Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying hybrid germplasm of actinidia based on genome heterozygosity

A kiwifruit and heterozygosity technology, applied in the field of plant genetic resource evaluation and biology, can solve problems such as bias, incomplete marker coverage, errors, etc., and achieve the effect of saving time and reducing unit sequencing cost.

Inactive Publication Date: 2015-05-20
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitations of the markers used in traditional heterozygosity assessment, the comparison of interspecies results is unreliable, especially when hybrid introgression only produces genetic exchange in a part of the genome region of the species, and the incomplete coverage of markers can lead to bias and even errors. the result of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying hybrid germplasm of actinidia based on genome heterozygosity
  • Method for identifying hybrid germplasm of actinidia based on genome heterozygosity
  • Method for identifying hybrid germplasm of actinidia based on genome heterozygosity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0027] 1. Method

[0028] 1. Step ①

[0029] Such as figure 1 , Genome low-depth sequencing-1

[0030] Selection of ancestral basic species of kiwifruit and sequencing strategies suitable for hybrid germplasm identification.

[0031] A. Screening 10 basic ancestral species of kiwifruit

[0032] According to the existing Actinidia species group, these basic species evolved independently, representing more than 90% of the morphological and geographical distribution variation of the plants of the genus, while the genome was basically not eroded by exogenous genetic material;

[0033] B. Perform 5-fold genome low-depth sequencing on these 10 ancestral basic species and germplasm to be identified

[0034] According to the genome size of kiwifruit about 700M, the amount of data obtained is about 3.5G, which can meet the identification requirements of this embodiment.

[0035] The specif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying hybrid germplasm of actinidia based on genome heterozygosity, relating to the technical fields of plant genetic resources and biology. The method comprises the following steps: (1) performing low-depth genome sequencing on a sample; (2) comparing reference genomes of sequencing data and mining single-base mutation sites; (3) calculating the heterozygosity of genome level of the sample based on the single-base mutation sites; (4) constructing a basic species heterozygosity distribution rule database of a main ancestor of the actinidia; and (5) performing heterozygosity comparison on a to-be-detected sample and the database so as to identify the hybrid germplasm. Compared with the traditional molecular marker technology, the method disclosed by the invention has the advantages that the identification accuracy is greatly improved; and meanwhile, compared with the traditional morphological identification method, the method disclosed by the invention is high-efficiency and rapid, and the identification time and cost can be saved. The method disclosed by the invention has wide applicability on different sequencing platforms, and comprehensive evaluation and application of the germplasm resources of actinidia can be formed.

Description

technical field [0001] The invention relates to the fields of plant genetic resource evaluation and biotechnology, in particular to a method for identifying kiwifruit hybrid germplasm based on genome heterozygosity. It specifically involves low-depth genome sequencing of Actinidia plant germplasm and genome heterozygosity calculation based on sequencing data, basic database construction of genome heterozygosity of main ancestral species groups, comparative analysis of germplasm to be identified and final hybridization of germplasm identification identification. Background technique [0002] Actinidia ( Actinidia Lindl.) Plants have 54 species and about 75 different taxa. Among the various genetic germplasm resources of kiwifruit collected and preserved in nature and artificially, there are a large number of hybrid germplasm resources due to its botanical characteristics of dioecious perennial. Due to the integration of different genetic resources and genomes, hybridizati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2537/165
Inventor 刘义飞李大卫黄宏文
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com