41 results about "Acidithiobacillus thiooxidans" patented technology

The invention publishes a process to increase the bioleaching speed of ores or concentrates of sulfide metal species in heaps, tailing dams, dumps, or other on-site operations. The process is characterized by the continuous inoculation of the ores or concentrates with isolated microorganisms of the Acidithiobacillus thiooxidans type, together with isolated microorganisms of the Acidithiobacillus ferrooxidans type, with or without native microorganisms, in such a way that the total concentration of microorganisms in the continuous inoculation flow is of around 1×107 cells / ml to 5,6×107 cells / ml. In particular, the invention publishes the continuous inoculation of Acidithiobacillus thiooxidans Licanantay DSM 17318 together with Acidithiobacillus ferrooxidans Wenelen DSM 16786 microorganisms, or with other native microorganisms at a concentration higher than 5×107 cells / ml. In addition to the inoculation of isolated bacteria, the invention includes the addition of oxidizing agents such as the ferric ion produced externally, together with nutrients in the shape of salts of ammonium, magnesium, iron, potassium, as well as air enriched continuously with carbon dioxide to promote bacterial action in the bioleaching process of ores or concentrates.

The invention publishes a reactor and method for the culture, biooxidation of cations in solution and / or large-scale propagation of jointly isolated microorganisms, with or without native microorganisms that are useful in sulfide metal ore bioleaching. The invention particularly publishes a reactor for the large-scale culture and / or propagation of an association of Acidithiobacillus thiooxidans Licanantay DSM 17318 isolated microorganisms jointly with Acidithiobacillus ferrooxidans Wenelen DSM 16786 with or without the presence of other microorganisms.

The invention relates to Acidithiobacillus thiooxidans and a heap leaching technology using same of the middle-grade and low-grade phosphate ore. The name of the bacteria is Acidithiobacillus thiooxidans RETECH-PS1; the bacteria are conserved in the China center for type culture collection of which address is the Wuhan University; the conservation date is April 25, 2009; and the CCTCC NO is 209088. The biological heap leaching technology of the middle-grade and low-grade phosphate ore comprises the following steps: ores are crushed to less than 15mm, sulfur is added to mix and build up leaching heap; (2) the Acidithiobacillus thiooxidans in the claim 1 which is conserved at low temperature is activated and propagated step by step to reach the bacterium solution volume for spraying, finally the bacteria are inoculated in the middle-grade and low-grade phosphate ore heap mixed with sulfur; and (3) leachate is collected by a collection pool and pumped by a pump to a spraying system for circular spraying. The invention has the following advantages: the process flow is simple, the middle-grade and low-grade phosphate ore is used without adopting ore dressing and enrichment; sulfuric acid is not added in the production process of phosphoric acid, thus reducing the cost and avoiding the environmental pollution problem caused by the production of sulfuric acid; the production scale can be large or small; and the technology is especially suitable for the collophanite resource which accounts for 70% of Chinese phosphorus resources and is difficult to treat through traditional ore dressing, thus increasing the comprehensive utilization rate of Chinese phosphorus resources.

The invention belongs to the technical field of microbe, and particularly relates to a method for treating radionuclide plutonium or strontium polluted soil by microbe. The method comprises the following steps: screening the plutonium or strontium polluted soil by a bar screen, a double-plate screen and a spiral screen, wherein the soil grains with the granularity of more than 0.25 millimeter areclean soil grains and the soil grains with the granularity of less than 0.25 millimeter are plutonium or strontium polluted soil grains; and mixing the soil grains with the granularity of less than 0.25 millimeter and acidithiobacillus thiooxidans active solution, filtering the solution to obtain sediment which is clean soil, mixing the filtered solution and sulfate reducing bacteria active solution, settling plutonium or strontium, filtering and separating the solution, reclaiming the plutonium or the strontium, and recycling the water. The method of the invention has the advantages of simple and convenient operation, economy and feasibility and high safety, has no secondary pollution, and can efficiently and quickly remove the plutonium or the strontium from the polluted soil.

The invention discloses the use of mining products and sub-products that contain pyrite, such as copper concentrates, and waste from the process in which these concentrates are obtained, known as scavenger tail, as an energy source for the large-scale culture of an association of microorganisms that are useful for ore bioleaching, and that includes both isolated microorganisms, and native microorganisms from the worked ores. In particular, the invention publishes the use of mining waste known as scavenger tail from the flotation process, in the culture of an association of isolated microorganisms of the Acidithiobacillus ferrooxidans y Acidithiobacillus thiooxidans type together, with or without other native microorganisms from the worked ores.

The invention discloses a method for screening an unmarked gene knockout bacterial strain of acidithiobacillus thiooxidans. The method comprises introducing a knockout plasmid for construction of a target gene and an induction plasmid carrying an I-SceI gene into acidithiobacillus thiooxidans by conjugational transfer, carrying out single recon screening by a resistance maker, and carrying out double recon preliminary screening by blue-white selection. The method discloses the method for unmarked gene knockout of acidithiobacillus thiooxidans first, utilizes beta-galactosidase blue-white selection for double recon screening, can fast, stably and efficiently knock out a target gene, solves the problem that in the existing unmarked gene knockout process, selective pressure lacks so that screening is difficult, shortens double recon screening time, improves screening efficiency, reduces a screening cost and provides a novel method for stable genetic modification of acidithiobacillus thiooxidans.

A microorganism which can remove the malodorous gas of NH3 and H2S synchronously and a preparation method thereof relate to the microorganism technical field and in particular to an efficient deodorization microorganism of the mixed malodorous gas in a sewage collecting and disposing establishment and the preparation method thereof. An acidithiobacillus thiooxidans CCW-Y2 strain provided by the present invention is conserved in the General Microorganism Center of China Committee for Culture Collections of Microorganisms, and the conversing serial number CGMCC No. is 2158. The strain is ammonium sulfate tolerant type and is obtained by sieving and separating from the activated sludge of the living sewage disposal factory. The culture conditions of the acidithiobacillus thiooxidans CCW-Y2 strain of the present invention are as follows: firstly, SOB culture medium is adopted, and the components of the culture medium are the dipotassium hydrogen phosphate of 2g / L, the potassium dihydrogen phosphate of 2g / L, the magnesium chloride (MgCl2 / 7H2O) of 0.2g / L, the ammonium sulfate of 10g / L, the ferrous sulfate (FeSO4 / 7H2O) of 0.01g / L and the powdered sulfur of 10g / L; secondly, the culture condition is the temperature of 28 DEG C to 32 DEG C and 180 circles per minute; thirdly, a carrier is water, and dilute sulfuric acid (or hydrochloric acid) is added for adjusting pH value to be 2.0 to 3.0. The acidithiobacillus thiooxidans CCW-Y2 strain of the present invention can be directly inoculated into a waste gas disposing establishment (such as a biological filtration tower) and can synchronously remove the mixed malodorous gas of the H2S and the NH3 efficiently, which simplifies the operation condition of the waste gas disposing establishment and ensures that the operation is more simple.