Animal brain extract and food component composition for enhancing psycological stress adaptation
A technology of animal brain and extract is applied in the field of nutritional food to achieve the effects of reducing abnormal reactions, reducing hypertension, reducing the occurrence of psychological abnormalities and stress injuries
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Embodiment 1
[0048] Animal brain extract, made by the following method:
[0049] (1) Choose healthy pigs, take out fresh brain tissue immediately after slaughter, put it in boiling water at 100°C for 10 minutes, take it out and cool it naturally, and then store it in a -20°C environment for refrigeration;
[0050] (2) Weigh 100 grams of animal brain, mince it, add 200 grams of sterile distilled water, stir into a slurry, place it in a reactor, and adjust the pH of the slurry to 6.5;
[0051] (3) Add mixed enzymes to the slurry, stir evenly, and carry out hydrolysis reaction at a temperature of 50° C. for 4 hours. The mixed enzymes are composed of Flavourzyme, Protamex and 1398 neutral protease (Flavourzyme and Protamex are produced by Danish NOVONORDISK company) , add the Flavourzyme of 900LAPU (LAPU / g is the active unit of the enzyme Flavourzyme), the Protamex of 1350AU (AU / g is the active unit of the enzyme Protamex), add 1.1 grams of 1398 neutral protease;
[0052] (4) Add dilute hydro...
Embodiment 2
[0055] Animal brain extract, made by the following method:
[0056] (1) Choose healthy cattle, take out fresh brain tissue immediately after slaughter, put it in boiling water at 100°C for 5 minutes, take it out and cool it naturally, and store it in a -20°C environment for refrigeration;
[0057] (2) Weigh 100 grams of animal brain, mince it, add 100 grams of sterile distilled water, stir into a slurry, place it in the reactor, and adjust the pH of the slurry to 4.5;
[0058] (3) Add a mixed enzyme to the slurry, stir evenly, and carry out a hydrolysis reaction at a temperature of 70° C. for 2 hours. The mixed enzyme is composed of Flavourzyme, Protamex and 1398 neutral protease. Add 200 LAPU of Flavourzyme and 3000 AU of Protamex, Add 0.8 grams of 1398 neutral protease;
[0059] (4) add lactic acid to make the pH be 4, stop the hydrolysis reaction;
[0060] (5) After the hydrolyzate is naturally cooled, the pH is adjusted to 6 with a dilute alkali solution, and freeze-drie...
Embodiment 3
[0062] Animal brain extract, made by the following method:
[0063] (1) Choose healthy sheep, take out fresh brain tissue immediately after slaughter, put it in boiling water at 100°C for 20 minutes, take it out and cool it naturally, and then store it in a -20°C environment for refrigeration;
[0064] (2) Weigh 100 grams of animal brain, mince it, add 300 grams of sterile distilled water, stir into a slurry, place it in a reactor, and adjust the pH of the slurry to 8.5;
[0065] (3) Add a mixed enzyme to the slurry, stir evenly, and carry out a hydrolysis reaction at a temperature of 30° C. for 6 hours. The mixed enzyme is composed of Flavourzyme, Protamex and 1398 neutral protease. Add 2000 LAPU of Flavourzyme and 300 AU of Protamex, Add 1.4 grams of 1398 neutral protease;
[0066] (4) add lactic acid to make the pH be 4, stop the hydrolysis reaction;
[0067] (5) After the hydrolyzate is naturally cooled, the pH is adjusted to 8 with a dilute alkali solution, and freeze-d...
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