical etching method are used to deposit a layer of porous gold-platinum nanoparticles on the electrode sites of the silicon-basedmicroelectrode array, then an electropolymerization is used to deposit a layer of perfluorosulfonic acid (Nafion) film on the electrode sites modified with the gold-platinum nanoparticles to obtain the microelectrochemical sensor for detection of dopamine concentration. The prepared microelectrochemical sensor for detection of dopamine concentration in the animal brain is implanted into striatum nuclei of the animal brain, and is connected as a working electrode to an electrochemical workstation to realize detection of dopamine concentration in the animal brain by detecting of a dopamine electrochemical signal, and the microelectrochemical sensor is simple in preparation, high in sensitivity, strong in selectivity, and small in volume.

The invention discloses a compound soft capsule containing an acanthopanax extract for strengthening the brain and a preparation method of the compound soft capsule. The method comprises the following steps: orderly dissolving the acanthopanax extract, mixed phospholipid and vegetable oil into an ethanol solution of poloxamer, removing ethanol to obtain content of the soft capsule; and encapsulating the content into a soft capsule shell, wherein the dosage of the mixed phospholipid is 90-240 parts by weight, the dosage of the vegetable oil is 140-300 parts by weight and the dosage of the poloxamer is 10-50 parts by weight relative to 100 parts of acanthopanax extract, the mixed phospholipid is prepared from an animal brain extract and adding ingredients, the weight ratio of the animal brain extract to the added ingredients is 1:(2-6) and the added ingredients comprise phosphatidylserine and / or dilinoleoyl phosphatidylcholine. The compound soft capsule for strengthening the brain, which is disclosed by the invention, can be effectively used for assisting improvement of memory, improvement of sleeping, and prevention of senile dementia, and has a good health-care effect.