The invention provides a preparing method of D-p-hydroxyphenylglycine, which comprises adding DL-p-hydroxyphenylglycine and resolving agent beta-naphthalenesulfonic acid in water to prepare solution, rising temperature and reacting under agitating, adding induction seeds, adjusting the specific totation of the solution, performing heat-preservation reaction, cooling, sufficiently crystallizing, reducing pressure, filtering and drying, thereby obatining solid D-p-hydroxyphenylglycine beta-naphthalenesulfonic acid complex salts or L-p-hydroxyphenylglycine beta naphthalenesulfonic acid complex salts; dissolving D-p-hydroxyphenylglycine beta-naphthalenesulfonic acid complex salts in water to prepare aqueous solution, rising temperature, adding active carbon to perform decoloring treatment, adjusting the PH value by alkali liquid, cooling the sollution to room temperature, filtering, washing, and drying, thereby obatining D-p-hydroxyphenylglycine. The D-p-hydroxyphenylglycine produced by the method of the invention has good quality, short reaction time and low cost.