227 results about "Penicillin K" patented technology

The invention discloses a construction method of a penicillin-producing recombined strain of streptomyces virginiae IBL14. The method involves sequence properties of a beta-lactamase gene containing hydrolysis penicillin and gene clusters producing penicillin, and the whole process of a construction method of the penicillin-producing recombined strain constructed on this basis. Related genes are compiled through the penicillin-producing gene and the CRISPR-Cas I-B gene of streptomyces virginiae IBL14, and therefore the aim of increasing the penicillin yield is achieved. A new route and method are provided for increasing the types of biological medicine, improving the production level and improving the product quality.

The invention discloses a method for preparing activated carbon from penicillin or terramycin strain residues. The method comprises the steps of: crushing strain residues, sieving by a 20-meshed sieve, uniformly mixing the strain residues with a solution prepared according to different activation ratios, soaking at a constant temperature for 24 hours, drying in an oven at the temperature of 105-110 DEG C, placing in a temperature resistant sealed container to prevent oxidization and incineration, placing the sealed container in an activating and carbonizing furnace, heating to an activation temperature, activating at the constant temperature for set time, washing the activated strain residues with hydrochloric acid, then washing with water to be neutral, drying at the temperature of 105-110 DEG C for 2-3 hours, and cooling to room temperature to obtain the strain residue activated carbon. According to the invention, prepared activated carbon is high in specific area, rich in micropores and mesopores and excellent in adsorbing performance; and waste terramycin strain residues from a pharmaceutical factory are utilized to prepare activated carbon, thus the resource, reduction and harmless treatment of the waste strain residues are effectively realized, and the social benefit is significant.

The invention relates to a livestock semen freezing diluent as well as a preparation method and application of the livestock semen freezing diluent. The preparation method of the livestock semen freezing diluent comprises the following steps of: adding 2.71 grams of trihydroxymethyl aminomethane, 1,4 grams of citric acids, 1.0 gram of monosaccharides, 10 myriad IU (International Unit) of penicillium, 10 myriad IU of streptomycin and 0.29-6.97 grams of inositol compounds to a right amount of ultrapure water; optionally adding 5-20 milliliters of penetrability protectants; uniformly stirring; regulating a pH value to 6.8-7.2; adding 5-20 milliliters of fresh yolk subjected to inactivation treatment; uniformly mixing, and then fixing the volume to 100 milliliters; centrifugalizing at low temperature for 1 hour; and filtering supernate to prepare the livestock semen freezing diluent. Because the inositol compounds can effectively inhibit the generation of ice crystals, the livestock semen freezing diluent disclosed by the invention reduces the mechanical injury caused by the ice crystals on semens, achieves the survival rate of the defrozen semens at about 75%, the activity more than 50%, the acrosome integrality at about 60% and the plasmalemma integrality at about 50% and achieves the non-return rate more than 70% after artificial insemination; and the livestock semen freezing diluent has the advantages of no immune response generation, purity guarantee, low price, and the like by using the inositol compounds as the ice crystal inhibitors of the semen freezing diluent.

The invention discloses base fluid for diluting semens of equus animals and a preparation method and a use method thereof. The base fluid contains A fluid and skimmed milk. The A fluid contains glucose, raffinose, D-glucopyranose, trisodium citrate, ppotassium citrate and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). The preparation method for the base fluid includes weighing various components of the A fluid into a sterile beaker, adding double-distilled water, dissolving, bringing to volume, filtering, split charging, sterilizing, and sealing to obtain the A fluid, and adding same amount of the skimmed milk into the A fluid to be evenly mixed. Users can also add penicillin and dihydrostreptomycin to the base fluid or add penicillin, dihydrostreptomycin, yolk and glycerol into the base fluid. The base fluid is suitable for semen dilution and normal temperature preservation, low temperature preservation and freezing preservation of the semens of the equus animals. The base fluid for diluting semens of equus animals and the preparation method and the use method of the base fluid have the advantages of being simple to prepare, convenient to use, good in preservation effect of the semens of the equus animals, multi-purpose, time-saving, labor-saving and applicable to mass production and application.

