300 results about "Monocotyledon" patented technology

Methods of increasing nitrogen utilization efficiency in monocot plants through genetic modification to increase the levels of alanine aminotransferase expression and plants produced there from are described. In particular, methods for increasing the biomass and yield of transgenic monocot plants grown under nitrogen limiting conditions compared to non-transgenic plants are described. In this way, monocot plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.

The invention discloses a CRISPR / Cas9 technology-based plant gene knockout vector and an application thereof. The plant gene knockout vector is monocotyledon CRISPR / Cas9 knockout vector pCAMBIA1300DM-OsU3(BspQ I)-Cas9, and contains a BspQ I insertion site. The CRISPR / Cas9 plant knockout binary vector pCAMBIA1300DM-OsU3(BspQ I)-Cas9 commonly constructed by an sgRNA-OsU3 sequence composed of the BspQ I insertion site and a 2x35s-hSpCas9-ter sequence can realize high-efficiency rapid construction of the monocotyledon gene knockout vector through a one-step restriction and ligation method; and the CRISPR / Cas9 knockout vector pCAMBIA1300DM-OsU3(BspQ I)-Cas9 has the characteristics of high targeting efficiency and low off-target efficiency, and can be applied to plant target gene knockout or applied to plant gene editing in a kit form.

The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.

The invention discloses a special expressing promoter of plant endosperm and appliance, which is characterized by the following: 1) possessing DNA sequence of sequence 1 in sequence table; 2) possessing 70% or above 70% homologous property with limited DNA sequence of sequence 1 in sequence table; possessing DNA sequence with same function; 3) possessing crossing nucleic acid sequence with limited NDA sequence of sequence 1 in sequence table under high strict condition. This promoter can start special expression of external source gene in plant endosperm, which is fit for any plant with endosperm such as monocotyledon or dicotyledon.

The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, and to methods of making these isolated blood proteins and to therapeutic compositions comprising them.

The invention relates to an alfalfa stress response gene MsNAC3 and an application thereof. A novel NAC (N-Acetyl Cysteine) family related gene is cloned and authenticated from an alfalfa genome by using an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technology and is named as MsNAC3 with a nucleotide sequence as shown in SEQ ID No.11, and a fusion expression vector containing an enhanced green fluorescent protein (EGFP) gene is constructed. The expression pattern of the gene MsNAC3 in alfalfa and the relation between the gene and adversity stress (cold damage, salt damage and drought) are analyzed by using a fluorescent quantitative PCR technology, in such a way, people find that the cold resistance, drought resistance and salt resistance of a transgenic tobacco plant can be improved through the overexpression of the gene in tobacco; the gene can be used for genetically transforming other monocotyledons and dicotyledons and improving the stress resistance of the monocotyledons and the dicotyledons.

A method of plant micopropagation is provided whereby a thin section of non-callus tissue obtained from a monocotyledonous plant, such as sugarcane, wheat or sorghum, is cultured in the presence of a cytokinin and / or an auxin. Optimal regeneration occurs when a basal surface of the thin section is oriented so as to be substantially not in contact with the culture medium. This micropropagation method produces mature monocotyledonous plants by either organogenic or embryogenic regeneration without substantial callus formation. By avoiding use of callus as a starting tissue, this method reduces the likelihood of propagated plants displaying somaclonal variation.

The invention relates to a monocotyledon transgenic method for invading growing points of seed buds minimally and fully. According to the technical key point, the monocotyledon transgenic method comprises the following steps of: germinating seeds for 1 to 2 days, and when buds extend to be 0.2 to 2 centimeters long, removing coleoptile to expose the growing points; pricking and brushing the growing points by using a minimally-invasive brush of which the diameter of a single brush hair is 4 to 20 micrometers, the exposed length is 0.5 to 3 millimeters and the number of the brush hairs is 100 to 5,000 and which is dipped with agrobacterium mediated conversion liquid to perform full minimally-invasive conversion; after performing co-culture, developing further to form seedlings, promoting the development of spikes and grains, and harvesting in a plant division mode; and performing identification on T1 generation. The monocotyledon transgenic method has the advantages that tissue culture, the limit of genotypes and resistance screening are avoided, the method is easy and convenient to operate and easy to realize in large scale and is suitable for all monocotyledons which bear seeds. By the monocotyledon transgenic method, the transformation effects that the genetic transformation rate of wheat is 49 percent, the genetic transformation rate of paddy is 66.3 percent and the genetic transformation rate of corn is 100 percent are achieved.

The disclosure describes a transgenic dicot or monocot plant having bovine milk protein(s) and methods of producing the transgenic dicot or monocot plant containing bovine milk protein(s). These transgenic dicot or monocot plants can express and produce bovine milk protein(s). The methods involve introducing a recombinant DNA construct expressing a bovine milk protein into a dicot or monocot plant, obtaining the dicot or monocot plant containing the bovine milk protein(s) from a recombinant DNA construct, cultivating and harvesting the transgenic dicot or monocot plant, and extracting and purifying the bovine milk protein(s) from transgenic dicot or monocotyledonous plants.

The invention discloses an artificial synthesized Bt insecticidal gene for transgenic anti-insect plants, further, the invention relates to the optimization and transformation systematically to a coding frame and a codon of a CrylAc anti-insect gene, according to an activity analysis to Bt protein in different regions and in accordance with the coding characteristics of the monocotyledons, obtaining anti-insect gene CrylAc-M which can be expressed in a monocotyledons efficiently, with the optimized codon and coding frame. The CrylAc-M gene after being transformed is connected on an expression carrier with CaMV35S-Adhl as promoter, and then the Bt gene CrylAc-Adhl is transferred into a maize inbred line with highly efficient conversion rate by a gene gun co-transformation method. A transgenic progeny is positive through Bt protein testing and a field anti-insect test of the field is tested to be highly resistant.

The invention discloses a soybean zinc finger protein gene SCTF-1 which is a C2H2-type zinc finger protein gene which is discovered in soybean and is relevant with low temperature. The experiment shows that the protein coded by SCTF-1 can be located to a cell nucleus; after being excessively expressed, the gene can obviously improve cold resistance of transgenic plants, and has a function of improving plant comprehensive stress tolerance. The gene provided by the invention is derived from soybean, and comprises an excellent codon of dicotyledon, such as soybean and the like; and the gene engineering receptor of the gene is mainly suitable for dicotyledon, such as soybean, tobacco and cotton and the like, and also suitable for monocotyledon, such as rice, wheat and corn and the like.

The invention discloses N-pyridine aryloxyphenoxy carboxylic acid derivatives represented by the formula (I), a preparation method and applications thereof. In the formula (I), the definitions of Ar1, Ar2, R, R', R1, R2, R3, R4, m, n, p, q, and X are represented in the description. The compounds (I) has activities on killing weeds, bacteria, and insects, and has extremely high activity on killing monocotyledon weeds such as Chinese sprangletop.

The invention belongs to the technical field of biology, and particularly discloses a gene editing vector system based on barley stripe mosaic virus. The gene editing vector system comprises artificial plasmids respectively containing RNA[alpha], RNA[beta] and RNA[gamma] of the barley stripe mosaic viruses; and in the RNA[beta] or the RNA[gamma], a desired sgRNA sequence is integrated. The gene editing vector system based on the barley stripe mosaic virus can perform efficient gene editing on genomes of dicotyledons such as nicotiana benthamiana and monocotyledons such as wheat and corn.