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Freezing diluent formula for improving quality of Holstein bull frozen-thawed X-spermatozoa

A technology for freezing diluents and sperm, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., and can solve problems such as high sperm mortality, low pregnancy rate, and cumbersome and complicated procedures

Active Publication Date: 2013-02-06
NORTHWEST A & F UNIV
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Problems solved by technology

[0032] Compared with the pre-treatment of ordinary semen freezing, the processing method of X sperm is cumbersome and complicated, the processing time is relatively long, and the sperm mortality rate is high during the processing process, resulting in low sperm motility and low pregnancy rate after mating The object of the present invention is to provide a novel semen diluent formula for improving the frozen-thawed semen quality and fertilization rate of Holstein cattle X sperm

Method used

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  • Freezing diluent formula for improving quality of Holstein bull frozen-thawed X-spermatozoa

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Embodiment Construction

[0041] According to the technical scheme of the present invention, the present embodiment provides a kind of frozen diluent for improving the frozen quality of Holstein bull X sperm, and the prepared frozen diluent contains the following substances per 100 milliliters:

[0042] Sodium citrate: 0.9-1.2 grams, sucrose: 3-6 grams, low-density lipoprotein: 16-18 mg, glycerin: 4-7 ml, vitamin E: 500 mg-700 mg, vitamin B 12 : 100 mg to 200 mg, spectinomycin: 0.35 mg, gentamicin: 70,000 to 80,000 units (IU), make up 100 ml with distilled water.

[0043] Its preparation method is:

[0044] 1. Weigh raw materials: sodium citrate, sucrose, low-density lipoprotein, glycerin, vitamin E, vitamin B12, distilled water.

[0045] 2. The above sodium citrate, sucrose, low-density lipoprotein, vitamin E, vitamin B 12 , glycerol, and distilled water to prepare the base liquid, stir with a magnetic stirrer for 60 minutes, and store in a 4°C refrigerator overnight for later use.

[0046] 3. Add ...

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Abstract

The invention discloses a freezing diluent formula for improving the quality of Holstein bull frozen-thawed X-spermatozoa. The freezing diluent comprises the following ingredients per 100 ml: 0.9-1.2 g of sodium citrate, 3-6 g of sucrose, 16-18 mg of low density lipoprotein, 4-7 ml of glycerol, 500-700 mg of Vitamin E, 100-200 mg of Vitamin B12, 0.35 mg of spectinomycin, 70000-80000 International Unit (IU) of gentamicin and the balance of distilled water for making up 100ml. The experimental data show that the freezing diluent improves the activity and the acrosome intact rate of frozen-thawed X-spermatozoa, and improves the quality of frozen-thawed spermatozoa.

Description

technical field [0001] The invention relates to an improved technique for artificial insemination of dairy cows, in particular to a formula of frozen semen diluent for improving the frozen quality of Holstein bull X sperm. Background technique [0002] The successful freezing of domestic animal semen has had an extremely profound impact on the development of artificial insemination technology. Firstly, it has laid the foundation for expanding the utilization rate and prolonging the utilization time of excellent breeding males, and secondly, it has provided a broad space for the development of artificial insemination and cryobiology. . Because frozen semen is more convenient in transportation, no matter in terms of time or space, the mating efficiency of male animals can be fully exerted, and the pace of livestock breed improvement has been accelerated. However, animals of different sexes can meet people's needs in different aspects, so people will intervene in the normal re...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 赵宪林昝林森胡建宏田万强王韦华郝瑞杰桂林生
Owner NORTHWEST A & F UNIV
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