Method for preventing and controlling tobacco mosaic virus disease
A tobacco mosaic virus, tobacco technology, applied in the directions of botanical equipment and methods, disinfectants, chemicals for biological control, etc., can solve the problems of high cost, difficult realization, difficult results, etc. Simple, convenient effect in field application
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Embodiment 1
[0010] Embodiment 1: Utilize the phenol chloroform method to extract the RNA genome of tobacco mosaic virus; With the genome RNA of tobacco mosaic virus as template, reverse transcription obtains the cDNA of tobacco mosaic virus motor protein; Utilize PCR technology to isolate tobacco mosaic virus movement A fragment of 21-23bp in the range of 150-800bp on the protein cDNA; the obtained above-mentioned fragment is cloned into a plasmid vector capable of expressing RNA; the cloned fragment is expressed in the corresponding E. coli (E.coli) lacking RNase RNA; smash the cells expressing the purpose RNA, and make it into a suspension solution; the content of RNA in the solution is 2-20ng / ml, and the photoprotectant aromatic amino acid tryptophan accounts for 0.01-0.1% of the volume of the solution, dispersed Agent 1-2%, glycerin 2-5%, sterile water 96.99-92.9%. The dispersant is L602 or LH656. During preparation, glycerin, aromatic amino acid tryptophan, dispersant and sterilized...
Embodiment 2
[0011] Embodiment 2: In the present embodiment, the composition of suspension solution is: the content of RNA is 5ng / ml, photoprotectant aromatic amino acid tryptophan 0.05%, L602 dispersant 1.5%, glycerin 3.5%, sterile water 94.95% %. Others are the same as in Example 1.
Embodiment 3
[0012] Embodiment 3: In the present embodiment, the composition of suspension solution is: the content of RNA is 15ng / ml, photoprotectant aromatic amino acid tryptophan 0.08%, LH656 dispersant 1.8%, glycerol 4%, sterile water 94.72% %. Others are the same as in Example 1.
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