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112 results about "Zygote formation" patented technology
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In multicellular organisms, the zygote is the earliest developmental stage. In single-celled organisms, the zygote can divide asexually by mitosis to produce identical offspring. Oscar Hertwig and Richard Hertwig made some of the first discoveries on animal zygote formation.
Preparation method for transgenic mice capable of producing human nerve growth factor
ActiveCN104561095AEasy to operateIncrease success rateAnimals/human peptidesVector-based foreign material introductionPlasmid dnaGenetically modified mouse
The invention discloses a method for producing transgenic mice through a homologous recombination technology. The method comprises the following steps: replacing mouse NGF genes on mouse chromosomes by human NGF genes through a Cas-9 / CRISPR gene knock-in technology, and obtaining the genes with homozygous human NGF genes through breeding and knocking the genes in mice, thus obtaining filial generation mice with salivary glands capable of secreting human NGF. Compared with the conventional targeting technology utilizing positive and negative double screening homologous recombination genes, the method disclosed by the invention is simple to operate, capable of being realized only by transfecting mouse embryonic stem cells by three plasmid DNAs or carrying out pronucleus injection on the zygotes of mice, and high in success rate which is up to 2-5% and remarkably higher than the mouse embryonic stem cell positive rate of 0.1% of the common gene targeting technology.
Owner:深圳市国创纳米抗体技术有限公司
Ecological breeding method for sea horses
InactiveCN101589692AImprove the growing environmentImprove farming outputClimate change adaptationPisciculture and aquariaSeawaterZygote formation
The invention relates to an ecological breeding method for sea horses, which is characterized by comprising the following steps: putting sea water in which zygotes of fishes, shrimps and shellfishes are filtered into a cement pool, ensuring that the height of the sea water is 0.2 to 0.4 meter lower than that of the cement pool, and keeping the temperature of the sea water between 15 and 32 DEG C and the salinity of the sea water between 1 to 3 percent; putting kelps into the cement pool, and keeping the fresh weight density of the kelps in the cement pool between 0.5 and 2 kg / m; inoculating organic fertilizer to the cement pool to breed bioactive baits; putting the sea horses to the cement pool; and during breeding, keeping the density of the living biological baits in the cement pool. Compared with the prior art, by adopting the method for breeding the sea horses, little bait even no bait is fed, so the cost is low and the pollution is little; and the produced sea horses have high survival rate, large individual and good economic benefit; and the method can be used for supplementing the resources of coastal sea horses, and broadens the path of marine organism resource protection.
Owner:NINGBO UNIV
Nuclear transfer with porcine embryonic stem cells
Provided is a method for the production of transgenic animals, especially pigs, by the use of nuclear transfer from genetically modified or other embryonic stem cells to either enucleated oocytes which were matured in vivo or in vitro and activated or to enucleated zygotes.
Owner:BOARD OF TRUSTEES OF THE UNIV OF ILLINOI THE
Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method
ActiveCN1922990AGonadal synchronizationStrong stress resistanceAnimal reproductionClimate change adaptationZoologyOedogonium
The invention relates to a method for crossing and breeding red Po and john dory. Wherein, it is characterized in that: in winter and spring, selecting adult and healthy wide ones as parent; breeding the parent; at later may and middle June, ejecting odinagogues HCG and LRH-A2 into parents; and artificially seminating while the ratio between female and male is 1:3, and cultivating the zygote in cultivation cylinder or channel. The invention has low cost and easy transportation.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES
Methods for in vitro fertilization
InactiveUS6838235B2Improve developmentSpeed up the processNew breed animal cellsCulture processPlant Germ CellsReproductive tract
Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.
Owner:VITROLIFE AB
Telegenesis and oosperm hatching technique for whitebait
ActiveCN101218899AIncrease productionQuality improvementClimate change adaptationPisciculture and aquariaWater qualitySemen
The invention relates to the technical field of fish propagating, in particular to a technique of artificial insemination and hatching zygote for whitebait and is provided with 1. artificial insemination process: extruding semen and an egg granule in a container, adding normal saline of 0.004 to 0.008 percent, stirring and standing for 2 to 3 minutes; 2. fertilized egg hatching process and prophase hatching: the fertilized eggs are arranged in a hatching chamber and is poured into a hatching tray after sterilized by the normal saline of 0.004 to 0.008 percent and hatched by adding purifying water; the temperature of the hatching chamber is 16 to 20 DEG C, avoiding air, lighting and avoiding from being directly irradiated by sunshine and changing water as well as controlling the water temperature; anaphase management: after the fertilized eggs begin to emerge, stopping changing water and sterilizing, adding purifying water and arranging the fries beginning to swim flatly into a culture pool after 48 to 72 hours. The technique of the invention adopts a reasonable and scientific method and has the processes of sterilizing, water quality control, temperature control, appliance control and environment control, etc., which greatly improves the yield and quality of the fries.