The invention discloses a kit for detecting beta-lactam antibiotic ligand in milk by a receptor method and a detection method thereof. The kit comprises the following components: (1) an enzyme-labeled plate which is coated by penicillin-binding protein PBP2xa or PBP2xb recombinant protein; (2) an enzyme-labeled marker; (3) standard solution of ampicillin sodium; (4) 20 times concentrated cleaning buffer solution; (5) enzyme-labeled diluent; (6) 20 times concentrated sample extract; (7) developing solution; and (8) reaction stop solution. The kit of the invention can simultaneously screen eight beta-lactam antibiotic residues in the milk; the detection limit is lower than the minimum residue limit of China and main developed countries in the world; the detection time is within 50 minutes; and the kit has the characteristics of simple, rapid and accurate operation, low cost and the like and is suitable for large-scale popularization and application.

The present invention describes an assay method comprising: (A) generating (1) at least a first fragment of a reporter molecule linked to a first interacting domain and at least a second fragment of a reporter molecule linked to a second interacting domain, or (2) nucleic acid molecules that code for (A)(1) and subsequently allowing said nucleic acid molecules to produce their coded products; then, (B) allowing interaction of said domains; and (C) detecting reconstituted reporter molecule activity, where said reporter molecule can react with a penicillin- or a cephalosporin-class substrate.

PCT No. PCT / EP96 / 03253 Sec. 371 Date Jan. 15, 1998 Sec. 102(e) Date Jan. 15, 1998 PCT Filed Jul. 16, 1996 PCT Pub. No. WO97 / 04086 PCT Pub. Date Feb. 6, 1997Penicillin G acylase is immobilized by covalent bonding to a crosslinked mixture of a gelled gelling agent such as gelatin and a polymer containing free amino groups such as alginate amine, chitosan or polyethylene imine. The immobilized penicillin G acylase provides a higher synthesis / hydrolysis ratio as compared to immobilizing with other carriers when producing beta -lactam derivatives by a condensing reaction of an amino beta -lactam with an acylating agent. The acylating agent may be a derivative of D-phenylglycine, a derivative of D-p-hydroxyphenylglycine or a derivative of D-2,5-dihydro-phenylglycine. Examples of beta -lactam derivatives that can be produced are amoxycillin, ampicillin, cephaclor, cephadroxil, cephprozil, cephalexin and cephradine.

The invention relates to the technical field of medicines, in particular to a twin antibiotic compound formed by bonding each two parent nucleuses with the same structure of a beta-lactam antibiotic compound or of a derivative of the beta-lactam antibiotic compound with a dicarboxylic acid by two amido bonds, preparation method thereof and use thereof. The chemical structural general formula of the twin antibiotic compound is represented by a formula III. In the formula, R3 is a parent nucleus structure of a molecule of a penicillin compound or a derivative of the penicillin compound or a molecule of a cephalosporin compound or a derivative of the cephalosporin compound; and R may be alkyl and aryl or heteroaryl or substituted alkyl and substituted aryl or heteroaryl. In-vitro antibacterial experiments show that the beta-lactam twin antibiotic compound of the invention has remarkable antibacterial activity and is a novel antibacterial compound. The beta-lactam twin antibiotic compound of the invention can be used in the preparation of bacteriostats or bacteriacides as well as anti-infection medicaments. According to the general knowledge of pharmacy, the compound of the invention can be made into pharmaceutically acceptable salts or hydrates.

