Phosphatidylserine is extracted from animal brains by: drying animal brains, smashing them into granules, putting propanone into the granules, stirring and extracting, separating propanone supernatant liquid contained cholesterine and oils from the brain granules, steaming the residues to be dried, adding n-hexane or alcohol or ammonia water alcohol into steamed dry residues, stirring and extracting, separating extracting liquid contained phospholipid compositions, steaming the residues again, putting ether into steamed dry residues, stirring, freezing and defreezing, filtering to remove deposits, removing the remained phospholipid further, adjusting supernatant liquid pH=3-6, steaming to remove ether, and steaming to dry crude phosphatidylserine again, dissolving crude phosphatidylserine dry powders in n-hexane, adding sodium acetate alcohol liquid, stirring, settling to be laminated, removing the supernatant contained inositol phospholipid, finally, steaming to dry the n-hexane in supernatant liquid to obtain high pure phosphatidylserine under reduced pressure.