Owner:江苏太滆水产科技有限公司
Artificial propagation method for yellowfin groupers
ActiveCN110100768AImprove environmental adaptabilityIncrease food intakeClimate change adaptationAnimal feeding stuffBroodstockRed mullet
The invention provides an artificial propagation method for yellowfin groupers. The artificial propagation method comprises the steps that (1) parents are selected; (2) a parent breeding pond is separated into a deep water area and a shallow water area with water flow communicated, wherein the water depth of the deep water area ranges from 4 m to 5.5 m, and the water depth of the shallow water area ranges from 3 m to 3.5 m; (3) parent fish breeding management is carried out, wherein a, in first 3 weeks during breeding, LED green light and yellow light alternately irradiate the water surface ofthe pond, and after the parent fishes are bred for 3 weeks or longer, the water surface of the pond is irradiated by the LED green light; b, feed is prepared from 3-5 parts of grouper compound feed and 1-2 parts of small fish and shrimp and artificial pellet feed, the parent fishes are fed with the first feed in the first 3 weeks during breeding, and the parent fishes are fed with the second feedafter being bred for 3 weeks or longer; c, the water temperature of the pond ranges from 21 DEG C to 26 DEG C, and water dissolved oxygen is larger than 4.8 mg / L; (4) breeding is enhanced before propagation is carried out; (5) artificial propagation is carried out; (6) zygotes are collected and hatched. According to the artificial propagation method for the yellowfin groupers, the adaptive capacity to environment of the parent fishes is enhanced, the activities of fish shoals are effectively promoted, the feeding rate is effectively increased, the fertility is high, and large-scale artificialbreeding of the yellowfin groupers can be effectively achieved.
Owner:HAINAN CHENHAI AQUATIC CO LTD
Immunofluorescence dyeing viewing method for left-eyed flounder zygote microtubule skeleton
InactiveCN1955738AEasy to observeEasy to storeBioreactor/fermenter combinationsBiological substance pretreatmentsLaser scanningEmbryo
An immune fluorescent microscopic observing method of cytula micro-pipe skeleton from bothidae flasfish includes applying fixture solution to fix sample and utilizing dissection needle to separate germ disc from embryon, making hole on sample and using sodium borohydride to eliminate aldehyde group influence on experimental result, carrying out indirect immune fluorescent staining on sample then using fluorescence microscope to observe sample.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Seedling breeding method for dollar spot Scophthalmusmaximus
InactiveCN1922991AImprove survival rateArtificial breeding technology is scientific and reasonableAnimal reproductionClimate change adaptationShrimpSemen
The invention relates to a method for breeding sole, which comprises: (1) cultivating parents that 1, adjusting water temperature that naturally decreasing 6-7Deg. C, and increasing to 12Deg. C; 2, adjusting light with lamp; 3, adjusting bait that uses shrimp, fish, etc, with vitamin and lecithin; 4, daily management that clearing and exchanging water; 5, using HCG and LHRH to accelerating growth; (2), picking up semen and eggs, and cultivating zygote, while the zygote is obtained by dry semination, and using sinke zygote and semi-sink zygote to cultivate the baby fish; (3), breeding seed that cultivating 3-5cm seeds in small and large pools, while the total survival rate can reach 28.0-30.0%, and the total survival rate at 8-9cm can reach 21%. The invention can improve the survival rate of seeds.
Owner:大连天正实业有限公司
WZ(male)-ZZ(female) type Oreochromis aureus ZZ zygote feminization method
InactiveCN101416613AImprove conversion rateIncrease contentClimate change adaptationPisciculture and aquariaHormones regulationBiology
The invention relates to a feminization method for ZZ zygocyte of Oria tilapia of which the male parent is ZZ and the female parent is WZ, belonging to the field of tilapia cultivation. The technical proposal is as follows: before the gonad of the Oria tilapia starts differentiation, feeding the Oria tilapia with tonic that contains estrogen hormone for more than 15 days, and then using the ordinary way of feeding and management to get ZZ type physiologically female fish, wherein every gramme of tonic contains 100 to 200 microgrammes of estrogen hormone, and the temperature for feeding with the tonic is 25 to 30 DEG C. The invention can change the hereditarily male fish into physiologically female fish, such that it sets up a novel breeding system of ZZ type all male Oria tilapia having significant meaning in the expanding and breeding of all male Oria tilapia.