The invention discloses a treatment method of penicillin mushroom dregs. The treatment method comprises the following process steps of: (A), pyrohydrolysis and separation treatment: uniformly mixing the penicillin mushroom dregs with water to perform constant-temperature hydrolysis reaction, and performing solid-liquid separation at a room temperature after the reaction is finished to obtain sediments for later use; and (B), anaerobic digestion treatment: inoculating anaerobic activated sludge in an anaerobic fermentation tank, mixing the sediments obtained in the step (A) with surplus sludge caused by wastewater biological treatment and injecting a mixture into the fermentation tank to stir at a constant temperature, and draining biogas residues and feeding during fermentation. By using the treatment method, residues of drugs in the penicillin mushroom dregs can be completely eliminated, so that the problem that anaerobic fermentation of single penicillin mushroom dreg cannot be efficiently sustained is solved, and minimization and innocent treatment to the penicillin mushroom dregs are realized; and moreover, biogas residues obtained by treatment of the penicillin mushroom dregs can be used as raw materials of organic fertilizers, and biogas generated during anaerobic reaction can be used as cleaning fuels, so that safe and effective treatment and resource utilization of the penicillin mushroom dregs are realized.

The invention provides a preparation method and application of a product obtained by coupling penicillin with carrier protein, as well as a beta-lactam type penicillin antibody. Animals are immunized with penicillin artificial antigen coupled in the invention so as to prepare the antibody which can be used for detecting beta-lactam type penicillin in foods. The preparation method comprises the following steps: immune BALB / C mouse spleen cells and SP2 / 0 mouse myeloma cells are fused; beta-lactam type antibiotics coupled with the carrier protein are used as coating antigen to screen positive hybridoma; hybridoma capable of stably transferring culture and secreting anti-beta-lactam type antibiotic antibodies through cell clones is obtained; and an ascites monoclonal antibody is prepared. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the beta-lactam type antibiotics, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling penicillin with carrier protein, as well as the beta-lactam type antibiotic antibodies can serve the rapid detection of beta-lactam type antibiotic residue in foods.

The invention relates to the antibacterial technology, in particular to a sustained-release penicillin anion intercalation hydrotalcite material as well as preparation and application thereof. The hydrotalcite material has the following chemical composition general formula of [M<2+>1-xM<3+>x(OH)2]<x+>(C16H17N2O4S<->)x.nH2O, wherein M<2+> is the divalent metal ion of Mg<2+>, Zn<2+>, Ni<2+>, Fe<2+> or Mn<2+>, M<3+> is the trivalent metal ion of Al<3+>, Cr<3+>, Fe<3+>, V<3+>, Co<3+>, Ga<3+> or Ti<3+>, the molar ratio of M<2+> / M<3+> is 1.6-4.5, and x is more than or equal to 0.2 and is less than or equal to 0.4. The invention provides the sustained-release penicillin anion intercalation hydrotalcite material and a preparation method thereof. The material is characterized in that penicillin anions are introduced be among hydrotalcite layers; penicillin anion antibacterial agent is released by exchanging anions in the environment and the penicillin anions among the layers to achieve the antibacterial purpose and obviously improve the light and heat stability of penicillin molecules, and the sustained-release penicillin anion intercalation hydrotalcite material has the possibility of serving as the filler of the antibacterial coating to achieve the function of inhibiting microorganism staining.

The invention relates to a pretreatment method for improving anaerobic biogas-production by penicillin slag, and mainly solves the problem that conventional penicillin slag contains a lot of mycelia and a little penicillin residues which inhibit anaerobic digestion to reduce the biogas output. The method comprises the following steps: I, taking dehydrated penicillin slag of a pharmaceutical factory producing antibiotics, then adding distilled water and uniformly stirring to obtain a fermenting raw material, putting the fermenting raw material in a microwave oven, decomposing the fermenting raw material at a high temperature to obtain the decomposed penicillin slag; II, uniformly mixing the decomposed penicillin slag with anaerobic sludge to obtain a mixed liquid and adjusting the pH value and the C / N of the mixed liquid to obtain a fermenting liquid, that is, pretreatment for improving anaerobic biogas-production by penicillin slag is realized. The invention is used for pretreatment method for improving anaerobic biogas-production by penicillin slag.

