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Pediococcus pentosaceus G11 strain as well as screening and applications thereof

A technology of Pediococcus pentosaceus and bacterial powder, applied in the directions of application, bacteria, antibacterial drugs, etc., can solve the problems of insignificant effect and the like, and achieve the effects of reducing animal mortality, improving immunity, and having wide application prospects.

Active Publication Date: 2016-12-07
SHANTOU UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Probiotics have been applied to prawns, shellfish and other marine aquaculture animals, but the probiotics suitable for shrimp and shellfish do not necessarily work or the effect is not obvious in mud crab culture

Method used

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  • Pediococcus pentosaceus G11 strain as well as screening and applications thereof
  • Pediococcus pentosaceus G11 strain as well as screening and applications thereof
  • Pediococcus pentosaceus G11 strain as well as screening and applications thereof

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Experimental program
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Effect test

Embodiment

[0031] 1. Isolation, screening and identification of strains

[0032] The strain was isolated from the water sample of the culture pond (the blue crabs in the culture pond grew well and no disease occurred). Gradiently dilute the MRS plate, select a single colony on the MRS medium, shake the bacterial solution, and use the Oxford cup method to detect the strain with the best antibacterial effect on Vibrio parahaemolyticus, re-plate it, separate and purify the strain.

[0033] In order to identify the strains, the scanning electron microscope was used to observe the cell morphology and test some physiological and biochemical reactions of the strains. The scanning electron microscope was as follows: figure 1 As shown, the strain was preliminarily determined to be Pediococcus pentosaceus.

[0034] The primers used for further 16S rDNA sequence analysis were general primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACCTTGTTACGACTT-3') for PCR, cloning and sequencing. The B...

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Abstract

The invention relates to a pediococcus pentosaceus G11 strain as well as screening and applications of the pediococcus pentosaceus G11 strain. The pediococcus pentosaceus G11 strain is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC NO:2016248. The pediococcus pentosaceus G11 strain has tolerance to compound sulfamethoxazole and ceftazidime, has antibacterial activity for vibrio parahaemolyticus, aeromonas hydrophila, vibrio alginolyticus, staphylococcus aureus and beta streptococcus, has certain stress resistance and enzyme producing ability, is free of hemocyte solubility, and has no drug tolerance for most antibiotics; the dipping bath experiment proves that the pediococcus pentosaceus G11 strain is harmless to the host, can improve the immunity of the body, has wide application prospects, can be applied to probiotic preparations, and also can be prepared into bacterial powder which is then added into a feed as a feed additive, further, the growth of pathogenic bacteria in the bodies of the animals is inhibited, so that the death rate of animals is reduced, and the pediococcus pentosaceus G11 strain is a probiotic brain worthy of development.

Description

technical field [0001] The present invention relates to a strain of Pediococcus pentosaceae, in particular to a strain of Pediococcus pentosaceae G11 which has probiotic activity on Scylla pseudosyringa and its screening and application. Background technique [0002] Scylla paramamosain, referred to as Scylla for short, is a wide-salt and wide-temperature marine economic crustacean. It is mainly distributed in the southeastern coastal areas of my country. It is the main mariculture species in eastern Guangdong, especially Shantou. It has fast growth, individual Large, adaptable and disease-resistant, resistant to dry dew, easy to transport, delicious and unique meat, rich in nutrition and other characteristics, it is favored by domestic and foreign producers and consumers. In recent years, blue crab diseases, especially viral diseases (such as blue crab baculovirus disease, reovirus disease), bacterial venereal diseases and parasitic diseases have caused serious economic loss...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A23K50/80A23K10/18A61K35/741A61P31/04C12R1/01
CPCA61K35/741C12N1/02C12N1/205C12R2001/01
Inventor 李升康吕永玲李忠贞刘文华胡忠温小波
Owner SHANTOU UNIV
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