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Extraction, purification process for ganglioside

A technology of gangliosides and production methods, which is applied in organic chemistry, pharmaceutical formulations, bulk chemical production, etc., can solve the problems of high toxicity of organic solvents, long cycle time, and great harm to operators, so as to improve tissue extraction rate, The effect of short production cycle and low cost yield

Active Publication Date: 2008-09-24
JILIN YINGLIAN BIOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But no matter above-mentioned which kind of method, all because organic solvent toxicity is big, usage amount is big, operator's hazard is big, energy consumption is high, environmental pollution is serious, production process is complicated, cycle is long, and the restricting factor such as low cost of yield is difficult to overcome and greatly greatly. It hinders the industrialization and application of gangliosides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0013] 1. Raw material processing: 10Kg of animal brain tissue is cleaned to remove impurities such as blood and bone fragments, and crushed. spare.

[0014] 2. Feeding: Open the top cover of the extraction tank, and put the material into the stainless steel wire mesh basket in the extraction tank.

[0015] 3. Heating: The buffer tank, extraction tank and separation tank temperature controller controls the temperature to set 45°C. Keep the entire system at a constant temperature.

[0016] 4. Pressurized extraction: CO in cylinder 2 (Purity > 99%) enters the booster pump to pressurize to 30MPa after being filtered by the filter, and exports CO 2 After being preheated by the heat exchanger, it enters the buffer tank. CO 2 In the buffer tank, the pressure is stabilized and the temperature is raised to the operating pressure and temperature, and then heated to the operating temperature or constant temperature through a heat exchanger to form a supercritical state, enter the e...

Embodiment approach 2

[0020] 1. Raw material processing: 10Kg of animal brains are cleaned to remove impurities such as blood and bone fragments, and crushed. spare.

[0021] 2. Feeding: Open the top cover of the extraction tank, and put the material into the stainless steel wire mesh basket in the extraction tank.

[0022] 3. Heating: The buffer tank, extraction tank and separation tank temperature controllers control the temperature and set the temperature to 50°C. Keep the entire system at a constant temperature.

[0023] 4. Pressurized extraction: CO in cylinder 2 (Purity > 99%) enter the booster pump to pressurize to 35MPa after being filtered by the filter, and export CO 2 After being preheated by the heat exchanger, it enters the buffer tank. CO 2 In the buffer tank, the pressure is stabilized and the temperature is raised to the operating pressure and temperature, and then heated to the operating temperature or constant temperature through a heat exchanger to form a supercritical state...

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Abstract

The present invention relates to a method for extracting and purifying ganglioside, which separates out neutral lipid impurity components from the preprocessed animal brain tissues by supercritical extraction under the function of supercritical fluid (SCF), entrainer is then added into the supercritical fluid (SCF) to unceasingly extract animal brain powder without neutral lipid impurities, and polar ganglioside components are selectively resolved to produce the ganglioside (GLS), which then is treated by purification process, such as adsorption chromatography separation or ion exchanger separation, etc., to produce high-purity ganglioside components. The operation of the method is convenient and applicable to the separation of ganglioside from animal tissue materials. Using the method to prepare ganglioside can avoid using a great deal of toxic organic solvent, and the method also has the advantages of energy saving, environmental protection, short production period, low cost and high yield rate. The dosage of organic solvent can be effectively reduced, and the tissue extraction rate of ganglioside is increased.

Description

technical field [0001] The invention relates to a production method of gangliosides, in particular to a method for extracting and purifying gangliosides in the field of medicine. technical background [0002] Ganglioside (GLS) is a class of acidic glycosphingolipids present on the surface of cell membranes. It was discovered in nerve cells as early as 1940 and got its name. It is most abundant in the nervous system and also exists in the spleen. In red blood cells, it is the most complex class of acidic glycosphingolipids. [0003] GLS includes monosialoganglioside GM, disialoganglioside GD, etc. Although the content in the cell membrane is very small, it has many special biological functions. As an important component of the cell membrane, GIS can be a specific receptor for certain biologically active substances such as neurotransmitters, hormones, viruses, interferons, cholera toxins, etc., and can mediate or regulate these biologically active substances. It has been con...

Claims

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Application Information

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IPC IPC(8): C07H15/04C07H15/10A61K35/30
CPCY02P20/54
Inventor 芦志刚
Owner JILIN YINGLIAN BIOPHARML
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