The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

The invention discloses a process for preparing straight-through 6-aminopenicillanic acid, which comprises the following steps: a, filtering and acidizing penicillin fermentation solution, extracting the penicillin fermentation solution by using butanol, and concentrating and decoloring the extract to obtain butyl ester extracting solution of penicillin; b, back extracting the butyl ester extracting solution of the penicillin by using alkali solution to obtain brine solution of penicillin (heavy phase or RB for short); c, continuously injecting the brine solution of the penicillin into a degreasing tower in a vacuum pressure reduction state to convert the butyl ester into a gas phase from the brine solution of the penicillin, discharging the degreased brine solution of the penicillin out of a pressure reduction system from the bottom of the tower to a storage tank with a cooling device, and cooling the degreased brine solution of the penicillin for later use; and d, performing enzymatic conversion on the degreased brine solution of the penicillin, then adding 6-APA crystal seeds into the solution, growing the crystals, crystallizing the solution, and drying the crystals.

The invention discloses a biological cell freezing antifreeze, and 100ml of the cell freezing antifreeze consists of the following raw materials: 0.025 to 0.3g of kelp polysaccharide, 2.0 to 3.5g of glucose, 0.5 to 1.5g of sodium citrate, 60 to 80ml of distilled water, 10 to 20ml of albumin, 5 to 18ml of glycerol and 0.05 to 0.15 million units of penicillin. The preparation method is that: the glucose and the sodium citrate are dissolved in the distilled water to prepare a base liquid; the albumin is added, the mixture is mixed evenly, and the penicillin is added to prepare a I liquid; the glycerol is added in the I liquid to prepare a II liquid; the kelp polysaccharide is added in the II liquid for even mixing; the pH value is adjusted to 6 to 7.5, then the filtration and sterilization are carried out, and the mixture is cooled until reaching the room temperature and then arranged in a refrigerator of 2 to 5 DEG C for standby. The cell freezing antifreeze is applicable to the cryopreservation of mammalian semen, somatic cells, oocytes, early embryos, organs and tissues, in particular to the cryopreservation of the semen of various mammals.

The invention relates to the field of biomedicine and in particular relates to an antibacterial polypeptide. Part of active amino acids of the antibacterial polypeptide of Cathelicidin family is mutated based on the antibacterial polypeptide of Cathelicidin family, and then the antibacterial polypeptide with high inhibitory activity to penicilin-resistant bacterium is obtained by solid phase chemical synthesis. The result of pharmacodynamic tests shows that the antibacterial polypeptide provided by the invention has good in vitro antibacterial activity and is applicable to treating diseases about drug-resistant bacterial infection.

The invention provides a mutant of penicillin G acylase (PGA) and a preparation method and application of the mutant. The non-rationally and semi-rationally designed enzyme engineering reconstruction technology is adopted for mutation of penicillin G acylase obtained from Escherichia coli ATCC 11105, so that a PGA mutant with higher reactivity, higher reaction rate, better conversion rate, stronger in penicillihe concentration tolerance, less substrate residue, and higher in phenyl acetic acid concentration tolerance; meanwhile, the mutant is subjected to recombinant expression, bacteria strain construction, fermenting cultivation, immobilization and application to prepare 6-amino-penicillanic acid (6-APA). The activity of the PGA-6 mutant prepared by the invention is increased by 102 times, the substrate penicillihe concentration tolerance is increased to 30%, and the phenyl acetic acid concentration tolerance is increased to 20 mmol / L; meanwhile, the immobilized PGA-6 mutant is used to decompose penicillihe with a concentration of 25% under the condition of pH 8.0 and 25 DEG C so as to prepare 6-APA, and the reaction time is shortened to 55 minutes, the substrate conversion rate is 98% or above, and after being used for 600 batches and above, the activity is not lost obviously, therefore, good operation stability is achieved.

