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Application of natural compound ursolic acid on antibiosis

A natural compound, ursolic acid technology, applied in the direction of active ingredients, applications, and antibacterial drugs of heterocyclic compounds, to achieve good antibacterial activity, wide distribution, and rich sources of raw materials

Inactive Publication Date: 2009-09-30
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on the activity of ursolic acid are mainly anti-tumor, and there are also reports on antibacterial aspects, such as Indian scholar Chattopadhyay D (Antimicrobial and anti-inflammatory activity offolklore: Mallotus peltatus leaf extract. J Ethnopharmacol, 2002, 82.) The in vitro antibacterial test of the methanol extract of the leaves of the genus Tunga genus, found that ursolic acid, as one of the main components, has inhibitory effects on various bacteria such as Staphylococcus aureus, Staphylococcus saprophyticus and Escherichia coli, but these antibacterial reports are only Involved in the general screening of antibacterial activity, did not involve whether it can be antibacterial in vivo and in vitro

Method used

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  • Application of natural compound ursolic acid on antibiosis
  • Application of natural compound ursolic acid on antibiosis
  • Application of natural compound ursolic acid on antibiosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Anti-Staphylococcus aureus activity study:

[0016] The minimum inhibitory concentration MIC and the minimum bactericidal concentration MBC research of compound ursolic acid in Lilac yunnanensis to Staphylococcus aureus, medium is agar, contrast is penicillin, MIC and MBC are shown in table 1 respectively, table 2 (MIC is expressed on the medium The highest dilution of the reagent that grows completely aseptically is taken as the minimum inhibitory concentration of the drug; for MBC, the highest concentration of the reagent that grows completely aseptically on the medium is used as the minimum bactericidal concentration of the reagent).

[0017] Table 1 MIC test of antibacterial activity of compound ursolic acid in Lilac yunnanensis against Staphylococcus aureus strains

[0018]

[0019] Note: +: bacterial growth; -: sterile growth

[0020] Table 2 MBC test of the antibacterial activity of ursolic acid compound in Lilac yunnanensis against Staphylococcus aureus stra...

Embodiment 2

[0025] Activity against resistant Staphylococcus aureus:

[0026] The disc diffusion method was used to detect the activity of ursolic acid isolated from the plant Lilac yunnanensis against drug-resistant strains, and it was found that it had no inhibitory activity against drug-resistant Staphylococcus aureus. In view of this, the present invention carries out synergy experiment with ursolic acid and penicillin.

Embodiment 3

[0028] Synergy test:

[0029] The combined use of antibiotics and the widespread clinical use of synergists have made the synergistic theory more and more perfect. According to the antibacterial activity of the combined antibiotics, there is a significant difference depending on the components of each antibiotic in the combined drug, and the antibiotic effect after the combined use is judged: when the antibacterial activity after the combined drug is higher than the sum of the antibacterial activities of its components , a synergistic effect is produced.

[0030] Mix ursolic acid and penicillin Serva31749 in the ratio of 1:1; 2:1; 3:1 by weight to find the optimal ratio. The results are shown in Table 3.

[0031] Table 3 Screening of the optimal ratio of compound and penicillin synergistic effect

[0032]

[0033] Note: the average value of three tests; D: the diameter of the inhibition zone; Con: the original concentration, that is, the concentration before dilution, in...

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Abstract

The invention relates to application of natural compound ursolic acid on antibiosis, in particular to application of the combination of the natural compound ursolic acid and penicillin on resisting drug resistant staphylococcus aureus. The compound ursolic acid is widely distributed in nature, so that the material source is wide. Aiming at the serious problem of the drug resistance of the staphylococcus aureus at present, the in vitro and in vivo antibiotic tests are carried out for the ursolic acid. Particularly the in vitro synergic test proves that the ursolic acid has better antibacterial activity on the drug resistant staphylococcus aureus under the combination action of the ursolic acid and the penicillin, and the lowest bacteriostatic concentration is 1.13 mu g / disc; moreover, the in vivo test also shows that the ursolic acid has better activity under the synergic action.

Description

technical field [0001] The invention relates to an application of a natural compound ursolic acid in antibacterial aspects, in particular to an application of the compound in synergistic anti-drug-resistant bacteria in vivo and in vitro. Background technique [0002] Staphylococcus aureus is one of the main pathogens of nosocomial infection. Staphylococcus aureus can cause skin infection from small to severe pneumonia, meningitis, bone and joint infection, endocarditis and bacteremia that can be fatal. Patients with end-stage renal disease, who are often treated with hemodialysis, are particularly susceptible to S. aureus infection, a leading cause of infection and death in hospitalized patients. With the continuous advent of new antibiotics, the drug resistance of Staphylococcus aureus has gradually increased, which has become a thorny problem in clinical treatment. At present, the research and development of new antibacterial drugs are mainly synthesis, semi-synthesis and ...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61K31/43A61P31/04A01N45/00A01P1/00
Inventor 康文艺宋艳丽姬志强王金梅
Owner HENAN UNIVERSITY
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