The invention relates to a method for quickly detecting
drug resistance of
bacteria. The method comprises the following steps: experimental groups of
antibiotics with different concentrations, a
positive control group and a
negative control group are set, wherein the experimental groups of the
antibiotics with the different concentrations refer to experimental groups of adding the
antibiotics withthe different concentrations into
culture mediums and adding
heavy water in subsequent steps, the
positive control group refers to the experimental group of adding antibiotics with a concentration of0 into a culture medium and adding
heavy water in subsequent steps, and the
negative control group refers to the experimental group of adding antibiotics with a concentration of 0 intp a culture medium without adding
heavy water in subsequent steps, after incubation is performed, centrifugal washing is performed, samples are subjected to Raman detection, values of C-D / (C-D + C-H) are calculated respectively according to the obtained Raman spectra, a turning point of value decrease of C-D / (C-D + C-H) of the experimental groups of the antibiotics with different concentrations relative to the control groups is taken as a
minimum inhibitory concentration of the antibiotics on the
bacteria, and the
minimum inhibitory concentration is compared with a
breakpoint given in a CLSI susceptibility test standard to determine the bacterial sensitivity, intermediation or
drug resistance. The method introduces a
breakpoint comparison to more accurately give the sensitivity of the
bacteria to the antibiotics.