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Broad spectrum bacilliform virus insecticide made of substitution host

A baculovirus and pesticide technology, which can be used in pesticides, biocides, plant growth regulators, etc., can solve the problems that the original host cannot proliferate in large quantities, and the original host cannot be artificially raised, and achieves good control effect, Low cost and wide insecticidal spectrum

Active Publication Date: 2008-05-14
JIANGXI NEW DRAGON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a method for producing the broad-spectrum baculovirus Brassica oleracea nucleopolyhedrosis virus by using an alternative host, beet armyworm or cotton bollworm, so as to solve the problem that the original host of cabbage armyworm nucleopolyhedrosis virus cannot Artificial breeding, the problem that the original host cannot be multiplied in large quantities, thus providing a broad-spectrum rod-shaped insecticide that can control various important pests such as diamondback moth, beet armyworm, cotton bollworm, tobacco budworm, cotton red bollworm and cabbage armyworm. virus insecticide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] The broad-spectrum baculovirus was Cabbage Spodoptera nuclear polyhedrosis virus, and the alternative host was Cotton bollworm. The 4th instar cotton bollworm larvae fed with artificial diet were infected with 3 million PIB / mL virus suspension to produce Cabbage Spodoptera nuclear polyhedrosis virus. By weight, the artificial feed formula for the cotton bollworm larvae is: 6% each of soybean flour, wheat flour, and corn flour, 1.2% yeast powder, 1% agar strip, 0.1% each of Vc and compound Vb, sorbic acid and paraben B Each ester is 0.03%, and the balance is tap water. 5-8 days after the virus infects the alternative host, collect the corpses of dead insects caused by the virus and process them into a virus suspension of 20 billion PIB / mL.

[0022] Then, according to the weight ratio, add 20 billion PIB / ml virus suspension 10%, 60nm silicon dioxide 0.3%, aromatic amino acid tryptophan 0.12%, refined fluorescent whitening agent 1.0%, sodium lignosulfonate 0.2% %, 0.05% o...

example 2

[0024] The broad-spectrum baculovirus is Cabbage Spodoptera nuclear polyhedrosis virus, and the alternative host is Beet Spodoptera. The 4th instar Beet Spodoptera larvae raised on artificial diet were infected with 500,000 PIB / mL virus suspension to produce Cabbage Spodoptera nuclear polyhedrosis Virus. By weight, the formula of artificial feed for the beet armyworm larvae is: 10.5% of soybean powder, 5.5% of wheat bran and corn flour, 1.2% of yeast powder, 0.4% of sucrose, 6% of carrageenan, 0.1% of Vc and compound Vb , 0.02% each of sorbic acid and ethylparaben, 0.5% chlortetracycline, and the balance in tap water. 4-7 days after the virus infects the alternative host, collect the corpses of dead insects caused by the virus and process them into a virus suspension of 20 billion PIB / mL.

[0025] Then, according to the weight ratio, 7.5% of 20 billion PIB / ml virus suspension, 0.15% of 60nm silicon dioxide, 0.14% of aromatic amino acid tyrosine, 0.8% of refined fluorescent wh...

example 3

[0027] The broad-spectrum baculovirus was Cabbage Spodoptera nuclear polyhedrosis virus, and the alternative host was Cotton bollworm. The 3rd instar cotton bollworm larvae fed with artificial diet were infected with 1 million PIB / mL virus suspension to produce Cabbage Spodoptera nuclear polyhedrosis virus. The artificial feed formula of this cotton bollworm larva is identical with example one. 4-9 days after the virus infects the alternative host, collect the corpses of dead insects caused by the virus and process them into a virus suspension of 20 billion PIB / mL.

[0028] Then, according to the weight ratio, add 12% of 20 billion PIB / m1 virus suspension, 0.6% of 60nm silicon dioxide, 0.11% of aromatic amino acid tryptophan, 1.0% of refined fluorescent whitening agent, and 0.4% of sodium lignosulfonate %, 0.05% of chlorfluazuron, 1.2% of glycerin and the balance of sterile water, and then stirred evenly and subpackaged to obtain a milky white suspension product.

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Abstract

The invention provides a broad-spectrum rod-shaped viral pesticide produced by substitute hosts, the components and the weight proportion of which are: 20 billion PIB / ml virus suspension 2.5 to 15 percent, 60nm silicon dioxide 0.1 to 0.5 percent, aromatic amino acid 0.1 to 0.15 percent, refined fluorescent whitening agent 0.5 to 1 percent, sodium lignin sulfonate 0.1 to 1 percent, chlorfluazuron 0.01 to 0.1 percent, glycerol 1.0 to 2.0 percent, and remaining no sterilized water; the virus suspension is received by the infection of nuclear polyhedrosis virus of cabbage armymoth and the substation for the host---cotton bollworm or beet armyworm. The pesticide not only maintains the biological pesticide properties of viral pesticides and has no harms to human beings and higher animals, but also is suitable for the the massive employments on vegetables, and has the wide insect disinfestation spectrum. Therefore, a broad-spectrum viral pesticide can prevent and control various harmful insects while with only a rod-shaped virus. The invention has the advantages of good insecticidal effect, fast insecticidal speed, and very good application prospect.

Description

technical field [0001] The invention relates to a biological insecticide preparation, in particular to a broad-spectrum baculovirus insecticidal suspension produced by a substitute host, which is suitable for diamondback moth, beet armyworm, cotton bollworm, tobacco budworm, cotton red bollworm and cabbage nightworm Biological control of moths and other pests. Background technique [0002] Baculoviruses, including nuclear polyhedrosis viruses and granuloviruses, are viruses that specifically infect insects and other arthropods. Nuclear polyhedrosis virus, like HIV in humans, can make infected pests suffer from incurable diseases. When the pests eat the polyhedrosis virus into their stomachs, the polyhedrin protein dissolves immediately when it encounters the strong alkaline digestive juice of the insect midgut, releasing the virus particles, which quickly invade the midgut cells and amplify by orders of magnitude in the nucleus. The amplified virus particles infect the pes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P7/04A01N47/34
Inventor 张忠信姚立刘海良孙修炼彭辉银
Owner JIANGXI NEW DRAGON BIOTECH
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