Owner:广州鹭业水产有限公司
Method for collecting and separating medaka zygotes
The invention discloses a method for collecting and separating medaka zygotes, and relates to the technology of fish zygote separation. In order to solve the technical problems, the invention provides the method for collecting and separating the medaka zygotes for preventing egg membranes from being punctured. The invention adopts the technical scheme that the method adopts a soft small brail, disposable engineering plastic inoculation cups, a large-mouth suction tube and culture dishes. The method is characterized by comprising the following steps of: fishing female fishes of which bellies are hung with eggs within 1 to 6 hours after laying eggs to the water surface by using the soft small brail, taking down the egg blocks by using the disposable inoculation cup, and transferring the taken-down egg blocks to the glass culture dish filled with Ringer's culture solution to perform separation; and mutually stretching the inoculation cups held in the left and right hands to break the egg silks and separate the zygotes, sucking the separated single eggs to the other culture dish filled with the Ringer's culture solution by using the large-mouth suction tube to perform hatch, and controlling the hatching temperature to be between 25 and 26 DEG C. The method is used for embryo observation and experiments.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
In-vitro hatching method for freshwater lobster zygote
InactiveCN101743924AIncubation takes up less landReduce feeding and management costsClimate change adaptationPisciculture and aquariaWater useFresh water organism
The invention discloses an in-vitro hatching method for freshwater lobster zygote. The technical scheme comprises the steps of: putting oogenesis parent lobsters fished in batch into a methylene blue solution to be soaked for 5-15 minutes; lightly stripping zygotes from parent lobster appendages by using sterile tweezers, and putting into a zygote hatcher; and hatching into post larvae after 16-26 days, wherein the hatching rate of the zygote can achieve more than 95%. The method has the advantages of large zygote amount, little occupation land for zygote in-vitro hatching, little water using amount, labors and materials saving, no need of continuous breeding of the oogenesis lobster, and reduction of feeding management cost of the oogenesis lobster. The embryo can survive, and quickly hatched into larvae by adopting the in-vitro hatching method; the size of the larvae is uniform, and the commercial value is high; the mutual killing phenomenon among the larvae can be effectively prevented, and the survival rate of larvae is enhanced; and the in-vitro hatching method is suitable for the in-vitro hatching of scaled freshwater lobster zygotes.
Owner:JIANGSU XUYI RIVERRED CRAWFISH ECO PARK
Device and method for incubating zygotes of fishes
ActiveCN105613387AEnhance flushing habitsSatisfy the flushing habitClimate change adaptationPisciculture and aquariaFisheryWater flow
The invention belongs to the field of fish breeding, and mainly relates to a device for incubating zygotes of fishes. The device comprises an incubating pond. A first water inlet is formed in the bottom of the incubating pond, a second water inlet is formed in a position above the incubating pond, and artificial fish nests for collecting roes are arranged in the incubating pond. A shower head for spraying water into the incubating pond is connected to the second water inlet. The device has the advantages that water is supplied into the incubating pond from bottom to top by the first water inlet in the bottom of the incubating pond, so that water in the incubating pond can be always in a flowing state, sufficient oxygen in the water can be guaranteed, oxygen deficient conditions of the roes due to the fact that the roes cohere with one another can be prevented, and the roes can be assuredly normally incubated; the water can be sprayed on a water surface of the incubating pond from top to bottom by the second water inlet, impact force of the water can be relieved by a shower head structure under the condition that requirements on water changing quantities are met and water flow is guaranteed, and accordingly the zygotes and / or fish fry just out of membranes can be prevented from being burst open by the water flow.
Owner:ANHUI AGRICULTURAL UNIVERSITY
Boot method, boot device and Android intelligent device
The invention provides a boot method, a boot device and an Android intelligent device. The method comprises the following steps of starting ActivityManagerService in a SystemServer process created by a Zygote process in a boot process; sending custom BOOT-P0 broadcast for starting a system service or a system application with the first priority in a FinishBooting function or other processes by the ActivityManagerService; after the custom BOOT-P0 broadcast is sent for the preset time, sending custom BOOT-P1 broadcast to start a system service or an application whose boot priority is next only to the system service or application started by the BOOT-P0 broadcast; and after the custom BOOT-P1 broadcast is sent for the preset time, sending original ACTION-BOOT-COMPLETED broadcast for starting a third-party application and / or a system service with third priority. The custom BOOT-P broadcast can be increased according to the number of the system services which need to be started, so that the applications or the services to be started are started in a boot process according to the order of the priority, and thus the system lag caused by CPU occupation due to centralized startup of many applications or services at the same moment is avoided, the system burden is relieved and the smoothness of system running is improved.
