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Acidithiobacillus thiooxidans and heap leaching technology using same of middle-grade and low-grade phosphate ore

A technology of thiothiobacillus and acid oxidation, which is applied in the field of microorganisms, can solve the problems of lengthy phosphate fertilizer production process, difficulty in direct processing of phosphate rock, high cost of beneficiation, etc., achieve good leaching effect, reduce beneficiation cost, and low cost

Active Publication Date: 2012-11-14
有研资源环境技术研究院(北京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production process of phosphate fertilizer is lengthy and cumbersome, and the production process of sulfuric acid is also required, causing environmental pollution
More importantly, with the consumption of rich phosphate rock, the existing medium and low-grade phosphate rock is difficult to process directly, and the cost of beneficiation is increasing

Method used

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  • Acidithiobacillus thiooxidans and heap leaching technology using same of middle-grade and low-grade phosphate ore

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] First, the low-temperature-preserved phosphoacidophilic thiothiooxidans strain (the bacterial name is Acidithiobacillusthiooxidans RETECH-PS1, preservation unit: China Collection Center for Typical Cultures, address: inside Wuhan University, preservation date: April 25, 2009 , deposit registration number: CCTCC NO: M 209088) in 10ml medium (0.1g KCl, 3g NH 4 SO 4 , 0.5g K 2 HPO 4 ·3H 2 O, 0.5g Mg(SO 4 ) 2 ·7H 2 O and 0.01g Ca(NO 3 ) 2 ) was dissolved in 1L distilled water, and activated in a test tube supplemented with elemental sulfur according to the addition amount of 10g / L. The activated strains were transferred into 100ml culture solution, and after 24 hours of cultivation, the inoculation amount of 10% (v / v) was gradually expanded to the amount required for heap leaching. During the cultivation process, the pH was controlled to be 2.0 and the temperature was 28°C. .

[0025] Phosphate rock to be treated (P 2 o 5 =18.98% by weight) broken down to less t...

Embodiment 2

[0027] First, the low-temperature-preserved phosphoacidophilic thiothiooxidans strain (the bacterial name is Acidithiobacillusthiooxidans RETECH-PS1, preservation unit: China Collection Center for Typical Cultures, address: inside Wuhan University, preservation date: April 25, 2009 , deposit registration number: CCTCC NO: M 209088) in 10ml medium (0.1g KCl, 3g NH 4 SO 4 , 0.5g K 2 HPO 4 ·3H 2 O, 0.5g Mg(SO 4 ) 2 ·7H 2 O and 0.01g Ca(NO 3 ) 2 dissolved in 1L distilled water, and supplemented with elemental sulfur according to the addition amount of 10g / L) in a test tube for activation. The activated strains were transferred into 100ml of culture solution, and after 24 hours of cultivation, the inoculation amount of 10% (v / v) was gradually expanded to the amount required for heap leaching. During the cultivation process, the pH was controlled to be 2.5 and the temperature was 30°C. .

[0028] Phosphate rock to be treated (P 2 o 5 =21.32% by weight) broken down to les...

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Abstract

The invention relates to Acidithiobacillus thiooxidans and a heap leaching technology using same of the middle-grade and low-grade phosphate ore. The name of the bacteria is Acidithiobacillus thiooxidans RETECH-PS1; the bacteria are conserved in the China center for type culture collection of which address is the Wuhan University; the conservation date is April 25, 2009; and the CCTCC NO is 209088. The biological heap leaching technology of the middle-grade and low-grade phosphate ore comprises the following steps: ores are crushed to less than 15mm, sulfur is added to mix and build up leaching heap; (2) the Acidithiobacillus thiooxidans in the claim 1 which is conserved at low temperature is activated and propagated step by step to reach the bacterium solution volume for spraying, finally the bacteria are inoculated in the middle-grade and low-grade phosphate ore heap mixed with sulfur; and (3) leachate is collected by a collection pool and pumped by a pump to a spraying system for circular spraying. The invention has the following advantages: the process flow is simple, the middle-grade and low-grade phosphate ore is used without adopting ore dressing and enrichment; sulfuric acid is not added in the production process of phosphoric acid, thus reducing the cost and avoiding the environmental pollution problem caused by the production of sulfuric acid; the production scale can be large or small; and the technology is especially suitable for the collophanite resource which accounts for 70% of Chinese phosphorus resources and is difficult to treat through traditional ore dressing, thus increasing the comprehensive utilization rate of Chinese phosphorus resources.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. In particular, a strain of phosphoacidophilic thiothiooxidans with elemental sulfur biooxidation function and its heap leaching process for medium and low-grade phosphate rock. Background technique [0002] Phosphate fertilizer is one of the three basic raw material fertilizers of nitrogen, phosphorus and potassium for agricultural production in my country, and it is also an important guarantee for the safety of national grain reserves. Traditional phosphate fertilizer production uses beneficiation technology to improve the grade of phosphate rock, then adds concentrated sulfuric acid to decompose phosphate rock to produce phosphoric acid, and then uses phosphoric acid to make phosphate fertilizer. The production process of phosphate fertilizer is lengthy and cumbersome, and the production process of sulfuric acid is also required, causing environmental pollution. More critically, with t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C22B3/18C12R1/01
CPCY02P10/20
Inventor 温建康杨均流刘兴宇林大泽张永德陈勃伟
Owner 有研资源环境技术研究院(北京)有限公司
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