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Sulfur oxide thiobacillus and application thereof in heavy metal removal

A technology of Thiobacillus thiooxidans and heavy metals, which is applied in the restoration of bacteria and polluted soil, and methods based on microorganisms, can solve the problems of inability to be directly utilized, high cost, low efficiency, etc. The effect of low cost and high processing efficiency

Inactive Publication Date: 2017-06-30
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the invention: to provide a microorganism that can directly remove heavy metals and its application method mainly for the waste containing a large amount of heavy metals that cannot be directly used, and the current removal methods have the problems of low efficiency and high cost

Method used

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  • Sulfur oxide thiobacillus and application thereof in heavy metal removal
  • Sulfur oxide thiobacillus and application thereof in heavy metal removal
  • Sulfur oxide thiobacillus and application thereof in heavy metal removal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Screening method for Acidithiobacillus thiooxidans ZJ-1;

[0037] Weigh 10g of pickling sludge sample, put it into a conical flask filled with 100ml (121°C, 30min wet sterilized) pure liquid medium (SM medium), add sulfur powder to it, and wait for the bottle to When the pH value of the medium turbidity drops to about 2.0, after standing still, absorb 10mL of the supernatant, inoculate it into a 250mL Erlenmeyer flask filled with 100mL of wet-sterilized SM medium, add 10g of sulfur powder, and shake at a constant temperature. Cultivate in bed (30°C, 150rpm); cultivate for 8d-10d, until the culture medium becomes turbid milky white (pH value is about 2). Draw 10mL from it and inoculate it into fresh SM medium, and repeat the enrichment culture for 3-4 times, so that acidophilic Thiobacillus thiooxidans becomes the dominant bacterial group; dilute the enriched medium and apply it to solid SM culture After the coated petri dish was placed at room temperature for 2 hours, ...

Embodiment 2

[0039] Removal of heavy metals from contaminated soils;

[0040] Take the sample to be tested and add water to make a mixed slurry, and determine the concentration of various heavy metals in it. Add 120mL slurry to each 250mL Erlenmeyer flask, and add a small amount of sulfur and ammonium sulfate to it, add the bacterium solution that has been acclimatized to the solid waste slurry in an amount of 20%, to add the solid waste of the inactivated bacterium solution. The waste slurry was used as a blank control group, and the concentration of heavy metal ions in the experimental group and the blank group were monitored respectively, and the ratio of the precipitated concentration to the heavy metal concentration in the slurry was used as the removal rate. Such as Figure 4 -7.

[0041] Table 1 List of 16s RNA sequences of acidophilic Thiobacillus thiooxidans ZJ-1:

[0042]AGGAATCTGTCTTTGAGTGGGGGACAACCCAGGGAAACTTGGGCTAATACCGCATAAGCCCTGAGGGGGAAAGCGGGGGATCTTCGGACCTCGCGCTGGAAGAGGAG...

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Abstract

The invention relates to a sulfur oxide thiobacillus Acidithiobacillus thiooxidans ZJ-1 and an application thereof in heavy metal removal. The bacterial strain is preserved in the China Microbial Culture Preservation Management Committee General Microbiology Center, the preservation number is CGMCC No. 12387. The optimum growth temperature of the bacterial strain ZJ-1 is 28 DEG C, and the optimal growth pH value is 3.0. The bacterial strains can separate out the heavy metals in all kinds of wastes, can be used for the removal of heavy metals in all kinds of wastes and has an important function for preventing the secondary pollution of heavy metals.

Description

technical field [0001] The invention belongs to the field of biological treatment, and in particular relates to a microorganism capable of removing heavy metals and its application field. Background technique [0002] Various wastes such as municipal sludge often contain a large amount of organic matter and nutrients needed by plants, and have extremely high agricultural value. However, sludge usually contains a large amount of heavy metals, which are very difficult to be biodegraded and are easily bioaccumulated through the food chain. Eating vegetables irrigated by such sludge will seriously threaten people's health. [0003] Therefore, we usually need to deal with heavy metals so that they can be used more safely and effectively. In the actual treatment process, the use of physical and chemical methods generally has the problems of long time-consuming, high cost, and easy to produce secondary pollution. Therefore, more and more people tend to use biological methods to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCC12N1/20B09C1/10C12R2001/01C12N1/205
Inventor 周俊杨俊张雪英
Owner NANJING UNIV OF TECH
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