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Spectinomycin, streptomycin, gentamycin and neomycin detection test strip and method thereof

A technology of spectinomycin and gentamicin, applied in the field of test strips for detecting spectinomycin, gentamicin and neomycin, and streptomycin, which can solve the problems of lack of drugs and so on

Active Publication Date: 2014-04-09
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is a lack of technology and application in the field of immunoassays that can simultaneously detect the four drugs of spectinomycin, streptomycin, gentamicin and neomycin

Method used

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  • Spectinomycin, streptomycin, gentamycin and neomycin detection test strip and method thereof
  • Spectinomycin, streptomycin, gentamycin and neomycin detection test strip and method thereof
  • Spectinomycin, streptomycin, gentamycin and neomycin detection test strip and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1, detect the composition of the test paper strip of spectinomycin, streptomycin, gentamycin and neomycin

[0066] 1. Freeze-dried microwell strips with gold-labeled antibodies

[0067] Microwell reagents are housed in the microwell strip, wherein 1 is a microwell strip, and 2 is freeze-dried spectinomycin specific antibody-colloidal gold marker, streptomycin specific antibody-colloidal gold marker, Qingda Microporous reagents for mycin-specific antibody-colloidal gold markers and neomycin-specific antibody-colloidal gold markers;

[0068] The microwell strip containing the micropore reagent has a microwell plug 3 ( figure 2 and image 3 );

[0069] The freeze-dried spectinomycin monoclonal antibody-colloidal gold marker, streptomycin monoclonal antibody-colloidal gold marker, gentamicin monoclonal antibody-colloidal gold marker and neomycin monoclonal antibody in the microwell strip Microwell reagents for cloning antibody-colloidal gold markers, the lyo...

Embodiment 2

[0077] The preparation method of test strips described in embodiment 2 and embodiment 1

[0078] 1. Preparation of test strips

[0079] The preparation method of the test strip mainly comprises the following steps:

[0080] 1) Preparation of freeze-dried spectinomycin monoclonal antibody-colloidal gold label, streptomycin monoclonal antibody-colloidal gold label, gentamicin monoclonal antibody-colloidal gold label and Neomycin in microwell strips A microwell reagent for a prime monoclonal antibody-colloidal gold label and cap the microwell strip with a microwell stopper.

[0081] 2) Prepare a detection line (T1) coated with spectinomycin-carrier protein conjugate, a detection line (T2) coated with streptomycin-carrier protein conjugate, and a gentamicin-carrier protein conjugate coated The reaction membrane of the detection line (T3) of the conjugate, the detection line (T4) coated with the neomycin-carrier protein conjugate, and the quality control line (C) coated with goat...

Embodiment 3

[0160] Embodiment 3, the application of test strip

[0161] One, detect spectinomycin, streptomycin, gentamicin and neomycin in milk with test strips described in Example 1

[0162] The test strip of the present invention can detect milk samples. The general detection method is as follows:

[0163] 1. Detection method

[0164] Remove the microporous plug covering the microporous strip, and drop the sample solution to be detected into the microporous strip containing the microporous reagent to obtain a mixed solution 11 of the microporous reagent and the sample solution. After mixing, incubate 5min, insert the test paper 12 with the marked end of the MAX line into the microporous strip, and watch the result within 5min, such as Figure 7 shown.

[0165] 2. Determination of test results

[0166] If the content of spectinomycin or streptomycin or gentamicin or neomycin in the sample liquid to be tested is high, the spectinomycin or streptomycin or gentamicin or new It can b...

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Abstract

The invention discloses a spectinomycin, streptomycin, gentamycin and neomycin detection test strip and a method thereof. The test strip comprises micro-porous strips, micro-porous reagents, a test paper and micro-porous plugs, the micro-porous reagents are lyophilized colloidal gold labeled spectinomycin, streptomycin, gentamycin and neomycin specific antibodies respectively, the test paper is formed by sequentially connecting a sample absorption pad, a reaction film, a water absorption pad, a protection film and a substrate, the reaction film comprises four detection lines and a quality control line, the four detection lines are coated with a spectinomycin-carrier protein conjugate, a streptomycin-carrier protein conjugate, a gentamycin-carrier protein conjugate and a neomycin-carrier protein conjugate respectively, and the quality control line is coated with an antiantibody. The method for detecting the spectinomycin, streptomycin, gentamycin and neomycin by using the test strip has the advantages of simplicity, rapidness, perceptual intuition, accuracy, portability, wide application range, low cost, and easy popularized use.

Description

technical field [0001] The invention relates to a test strip and a method for detecting spectinomycin, streptomycin, gentamycin and neomycin. Background technique [0002] Spectinomycin, also known as spectinomycin, spectinomycin, spectinomycin, etc., is an aminocycloalcohol antibiotic produced by Streptomyces grandis, which is effective against Gram-negative bacteria (such as Brucella, Kray Bacteria, Proteus, Salmonella, Pasteurella, etc.) have strong effects on Gram-positive bacteria (Streptococcus, Staphylococcus, etc.), and have certain effects on Mycoplasma. It is a new type of special treatment Antibiotics for gonorrhea, which have a strong antibacterial effect on Neisseria gonorrhoeae. It can promote animal growth and is a good animal feed additive. It is widely used in animal husbandry as veterinary medicine. Some unscrupulous traders are driven by economic interests to abuse antibiotics, leaving a large amount of antibiotics in animal products. If you eat animal-d...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/531G01N33/558G01N33/577
Inventor 何方洋冯才伟张禹付军权吴鹏杨秀贤李勇邵晓雪
Owner BEIJING KWINBON BIOTECH
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