An organic and inorganic compound fertilizer special for tobacco for inducing tobacco to resist diseases and the application thereof pertain to biological technical field. The weight proportion of each component of the compound fertilizer is that 21-28 percent organic matters, 7.7-9.4 percent full nitrogen, 4.3-7 percent P, 12-15 percent K and 47.6-48.2 percent for the rest. The preparation method is that: lignite is first heated under 200-300 DEG C to be dried; solid and liquid waste residues for producing penicillin in medicine industry and silkworm feces are mixed with dried lignite according to the weight proportion of lignite, silkworm feces and waste residues of 5-10 to 1-5 to 1; the mixture is then kept in a temperature of above 85 DEG C for at least 20 minutes; then ammonium nitrate phosphate fertilizer, potassium sulfate, monoammonium phosphate, calcium-magnesium phosphate, ferrous sulfate and the organic mixture are mixed according to the proportion of 24-28 : 24-30 : 5-10 : 4-6 : 0-0.5 : 25.7-43 to obtain compound fertilizer. The compound fertilizer is applied for inducing tobacco to express albumen related to the course of disease and thereby producing disease resistance of the system and providing nutrition requirements for growth of tobacco. The invention has the advantages of good effects and easy application and is applicable for the production of pollution-free tobacco.

The invention belongs to the field of biodegradation treatment, and provides a penicillin sodium degrading bacterium, separation and identification of the degrading bacterium and application to penicillin sodium degradation of the degrading bacterium. The penicillin sodium degrading bacterium belongs to chelatococcus, the classified name is Chelatococcus sp., the preservation serial number is CGMCC No.1.15283, and the penicillin sodium degrading bacterium is preserved in China microbial strain preservation management council common microorganism center on June 11th, 2015. Degradation characteristic research shows that through 12-d shaking culture at the temperature of 37 DEG C on the condition of 150 r / min, the degradation rate of PC-2 to penicillin sodium with the initial concentration of 200 ppm is 53.5%. When glucose serves as a carbon source, peptone serves as a nitrogen source, the degrading bacterium inoculum size is 14%, the initial concentration of penicillin sodium is 100 ppm, and the pH value range is 6.0-7.0, the degradation rate is the largest. The penicillin sodium degrading bacterium has a good degradation characteristic to penicillin sodium, and provides a certain reference basis for degrading penicillin residual through a biological method.

The invention belongs to the technical field of animal tissue isolation and culturing, and particularly relates to a method of porcine intestinal crypt isolation and 3D type organ culturing. In the course of porcine intestinal crypt isolation, penicillin and streptomycin are added, so that in the later-stage culturing course, pollution caused by intestinal microorganisms is avoided; rotating speedand time for treating an intestinal segment of a horizontal rotary instrument and frequency and time of a vortex of a vortex instrument are optimized, so that the obtained crypt is complete in shape,and higher in survival rate and bud rate. According to the method disclosed by the invention, a formula of a culture solution for culturing the crypt and the 3D type organs is optimized, so that thecrypt can be well polarized to form the 3D type organs, and the growth and the polarization are stable; the isolating condition and the optimal culturing method of the porcine intestinal crypt are determined, and favorable foundation for subsequently utilizing the porcine intestinal 3D type organs to study enterovirus and bacterial infection is established.

The invention discloses a preparation method of an egg yolk antibody against citrinin, comprising the following steps: (1) to prepare the artificial antibody of citrinin; (2) to inject the artificial antibody of citrinin into a hen body to immune; and after 4 times of immunity, to collect eggs continuously for 1 to 2 months; and (3) to extract the egg yolk antibody against citrinin. The prepared egg yolk antibody of anti-citrinin has the advantages of low preparation cost, large productivity, and high specificity. The method can be applied to the development of gold colloid and the establishment of fast examination technology with rapidness, sensitivity, specificity to the citrinin, and has a great application prospect in the safety examination of agricultural products and foods.

The invention provides a hapten comprising a 6-[D-alpha-aminoacetamido]penicillin derivative crosslinked at the alpha-amino group with a substituted or unsubstituted phenyldicarbaldehyde. In addition, the invention provides an immunogen comprising the aforementioned hapten coupled to an antigenicity-conferring carrier material, a conjugate comprising the aforementioned hapten coupled to a labelling agent, as well as, antibodies raised against the aforementioned immunogen and capable of binding with at least one structural epitope of an intact beta-lactam ring. The invention further provides a method and a kit for detecting, or determining the quantity of, beta-lactam antibiotics, as well as, use of the aforementioned conjugate with the aforementioned antibodies for detecting, or determining the quantity of, beta-lactam antibiotics. The present invention has broad specificity across the main first generation beta-lactams and can be used to test milk and meat and the like for the presence of residual beta-lactam antibiotics.