Owner:SHENZHEN TCL DIGITAL TECH CO LTD
Method for collecting seedlings from scytosiphon filaments
InactiveCN101946683ASolve the limitation of algae breeding seasonResolution timeClimate change adaptationCultivating equipmentsSporangiumBiology
Owner:DALIAN OCEAN UNIV
Tilapia male and female pairing propagating method
InactiveCN101138329ASolve the problem of germplasm degradationControl inbreedingClimate change adaptationPisciculture and aquariaGerm plasmTilapia
The present invention relates to a male and female mating propagation method of tilapia, in specific to a mating propagation of tilapia which can adopt the pattern of male mating female; the method belongs to the technical field of aquatic product breeding. The method is characterized in that a net cage is equipped in an outdoor pond or indoor concrete pond; adult male tilapias are selected and people cut the upper jaw of the tilapias and put the tilapias into the net cage after being sterilized; and then adult female tilapias are selected to put in the net cage for mating culture and people can see the zygotes in the mouths of female tilapias after 7 to 10 days. With the present invention, a tilapia family line can be established for the family breeding of tilapias. In addition, people can clearly know the relation between the offspring and parent to control the inbreeding and avoid the germ plasm degeneration of offspring.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Cynoglossus semilaevis ovum miosis gynogenesis method induced by bass frozen sperm
InactiveCN101011037AGuaranteed reliabilityIncrease flexibilityAnimal reproductionClimate change adaptationWater bathsSebastes
The invention relates to a method for using the frozen sperm of bass to induce the cultivation of sliding-tongue ovum thelykaryon, which comprises that: using the bass sperm frozen in the liquid nitrogen, to induce the thelykaryon cultivation of sliding-tongue ovum; defreezing the sperm in 37Deg.C water bath, taking 100muL sperm to dilute the MPRS for 10 times, to be arranged in the cultivate container at 6Deg. C; using 1000muj / cm2 ultraviolet to radiate for 0.2min, to inactivate the sperm; then using the inactive sperm to induce the fecundation with the ovum; in 5min of fecundation, using 2Deg. C water bath to shock the zygote, while the shock time is 20min; arranging the blast into the sea bath at 23Deg. C to be cultivated. The invention has low cost.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Method for fluorescent double-labeling microscopic observation of marine flounder oosperm microtubules and cell nucleuses
InactiveCN103364570AAvoid influenceFacilitate research and explore dynamic changesBiological testingMicroscopic observationLaser scanning
The invention discloses a method for fluorescent double-labeling microscopic observation of marine flounder oosperm microtubules and cell nucleuses. The method comprises the following steps of carrying out fixing of an oosperm with a membrane, stripping a blastoderm of the fixed oosperm from a whole embryo by a dissecting needle, punching the stripped blastoderm, reducing or eliminating the influence produced by uncombined aldehyde groups on an experimental result by sodium borohydride, carrying out sample sealing and fluorescent double-labeling dyeing, and carrying out sample observation by a fluorescence microscope or a laser scanning confocal microscope. The method has low sample requirements, has simple processes, is convenient for observation, produces a clear result, has a high three-dimensional feel, can be used for dynamic change research on flounder oosperm microtubules and cell nucleuses from blastoderm formation respectively to 2-cell, 4-cell and 8-cell development stages, is suitable for other larger telolecithal eggs of marine flounder oosperms, and has a wide application range.
Owner:QINGDAO AGRI UNIV
High efficiency, high throughput generation of genetically modified mammals by electroporation
InactiveUS20170298390A1Genetically modified cellsMammal material medical ingredientsMammalElectroporation
The invention described herein provides high throughput methods and reagents for generating transgenic animals (e.g., mammals) through introducing materials into gametes or preimplantation stage (e.g., one-cell embryo or zygotes) via electroporation, leading to genetically inheritable modification to the genome of the animal.
