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A kind of fungicide composition and its application in the separation of anthracnose bacteria

A fungicide composition, technology of anthrax bacteria, applied in the direction of fungicide, application, biocide, etc., can solve the problems of low separation rate and difficult separation of target bacteria, and achieve the effects of fast speed, simple preparation, practicality, quickness and cost

Active Publication Date: 2016-08-31
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the surface disinfection time is too long, the target bacteria will be killed as well as the non-target bacteria, resulting in a low isolation rate of the target bacteria or difficulty in separating the target bacteria.

Method used

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  • A kind of fungicide composition and its application in the separation of anthracnose bacteria
  • A kind of fungicide composition and its application in the separation of anthracnose bacteria
  • A kind of fungicide composition and its application in the separation of anthracnose bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 7

[0045] Embodiment 7 contains the culture medium of bactericide

[0046]Weigh 200g of potatoes, peel and cut into pieces, heat in 1L of distilled water for 30 minutes, filter and collect the filtrate with four layers of gauze, then dilute to 1L again, add 14g of agar powder and 18g of glucose to boil, and sterilize with damp heat at 121°C for 20 minutes. That is, the PDA medium is obtained. Add boscalid, cyanoxystrobin, rifampicin and penicillin in the mass ratio mentioned in Example 1 to the PDA medium with a mass ratio of 10:5:50:50, that is, the final concentration of the four agents in the medium Concentrations were 10 μg / mL, 5 μg / mL, 5 μg / mL and 50 μg / mL, respectively, and finally a culture medium containing fungicides was obtained.

Embodiment 8-12

[0047] Embodiment 8-12 contains the culture medium of bactericide

[0048] Same as Example 7, the difference is that the fungicides are the fungicides prepared in Examples 2-6 respectively.

Embodiment 13

[0049] Embodiment 13 three kinds of fungal inhibitors and five kinds of antibiotics are to the inhibition of anthracnose bacteria

[0050] 1. Inhibitory effects of two fungal inhibitors on anthracnose, early blight and fusarium

[0051] Using dimethylformamide as the solvent of boscalid and cyprostrobin, prepare 1ml of boscalid drug mother solution of 50000μg / mL and 1ml of cyprostrobin drug mother solution of 10000μg / mL respectively. The amounts were 52.63mg and 10.71mg in sequence; the two mother solutions were respectively prepared with a serial concentration of 50μg / mL, 25μg / mL and 10μg / mL drug-containing PDA plates of boscalid and a series of concentrations of 10μg / mL, 5μg / mL mL and 1 μg / mL of cyprostrobin-containing PDA plate, and the plate with solvent was used as a control; ), early blight and fusarium sensitization to two fungal inhibitors. Pre-cultivate the strains to be tested on a PDA plate at 28°C for 3-4 days, use a puncher with a diameter of 5mm to punch out th...

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Abstract

The invention provides a sterilizing agent composition and an application thereof in colletotrichum orbiculare isolation. The sterilizing agent composition comprises cyprosulfamide, phenamacril, rifampicin and penicillin or comprises cyprosulfamide, phenamacril, neomycin sulfate and penicillin. A culture medium containing the sterilizing agent composition has the characteristics of selectivity, accuracy, practicability, quickness, low cost and simple preparation. The culture medium provided by the invention is used for isolating the colletotrichum orbiculare and is capable of solving the serious pollution problem of each non-target fungus and bacterium in an isolation process of the colletotrichum orbiculare and other plant pathogenic fungi, besides, the culture medium is rapid in speed, high in efficiency and is time saving and labor saving.

Description

technical field [0001] The invention relates to the field of microorganism separation, in particular to a bactericide composition and its application in the separation of anthrax bacteria. Background technique [0002] Anthracnose bacterium (Colletotrichum spp.) is an important pathogenic bacterium, which is widely distributed and has various hosts. It can harm a variety of crops, trees, ornamental plants, etc., and can also cause human diseases. The pathogenic bacteria that cause anthrax include multiple species (Colletotrichum spp.) within the genus Anthracnose, among which C. gloeosporioides, C. acutatum and C. truncatum are It is the dominant species that causes the anthracnose pathogenic bacteria of various crops in many areas of our country. The anthracnose caused by this pathogen is an important disease worldwide, among which pepper anthracnose is an important disease caused by anthracnose bacteria infecting peppers. It has been reported in various pepper growing are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P1/00A01P3/00C12N1/14A01N43/40A01N37/44A01N43/16C12R1/645
Inventor 刘西莉张灿王为镇刁永朝刘利黄中乔方媛
Owner CHINA AGRI UNIV
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