The invention discloses a culture method of rat myocardial cell. The culture method of the rat myocardial cell comprises the following steps: (1) soaking rat myocardial tissue in digestive enzyme mixed liquid, carrying out fractional digestion, removing digestion liquid until the myocardial tissue is loose and soft, then adding the loose and soft myocardial tissue into a DMEM (Dulbecco Modified Eagle Medium) culture medium, blowing and beating the loose and soft myocardial tissue and taking supernatant for centrifuging so as to obtain a cell sediment; (2) re-suspending the cell sediment by using the DMEM culture medium, passing a cell suspension through a sieve with 180-220 meshes, subsequently transferring the cell suspension to a culture flask and carrying out differential attachment 2-3 times for 40-50 minutes per time; (3) inoculating the myocardial cell in a culture vessel with a density of (1.5-2.5)*10 <5>cells / mL, and putting the culture vessel in a culture box for culturing after inoculating. The method is low in cost, short in time and simple in process; drugs such as penicillin, Brdu and the like are not used, so that the potential impact of penicillin and Brdu on the subsequent research can be avoided.

The invention relates to a novel cellulose-based antibacterial material, which is mainly produced via the steps of: controlling reaction temperature, time, pH value and oxidization ratio of dialdehydecellulose in order to perform a reaction to the dialdehyde cellulose and lysine, and when the reaction is finished, performing centrifugal separation and drying and grinding the product. The antibacterial material has excellent antibacterial activity, and can reach 30 mg / ml in minimum inhibitory concentration (MIC) against Gram negative bacteria, such as Escherichia coli, and 7.5 mg / ml in MIC against Gram positive bacteria, such as staphylococcus aureus, with penicillin as a positive control and an LB liquid culture medium as a negative control; under special conditions, the antibacterial material can completely growth of bacteria. The antibacterial material has simple process and is free of toxic and side effect. The raw material, cellulose, has abundant sources and is environment friendly. The invention supplies a new idea for researching and developing novel antibacterial materials.

The invention relates to application of natural compound ursolic acid on antibiosis, in particular to application of the combination of the natural compound ursolic acid and penicillin on resisting drug resistant staphylococcus aureus. The compound ursolic acid is widely distributed in nature, so that the material source is wide. Aiming at the serious problem of the drug resistance of the staphylococcus aureus at present, the in vitro and in vivo antibiotic tests are carried out for the ursolic acid. Particularly the in vitro synergic test proves that the ursolic acid has better antibacterial activity on the drug resistant staphylococcus aureus under the combination action of the ursolic acid and the penicillin, and the lowest bacteriostatic concentration is 1.13 mu g / disc; moreover, the in vivo test also shows that the ursolic acid has better activity under the synergic action.

The invention relates to a method for preparing penicillin-G-1-(S)-oxide. In the method, penicillin G is used as a raw material, and the oxidization of the penicillin G and the crystallization of penicillin-G-1-(S)-oxide which is the oxidization product of the penicillin G are performed at the same time. A penicillin-G-1-(S)-oxide crystal product is obtained by directly adding an oxidant into penicillin G organic phase solution, reacting and crystallizing, the oxidization reaction and the crystallization are performed at the same time, and the crystal product directly precipitates in organic solution. Compared with the conventional direct oxidization process, the method has the advantages that: the operation flow is simplified considerably; the operation is simple and convenient; the labor intensity is lowered; and the production period is shortened obviously. The process of extracting the penicillin G from the organic phase back to a water phase and the process of regulating pH value with diluted acid and crystallizing are saved, so the consumption of waster resources is reduced greatly, the discharge of waste acid liquor is reduced, and the problem of environmental pollution is alleviated obviously. The high-performance liquid chromatography (HPLC) content of the product reaches over 99.0 percent, and the weight yield of products with a main particle size over 30 mu m is over 90 percent.