Owner:JACKSON LAB THE
Fluorescent strain E. coli C600 and construction method and application thereof
ActiveCN110066820AImprove stabilityGenetic stabilityBacteriaMicroorganism based processesTransfer geneMixed culture
The invention provides a fluorescent strain E. coli C600 and a construction method and application thereof. By constructing a recombinant plasmid containing an R6k replicon, a transposition unit and an RP4 conjugative transfer gene, the recombinant plasmid is transferred to a host bacterium in which the recombinant plasmid can replicate to obtain a recombinant strain, the recombinant strain and E.coli C600 are subjected to mixed culture, and then the fluorescent strain E. coli C600 can be obtained through screening, wherein the transposition unit is formed by using two ISApl1 for sandwichinga Lux gene cluster and a tellurite drug-resistance gene in the middle. The exogenous gene acquiring capability of the fluorescent strain E. coli C600 is not influenced, the stability is high, stable inheritance can be ensured, the fluorescent property cannot lose along with bacterium passage, and thus the fluorescent strain E. coli C600 can serve as a recipient bacterium for conjugation experiments. By adopting the fluorescent strain E. coli C600, recipient bacteria and zygotes can be identified more efficiently and more intuitively in the fields related to plasmid conjugational transfer, andtherefore the fluorescent strain E. coli C600 has good application prospects.
Owner:SOUTH CHINA AGRI UNIV
Seashell spawning method using chemo-induction
InactiveCN101331861BPromote maturationPromote maturityClimate change adaptationPisciculture and aquariaVitamin COospore
Owner:山东安源种业科技有限公司
Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy
Owner:SYNAGEVA BIOPHARMA CORP
Kit and method for constructing ApoC2 gene knockout hamster model
ActiveCN109234278ANot easy to missImprove efficiencyHydrolasesStable introduction of DNAHamsterMicroinjection
The invention discloses a kit and a method for constructing an apolipoprotein C2 (ApoC2) gene knockout hamster model, wherein the kit comprises sgRNA, which is obtained by PCR amplification of an artificial sequence as shown in SEQ ID NO: 2 and SEQ ID NO: 3, and in vitro transcription. The method comprises the following steps: designing the hamster ApoC2 gene specific targeting sequence, and preparing the cas9 mRNA and the sgRNA; Collection and culture of hamster fertilized eggs; Microinjection: co-injecting the sgRNA and cas9 mRNA into the cytoplasm of a hamster fertilized egg; Finally, the fertilized eggs were transplanted into the surrogate hamsters and the F0 generation hamsters were born. The ApoC2 gene knockout hamster model was successfully constructed by this method.
Owner:北京华阜康生物科技股份有限公司
Daiquzu pseudosciaena crocea and minyuedongzu cultured pseudosciaena crocea cross breeding method
InactiveCN1951183AOvercoming differences in the timing of individual sexual maturityOvercome spatial differencesAnimal reproductionClimate change adaptationFrozen storageRelationship - Father
The invention relates to a cross method between large yellow Crocker and GuangDong large yellow Crocker. Wherein, their crossed generation has high grow speed and the application in long transmission; the invention selects GuangDong large yellow crocker as father, whose belly can be pressed to flow sperm; preparing dilute solution; compressing its belly to absorb sperm, whose activity is higher than 90% to be frozen and stored; mixing the dilute solution and anti-freeze agent, adding sperm, storing in frozen storage tube, sealing and putting in 4Deg. C; arranging it above the liquid nitrogen, reducing temperature and moving it into liquid nitrogen; selecteing the wide GuangDong large yellow crocker as mother; when its ovogen is mature, defreezing the sperm, and taking the freeze storage tube from liquid nitrogen to be put into room-temperature fresh water to be defrozen, activating it with seawater; artificial inseminating and washing zygote with seawater, removing sink zygote, and charging air and cultivating.
Owner:XIAMEN UNIV
Sturgeon zygote incubation device
InactiveCN105123579AAvoid deathAvoid harmClimate change adaptationPisciculture and aquariaWater flowSturgeon
Owner:CHINESE STURGEON RES INST CHINA THREE GOR
Artificial breeding method of new species of Murray cods
The invention relates to an artificial breeding method of a new species of Murray cods. Conditions for culturing the new species in the source area Australia are simulated. The method includes the following steps that a temperature-controlled parent Murray cod breeding pond is built, a parent Murray cod nest is arranged, parent Murray cod breeding is carried out, parturition is hastened, artificial impregnation is carried out, zygotes are artificially hatched, and Murray cod fries are cultured; in this way, artificial breeding of the Murray cods is successful, and the artificial breeding method is an innovation in China.
Owner:JIANGSU ZHONGYANG GRP
Anhydrous transportation method for medaka zygotes
InactiveCN101828545AAvoid risk of deathFacilitate communicationDead animal preservationNormal growthBiology
The invention discloses an anhydrous transportation method for medaka zygotes, which relates to the technology of fish zygote transportation. In order to solve the technical problems, the invention provides the transportation method capable of keeping appropriate humidity and dissolved oxygen at the normal temperature so as not to affect the normal growth of the zygotes. The invention adopts the technical scheme that the method comprises the following steps of: selecting the zygotes of which fertilization time is 2 to 6 hours, removing whitish opaque dead eggs, flushing the zygotes by using Ringer's culture solution and then draining the water, filling the zygotes into a 2-5mL centrifuge tube provided with a plastic flat bottom and a cover and filled with solid oxygen beads to ensure thatthe total volume of the zygotes is about 4 / 5 of that of the centrifuge tube, covering the cover and then sealing the centrifuge tube by using a sealing tape, filling the sealed centrifuge tube into an A4-sized kraft envelope bag, and then putting the envelope bag into an EMS envelope to perform sealed transportation. The method is suitable for quickly transporting the medaka zygotes in an expressmode.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Method for inducing ocean seashell polyploidy by using sodium chloride
InactiveCN101658149AInhibition releaseEasy to operateClimate change adaptationPisciculture and aquariaPolar bodyMicroscopic exam
The invention discloses a method for inducing ocean seashell polyploidy by using sodium chloride. The method is characterized by comprising the following steps: selecting the seashell to grow the shell to respectively obtain sperms and ovum; carrying out artificial insemination; continuously observing the developing condition of zygotes through microscopic examination to determine the number of the water body for culturing the zygotes; adding sodium chloride into the culture sea water containing the zygotes according to a proportion that 20-40 go of sodium chloride is added in 1000ml of culture seat water when 40-50 percent of zygotes are observed to release a first polar body or a first zygote is observed to occur the first polar body, so as to prevent the release of a second polar body or the first polar body of the zygotes; evenly stirring and treating for 10-15 min; and transferring the zygotes to normal sea water to incubate . The ocean seashell comprises Pacific oyster, chlamys farreri, Argopecten irradians, Crassostrea rivularis, clam, Scapharca broughtonii and the like. The seashell zygotes are treated by using the sodium chloride and the initiating agent so as to inhibit the release of the polar body and produce the shell polyploidy with no toxicity, efficiency, simple operation and extremely low cost.
Owner:山东安源种业科技有限公司
Sperm-specific monoclonal antibody, mAbC
InactiveUS7053187B2Mammal material medical ingredientsImmunoglobulins against cell receptors/antigens/surface-determinantsGene deliveryHuman body
A method and vector system for delivering of a gene into a human stem cell for therapeutic uses is disclosed. The method includes linking a therapeutic gene to human sperm cells through a linker and fertilizing a human oocyte. The resulting zygote may then be cultured and established as human embryonic stem cells, which may later be differentiated into different types of cells for transplantation into the human body.
Owner:KWANG HUA DEV & INVESTMENT
Mutants of fatty acid adenosine monophosphate (AMP) ligase with improved substrate affinities and application thereof
The invention relates to mutants of a fatty acid adenosine monophosphate (AMP) ligase and application of the mutants, and belongs to the field of bioengineering. The fatty acid AMP ligase comes from streptomyces roseosporus. The 296th glycine of the fatty acid AMP ligase is changed into alanine, and the 302th isoleucine of the fatty acid AMP ligase is changed into valine to respectively obtain the mutants pAL296 and pAL302. The mutants of the fatty acid AMP ligase can improve the binding level with exogenous precursor decanoic acid to enhance the yield of daptomycin. According to the invention, an encoding gene dptE of the fatty acid AMP ligase is subjected to site-directed mutagenesis in vitro by using computer simulation, overlap extension PCR and site-directed mutagenesis technologies to screen out the mutants pAL296 and pAL302 of which the affinities are obviously improved, recombinant shuttle plasmids pAK296 and pAK302 are constructed and sequentially led to the streptomyces roseosporus, and zygotes are screened to obtain streptomyces roseosporus DCE296 and DCE302 of the fatty acid AMP ligase with improved substrate affinities to lay a foundation for improving the production level of daptomycin.
Owner:CHINA PHARM UNIV
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